TRANSGENIC SOYBEAN EVENT MON 87708 AND METHODS OF USE THEREOF

Abstract

Claim 1: A recombinant DNA molecule comprising a nucleotide molecule comprising a nucleotide sequence selected from the group consisting of SEC ID Nos. 1-8, and its complements. Claim 8: A method for detecting the presence of a DNA molecule derived from the MON 87708 soybean event in a sample, said method comprising: a) contacting a sample with the DNA probe of claim 6; b) subjecting said sample and said DNA probe to severe hybridization conditions; and c) detecting the hybridization of said DNA probe to a DNA molecule in said sample, wherein hybridization of said DNA probe to said DNA molecule indicates the presence of a DNA molecule derived from the MON 87708 soybean event in said sample.Claim 11: A recombinant soybean plant, seed, cell, or part thereof comprising a nucleotide molecule having a nucleotide sequence selected from the group consisting of SEC ID Nos. 1-8, and its complements. Claim 20: The commodity according to claim 19, wherein said commodity is selected from the group consisting of whole or processed seeds, animal feed, oil, ground grain, flour, flakes, bran, milk, cheese, paper, cream, wine, biomass, and fuel products. Claim 21: A method for weed control in a field comprising planting plants of the MON 87708 soybean event in a field and applying an effective dose of dicamba herbicide to control weeds in said field without harming said plants of the MON 87708 soybean event.

Description

-03'00' TRANSGENIC SOYBEAN EVENT MON 87708 AND METHODS OF USE THEREOF CROSS REFERENCE TO RELATED APPLICATIONS This application claims the benefit of U.S. Provisional Application No. 61 / 243.227 filed on September 17, 2009, which is incorporated herein by reference in its entirety. INCORPORATION OF SEQUENCE LIST The sequence list contained in the file named “55544-0001_seqlisting.txt”, which is 19.5 kilobytes in size (as measured in Microsoft Windows®) and was created on August 13, 2010, is submitted with this application by electronic submission and is incorporated as a reference in this invention. FIELD OF INVENTION The invention relates to the transgenic event of Glycine max MON 87708. This event exhibits tolerance to the herbicide dicamba. The invention also relates to plants, plant parts, plant seeds, plant cells, agricultural products, and methods related to the MON 87708 event and provides nucleotide molecules that are unique to the event and were created in connection with the insertion of transgenic DNA into the genome of a Glycine max plant. BACKGROUND OF THE INVENTION Soybeans (Glycine max) are an important crop in many parts of the world, and biotechnology methods have been applied to this crop to produce soybeans with desirable traits. One such desirable trait is herbicide tolerance. Expressing a herbicide tolerance transgene in a plant can confer this desirable trait, but the expression of the transgene can be influenced. IF-2019-80266805-APN-ANP#INPI Page 1 of 50 due to the chromosomal location and the genomic outcome of the transgene insertion. For example, it has been observed in plants that there is often variation in the level and pattern of transgenic expression among individual events that differ in the chromosomal insertion site of the transgene but are otherwise identical. There may also be undesirable and / or desirable phenotypic or agronomic differences between events. Because of this, it is often necessary to produce and analyze a large number of transformation events from individual plants in order to select an event that has both the desired trait and the optimal phenotypic and agricultural characteristics necessary to make it suitable for commercial purposes.Such selection often requires greenhouse and field trials with numerous events over several years, at multiple locations, and under a variety of conditions so that a significant amount of agronomic, phenotypic, and molecular data can be collected. The resulting data and observations must then be analyzed by teams of scientists and agronomists to select a commercially viable event. Once selected, this type of event can then be used to introgress the desirable trait into other genetic backgrounds using plant breeding methods, thus producing a number of different crop varieties that possess the desirable trait and are well-suited to specific local growing conditions. SUMMARY OF THE INVENTION The invention provides transgenic soybean plants designated event MON 87708, which exhibit commercially acceptable tolerance to applications of dicamba herbicide, the representative seed having been deposited with the American Type Culture Collection (ATCC) with Accession No. IF-2019-80266805-APN-ANP#INPI Page 2 of 50 PTA-9670. The invention further provides novel DNA molecules related to the MON 87708 soybean event and methods for using these molecules. The invention also provides seeds, progeny, plant parts, cells, and raw materials from the MON 87708 soybean event. The invention further provides methods for using the MON 87708 soybean event and methods for producing dicamba-tolerant soybeans. The invention provides recombinant DNA molecules related to the MON 87708 soybean event. These recombinant DNA molecules may comprise nucleotide molecules having a nucleotide sequence representing a region of genomic DNA flanking the transgene insertion, and / or a region of the transgene insertion, and / or a contiguous sequence of any of these regions, such as a junction region between the transgenic insertion and the flanking genomic DNA of the MON 87708 soybean event. The invention further provides DNA molecules useful as primers and probes for diagnosing the MON 87708 soybean event and diagnostic amplicons for the presence of the MON 87708 soybean event. Soybean plants, plant cells, plant parts, consumer goods, progeny, and seeds comprising these molecules are further described. The invention provides methods, compositions, and kits useful for detecting the presence and / or absence of DNA derived from the MON 87708 soybean event and, therefore, the presence and / or absence of the event. The invention provides a method for detecting MON 87708 by contacting a sample comprising DNA with a primer set that, when employed in a nucleic acid amplification reaction with genomic DNA from the MON 87708 soybean event, produces diagnostic amplified DNA of the MON 87708 soybean event, carrying out a nucleic acid amplification reaction thereby producing the amplified DNA, and detecting the presence and / or IF-2019-80266805-APN-ANP#INPI Page 3 of 50 absence of amplified DNA. The invention further provides a method for detecting MON 87708 by contacting a sample comprising DNA with a probe that, when used in a hybridization reaction with DNA from the MON 87708 soybean event, hybridizes to a DNA molecule specific to the MON 87708 soybean event, carrying out a hybridization reaction, and detecting the hybridization of the probe to the DNA molecule. Kits comprising the methods and compositions of the invention are also provided, useful for detecting the presence of DNA derived from the MON 87708 soybean event. The invention provides a soybean plant, seed, plant cell, progeny plant, plant part, or raw material derived from a plant, plant cell, or seed of the soybean event MON 87708. The invention further provides a soybean plant, seed, plant cell, progeny plant, plant part, or raw material comprising a recombinant DNA molecule having a nucleotide sequence selected from the group consisting of SEC ID NO: 1-8, and complements and fragments thereof. The invention further provides a soybean plant, seed, plant cell, progeny plant, plant part, or raw material derived from the plant or seed of the soybean event MON 87708 and comprising a recombinant DNA molecule that produces an amplified DNA molecule comprising SEC ID NO: 1, SEC ID NO: 2, SEC ID NO: 7, and / or SEC ID NO: 8 in a DNA amplification method. The invention provides a method for controlling weeds in a field by planting the MON 87708 soybean event and then applying an effective dose of the herbicide dicamba capable of controlling weeds without harming the MON 87708 soybean plants. The invention further provides a method for controlling weeds in a field by applying an effective dose of dicamba herbicide and then planting the MON 87708 soybean event in the field. The invention further provides a method for IF-2019-80266805-APN-ANP#INPI Page 4 of 50 to produce soybean seed essentially free of the seeds of toxic weed species by planting seeds of a dicamba-tolerant soybean variety MON 87708 in a field, applying an effective post-emergence dose of dicamba herbicide sufficient to kill the toxic weed species in the field, and collecting seed from the field. The invention provides methods for producing a soybean plant and / or seed tolerant to the application of the herbicide dicamba by sexually crossing a plant of the soybean event MON 87708 comprising SEC ID NO: 1, SEC ID NO: 2, SEC ID NO: 7, and / or SEC ID NO: 8 with a second soybean plant, thereby producing a seed, cultivating the seed to produce progeny plants, treating the progeny plants with dicamba, and selecting a progeny plant that is tolerant to dicamba. The methods may also include self-fertilizing the selected progeny plant to produce a plurality of second-generation progeny plants and selecting from among these a dicamba-tolerant plant.The methods may further include sexually crossing the selected progeny plant with another soybean plant to produce seed, cultivating the seed to produce a second generation of progeny plants, treating the second generation of progeny plants with dicamba, and selecting a second-generation progeny plant that is tolerant to dicamba. The invention provides methods for producing a soybean plant and / or seed that tolerates the application of the herbicide dicamba by self-fertilizing a plant of the dicamba-tolerant soybean event MON 87708 comprising SEC ID NO: 1, SEC ID NO: 2, SEC ID NO: 7, and / or SEC ID NO: 8, thereby producing seed, cultivating the seed to produce progeny plants, treating the progeny plants with dicamba, and selecting a progeny plant that is tolerant to dicamba. IF-2019-80266805-APN-ANP#INPI Page 5 of 50 The invention provides methods for determining the zygosity of a soybean plant of the MON 87708 event or seed comprising contacting a soybean DNA sample with a set of primers comprising SEC ID NO: 12, SEC ID NO: 13, and SEC ID NO: 14 and a set of probes comprising SEC ID NO: 15 and SEC ID NO: 16; then carrying out a nucleic acid amplification reaction with the sample, primer set, and probe set; then detecting in the nucleic acid amplification reaction a first fluorescent signal that is diagnostic for the MON 87708 event and a second fluorescent signal different from the first fluorescent signal and that is diagnostic for native soybean genomic DNA corresponding to the insertion location of the MON 87708 event transgene;and analyze the presence and / or absence of the first fluorescent signal and the second fluorescent signal in the nucleic acid amplification reaction, where the presence of both fluorescent signals indicates that the sample is heterozygous for the MON 87708 event and the presence of only the first fluorescent signal indicates that the sample is homozygous for the MON 87708 event.; The invention further provides a soybean plant, seed, plant cell, or plant part comprising the soybean haplotype region in linkage group 9 at approximately map position 143.5, comprising a dicamba tolerance gene and further defined by haplotype windows 19743 and 19767, and methods of using the same. The foregoing and other aspects of the invention will become more apparent from the following detailed description. BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 illustrates the organization of the transgenic insert in the genome of the MON 87708 soybean event; [A] corresponds to the relative position of SEC ID NO: 1, which is sixty nucleotides from the junction between the soybean genomic DNA and the IF-2019-80266805-APN-ANP#INPI Page 6 of 50 portion 5 of the DNA of the transgenic insert DNA; [A'] corresponds to the relative position of SEC ID NO: 7, which is one hundred nucleotides from the junction between the soybean genomic DNA and the 5' portion of the transgene insert DNA; [B] corresponds to the relative position of SEC ID NO: 2, which is sixty nucleotides from the junction between the soybean genomic DNA and the 3' portion of the transgene insert DNA; [B'] corresponds to the relative position of SEC ID NO: 8, which is one hundred nucleotides from the junction between the soybean genomic DNA and the 3' portion of the transgenic insert DNA; [C] corresponds to the relative position of SEC ID NO: 3, which is the soybean genomic sequence flanking the arbitrarily assigned / designated 5' end of the expression cassette integrated into the genome at event MON 87708;[D] corresponds to the relative position of SEC ID NO: 4, which is the soybean genomic sequence flanking the arbitrarily assigned / designated 3' end of the expression cassette integrated into the genome at event MON 87708; [E] represents the various elements comprising SEC ID NO: 5 and is the sequence of the expression cassette inserted into the genome at event MON 87708; and [F] represents the contiguous sequence (provided as SEC ID NO: 6) comprising, as depicted in the figure from left to right, SEC ID NO: 3, SEC ID NO: 5, and SEC ID NO: 4, wherein SEC ID NO: 1, SEC ID NO: 2, SEC ID NO: 7, and SEC ID NO: 8 are included, as these sequences are present in the genome at event MON 87708. BRIEF DESCRIPTION OF THE SEQUENCES SEC ID NO: 1 is a sixty-nucleotide sequence representing the 5' link between the soybean genomic DNA and the integrated transgenic expression cassette. SEC ID NO: 1 is positioned in SEC ID NO: 6 at nucleotide positions 1097-1156. IF-2019-80266805-APN-ANP#INPI Page 7 of 50 SEC ID NO: 2 is a sixty-nucleotide sequence representing the 3' junction between the soybean genomic DNA and the integrated transgenic expression cassette. SEC ID NO: 2 is positioned in SEC ID NO: 6 at nucleotide positions 4100-4159. SEC ID NO: 3 is the 5' sequence flanking the inserted DNA of the MON 87708 soybean event up to and including a transgenic DNA insertion region. SEC ID NO: 4 is the 3' sequence flanking the inserted DNA of the MON 87708 soybean event up to and including a transgenic DNA insertion region. SEC ID NO: 5 is the sequence of the integrated transgenic expression cassette. SEC ID NO: 6 is the nucleotide sequence representing the contig of the 5' sequence flanking the inserted DNA of the MON 87708 soybean event (SEC ID NO: 3), the inserted DNA sequence (SEC ID NO: 5), and the 3' sequence flanking the inserted DNA of the MON 87708 soybean event (SEC ID NO: 4) and includes SEC ID NO: 1, SEC ID NO: 2, SEC ID NO: 7, and SEC ID NO: 8. SEC ID NO: 7 is a sequence of one hundred nucleotides that represents the 5' junction between the soybean genomic DNA and the integrated transgenic expression cassette. SEC ID NO: 8 is a sequence of one hundred nucleotides that represents the 3' junction between the soybean genomic DNA and the integrated transgenic expression cassette. SEC ID NO: 9 is the sequence of a primer called Primer SQ13570 and used to identify the MON 87708 soybean event. It is complementary to the expression cassette inserted in the region near the 3' insertion edge of the transgene. A PCR amplicon produced from an assay IF-2019-80266805-APN-ANP#INPI Page 8 of 50 TAQMAN® (PE Applied Biosystems, Foster City, CA) using the combination of primers SQ13570 and SQ13571 (SEC ID NO: 10) is a positive result for the presence of event MON 87708. SEC ID NO: 10 is the sequence of a primer called Primer SQ13571 and used to identify the MON 87708 soybean event. It is complementary to a 3' region flanking the inserted expression cassette and close to the transgenic DNA insertion site. A PCR amplicon produced from a TAQMAN® assay (PE Applied Biosystems, Foster City, CA) using the combination of primers SQ13570 (SEC ID NO: 9) and SQ13571 yielded a positive result for the presence of the MON 87708 event. SEC ID NO: 11 is the sequence of a probe called Probe PB4655, used to identify the MON 87708 soybean event. It is complementary to a region spanning the 3' junction of the inserted expression cassette and the genomic DNA. This probe is a synthetic oligonucleotide labeled with 6-FAM™. The release of a fluorescent signal in an amplification reaction using primers SQ13570 and SQ13571 (SEC ID NO: 9-10) in combination with the 6-FAM™-labeled probe PB4655 is diagnostic of the MON 87708 event in a TAQMAN® assay. SEC ID NO: 12 is the sequence of a primer called Primer SQ20632 and used to identify the zygosity of event MON 87708. SEC ID NO: 13 is the sequence of a primer called Primer SQ20636 and is used to identify the zygosity of the MON 87708 event and wild type of soybean. SEC ID NO: 14 is the sequence of a primer called Primer SQ20637 and is used to identify the zygosity of the wild type of soybean. SEC ID NO: 15 is the sequence of a probe called Probe PB10130 and is used for a zygosity assay of event MON 87708. IF-2019-80266805-APN-ANP#INPI Page 9 of 50 SEC ID NO: 16 is the sequence of a probe called Probe PB10131 and is used for a wild-type soybean zygosity assay. DETAILED DESCRIPTION The following definitions and methods are provided for a better definition of the invention and to guide those skilled in the art in the practice of the invention. Unless otherwise indicated, the terms should be understood in accordance with conventional usage by those skilled in the relevant art. The invention provides a transgenic soybean event, MON 87708, that exhibits commercially acceptable tolerance to dicamba herbicide applications. The event comprises a single insertion of transgenic DNA into the chromosome / genome of the soybean germplasm. An “event” is produced by: (i) transformation of a plant cell with a nucleic acid construct that includes a transgene of interest, (ii) regeneration of a plant population resulting from the insertion of the transgene into the plant genome, and (iii) selection of a particular plant characterized by the insertion of the transgene at a particular location in the plant genome. The term “event” refers to the original transformant that includes the inserted transgene at the particular location in the plant genome. The term “event” further refers to the progeny of the transformant that includes the inserted transgene at the particular location in the plant genome.Such progeny can be produced by sexual outcrossing between the transformant, or its progeny, and another plant. This other plant can be a transgenic plant comprising the same transgene or a different transgene and / or a non-transgenic plant, such as one of a different variety. Even after repeated backcrossing to a recurrent parent, the inserted DNA and flanking DNA of the transformed parent are present in the cross progeny at the same genomic location. IF-2019-80266805-APN-ANP#INPI Page 10 of 50 As used in this invention, the term “soybean” means Glycine max and includes all varieties of plants that can be cultivated with soybeans, including wild soybean species as well as those plants belonging to Glycine that allow cross-species cultivation. The term “event” further refers to a DNA molecule of the original transformant comprising the inserted DNA and the flanking soybean genomic DNA immediately adjacent to either side of the inserted DNA. This DNA molecule is created by the act of inserting the transgenic DNA into the soybean plant genome, i.e., by the act of transformation. Therefore, this DNA molecule comprises a nucleotide sequence that is both specific to the event and unique to the soybean plant genome into which the transgenic DNA has been inserted, in that this nucleotide sequence contains the sequence of both a particular region of soybean genomic DNA and the transgenic DNA insert. The arrangement of the inserted DNA in the MON 87708 soybean event relative to the surrounding soybean plant genomic DNA is thus specific to and unique to the MON 87708 soybean event.This DNA molecule is also an integral part of the soybean chromosome of the MON 87708 event and, as such, is static in the plant and can be passed on to the plant's progeny. The MON 87708 event comprises a transgene that confers tolerance to dicamba herbicide applications in soybeans. “Dicamba” refers to 3,6-dichloro-2-methoxybenzoic acid. Dicamba is a synthetic auxin herbicide useful for controlling broadleaf weeds. Soybean plants were transformed with dicamba monooxygenase (DMO), an enzyme cloned from Stenotrophomonas maltophilia, which is commonly found in the soil rhizosphere. Dicamba monooxygenase is an enzyme that catalyzes the deactivation of dicamba through an O-demethylation reaction to the non-herbicidal compound 3,5-dichloro-2-methoxybenzoic acid. Page 11 of 50 Dichlorosalicylic acid. In some areas of the world, seeds from toxic weed species can contaminate harvested soybean seeds, potentially affecting the health and nutrition of animals fed the contaminated soybean commodities. These plants can be eliminated from a soybean field by treatment with the herbicide dicamba. Members of this group of toxic weeds include Cardaria spp., Heliotropium spp., Centaurea spp., Senecio spp., Crotalaria spp., Solanum spp., Xanthium spp., Amsinckia spp., Cassia spp., Sesbania spp., Datura spp., Ricinus spp., Argemone spp., Corchorus spp., Impomoea spp., and Echium spp. As used in this invention, the term “recombinant” refers to a form of DNA and / or protein and / or an organism that would normally be found in nature and as such was created by human intervention. Such human intervention can produce a recombinant DNA molecule and / or a recombinant plant. As used in this invention, a “recombinant DNA molecule” is a DNA molecule comprising a combination of DNA molecules that would occur naturally together and is the result of human intervention, e.g.A DNA molecule composed of a combination of at least two heterologous DNA molecules, and / or a DNA molecule that is artificially synthesized and comprises a polynucleotide sequence that deviates from the polynucleotide sequence that would normally exist in nature, and / or a DNA molecule comprising a transgene artificially incorporated into a host cell's genomic DNA and the associated flanking DNA of the host cell's genome. An example of a recombinant DNA molecule is a DNA molecule described in this invention that results from the insertion of the transgene into the genomic DNA of soybeans, which may ultimately result in the expression of a recombinant RNA and / or protein molecule in that organism. IF-2019-80266805-APN-ANP#INPI Page 12 of 50 As employed in this invention, a “recombinant plant” is a plant that would normally exist in nature, is the result of human intervention, and contains a transgene and / or heterologous DNA molecule incorporated into its genome. As a result of such genomic alteration, the recombinant plant is distinctly different from the related wild-type plant. An example of a recombinant plant is a soybean plant described in this invention as Event MON 87708. As used in this invention, the term “transgene” refers to a nucleotide molecule artificially incorporated into the genome of a host cell. Such a transgene may be heterologous to the host cell. The term “transgenic plant” refers to a plant comprising such a transgene. As used in this invention, the term “heterologous” refers to a first molecule not normally found in combination with a second molecule in nature. For example, a molecule may be derived from a first species and inserted into the genome of a second species. The molecule would therefore be heterologous to the host and artificially incorporated into the host cell's genome. As used in this invention, the term “chimeric” refers to a single DNA molecule produced by fusing a first DNA molecule to a second DNA molecule, where neither the first nor the second DNA molecule would normally be found in that configuration, i.e., fused to each other. Therefore, the chimeric DNA molecule is a novel DNA molecule not otherwise normally found in nature. The invention provides DNA molecules and their corresponding nucleotide sequences. As used in this invention, the term “DNA”, IF-2019-80266805-APN-ANP#INPI Page 13 of 50 “DNA molecule” and “nucleotide molecule” refer to a DNA molecule of genomic or synthetic origin, i.e., a polymer of deoxyribonucleotide bases or a polynucleotide molecule, read from the 5' (upstream) end to the 3' (downstream) end. As used in this invention, the term “DNA sequence,” “nucleotide sequence,” or “polynucleotide sequence” refers to the nucleotide sequence of a DNA molecule. The nomenclature employed in this invention is that required by Title 37 of the United States Code of Federal Regulations § 1822 and set forth in the tables in WIPO Standard ST.25 (1998), Appendix 2, Tables 1 and 3. By convention, the nucleotide sequences of the invention provided as SEC ID NO: 1-8 and their fragments are described with reference to only one strand of the two strands. of complementary nucleotide sequences.By implication, complementary sequences (i.e., complementary strand sequences), also referred to in the art as inverse complementary sequences, are within the scope of the invention and are expressly intended to be within the scope of the claimed object. The nucleotide sequence corresponding to the complete nucleotide sequence of the inserted transgenic DNA and substantial segments of the soybean genomic DNA flanking either end of the inserted transgenic DNA is provided in the present invention as SEC ID NO: 6. A subsection thereof is the inserted transgenic DNA provided as SEC ID NO: 5. The nucleotide sequence of the soybean genomic DNA physically linked by phosphodiester bond ligation and therefore flanking the 5' end of the inserted transgenic DNA is exposed as shown in SEC ID NO: 3. The nucleotide sequence of the soybean genomic DNA physically linked by IF-2019-80266805-APN-ANP#INPI Page 14 of 50 phosphodiester bond ligation to, and therefore, flanking the 3' end of the inserted transgenic DNA is exposed as shown in SEC ID NO: 4. The MON 87708 soybean event further comprises two regions, one spanning the 5' location and the other spanning the 3' location, where the transgenic DNA is inserted into the genomic DNA, referred to in this invention as the 5' and 3' junctions, respectively. A "junction sequence" or "junction region" refers to the corresponding DNA sequence and / or DNA molecule spanning the inserted transgenic DNA and the adjacent flanking genomic DNA. The junction sequences may be arbitrarily represented by the two 60 nucleotide sequences provided as SEC ID NO: 1 and SEC ID NO: 2, each representing 30 nucleotides of the genomic DNA flanking, and contiguous with, 30 nucleotides of the inserted DNA.Alternatively, the joining sequences can be arbitrarily represented by the two 100-nucleotide sequences provided as SEC ID NO: 7 and SEC ID NO: 8, each representing 15 nucleotides of the flanking genomic DNA adjacent to, and contiguous with, 50 nucleotides of inserted DNA. These nucleotides are connected by phosphodiester ligation and, in the soybean event MON 87708, are present as part of the genome. In soybean, the identification of one or more of SEC ID NO: 1, SEC ID NO: 2, and SEC ID NO: 3, indicates that the sequences are linked by phosphodiester bonds and are present as part of the genome. 2, SEC ID NO: 7, and SEC ID NO: 8 in a sample derived from a soybean plant, seed, or plant part is determinative that the DNA was obtained from the soybean event MON 87708 and is diagnostic for the presence in a DNA sample of the soybean event MON 87708. The invention therefore provides a DNA molecule containing at least the nucleotide sequence as set forth in SEC ID NO: 1, SEC ID NO: 2, SEC ID NO: 7, 25, and / or SEC ID NO: 8.Any DNA segment derived from the transgenic soybean event MON 87708 that is sufficient to include SEC ID NO: 1, SEC ID NO: 2, SEC ID NO: 7, and / or SEC ID NO: 8 is within the scope of the. IF-2019-80266805-APN-ANP#INPI Page 15 of 50 Invention. Additionally, any polynucleotide comprising a sequence complementary to any of the sequences described in this paragraph is within the scope of the invention. Figure 1 illustrates the physical arrangement of SEC ID NO: 1-5 and 7-8 with respect to SEC ID NO. 6 arranged from 5' to 3'. The invention provides exemplary DNA molecules that can be used as either primers or probes to diagnose the presence of DNA derived from the MON 87708 soybean plant event in a sample. These primers or probes are specific for a target nucleic acid sequence and, as such, are useful for identifying the MON 87708 soybean event nucleic acid sequence using the methods of the invention described herein. A “primer” is typically an isolated, highly purified polynucleotide designated for use in specific hybridization and pairing methods involving thermal amplification. A pair of primers with template DNA, such as a soybean genomic DNA sample, can be used in a thermal amplification, such as polymerase chain reaction (PCR), to produce an amplicon. The amplicon produced from this reaction has a DNA sequence corresponding to the template DNA sequence located between the two sites where the primers hybridized to the template. As employed in this invention, an “amplicon” is a piece or fragment of DNA that has been synthesized using amplification techniques. An amplicon of the invention comprises at least SEC ID NO: 1, SEC ID NO: 2, SEC ID NO: 7, and / or SEC ID NO: 8.A primer is typically designed to hybridize to a complementary target DNA strand to form a primer-target DNA hybrid, and the presence of the primer is a recognition point by a polymerase to begin primer extension (i.e., polymerization of additional nucleotides into a molecule of). IF-2019-80266805-APN-ANP#INPI Page 16 of 50 elongation nucleotides) using the target DNA strand as a template. Primer pairs, as used in the invention, refer to the use of two primers that link opposite strands of a double-stranded nucleotide segment for the purpose of linearly amplifying the polynucleotide segment between the positions sought for binding by the individual members of the primer pair, typically in a thermal amplification reaction or other conventional nucleic acid amplification methods. Sample DNA molecules suitable as primers are provided as SEC ID NO: 9-10.The pair of primers provided as SEC ID NO: 9 and SEC ID NO: 10 are useful as a first DNA molecule and a second DNA molecule that is different from the first DNA molecule, and both are, individually, of sufficient length of contiguous nucleotides of SEC ID NO: 4, SEC ID NO: 5, or SEC ID NO: 6 to function as DNA primers that, when employed together in a thermal amplification reaction with template DNA derived from the soybean event MON 87708, produce an amplicon comprising SEC ID NO: 2. A “probe” is an isolated nucleic acid that is complementary to one strand of a target nucleic acid. Probes according to the invention include not only deoxyribonucleic or ribonucleic acids but also polyamides and other probe materials that bind specifically to a target DNA sequence, and the detection of such binding can be useful for diagnosing, discriminating, determining, or confirming the presence of that target DNA sequence in a particular sample. A probe can be bound to a conventional detectable reporter molecule or tag, e.g., a radioactive isotope, ligand, chemiluminescent agent, or enzyme. A sample DNA molecule useful as a probe is provided as SEC ID NO: 11. IF-2019-80266805-APN-ANP#INPI Page 17 of 50 The probes and primers according to the invention may have complete sequence identity with the target sequence, although primers and probes that differ from the target sequence but retain the ability to preferentially hybridize to target sequences may be designed using conventional methods. For a nucleic acid molecule to serve as a primer or probe, it must be sufficiently complementary in sequence to form a stable double-stranded structure under the particular solvent and salt concentrations employed. Any conventional nucleic acid hybridization or amplification method may be used to identify the presence of transgenic DNA of the MON 87708 soybean event in a sample.The probes and primers are generally at least approximately 11 nucleotides, at least approximately 18 nucleotides, at least approximately 24 nucleotides, or at least approximately 30 nucleotides or more in length. Such probes and primers hybridize specifically to a target DNA sequence under severe hybridization conditions. Conventional severity conditions are described by Sambrook et al., 1989, and by Haymes et al., in: Nucleic Acid Hybridization, A Practical Approach, IRL Press, Washington, DC (1985). As employed in this invention, two nucleic acid molecules are capable of specifically hybridizing to one another if the two molecules are capable of forming an antiparallel, double-stranded nucleic acid structure. A nucleic acid molecule is the “complement” of another nucleic acid molecule if they exhibit complete complementarity.As employed in this invention, molecules exhibit “complete complementarity” when each nucleotide of one molecule is complementary to a nucleotide of the other. Two molecules are “minimally complementary” if they can hybridize to each other with sufficient stability to allow them to remain paired to each other under stress. IF-2019-80266805-APN-ANP#INPI Page 18 of 50 less conventional “low severity” conditions. Similarly, molecules are “complementary” if they can hybridize with each other with sufficient stability to allow them to remain paired with one another under conventional “high severity” conditions. Deviations from complete complementarity are therefore permissible, as long as such deviations do not completely preclude the molecules’ ability to form a double-stranded structure. As used in this invention, the term “isolated” refers to at least partially separating a molecule from other molecules normally associated with it in its native or natural state. In one embodiment, the term “isolated” refers to a DNA molecule that is at least partially separated from the nucleic acids that normally flank the DNA molecule in its native or natural state. Therefore, DNA molecules fused to regulatory or coding sequences with which they are not normally associated, for example, as a result of recombinant techniques, are considered isolated in this invention. Such molecules are considered isolated even when integrated into the chromosome of a host cell or present in a nucleic acid solution with other DNA molecules. Any number of methods well known to those skilled in the art may be used to isolate and manipulate a DNA molecule, or a fragment thereof, described in the invention. For example, PCR (polymerase chain reaction) technology may be used to amplify a particular starting DNA molecule and / or to produce variants of the original molecule. DNA molecules or fragments thereof may also be obtained by other techniques, such as directly synthesizing the fragment by chemical means, as is common practice using an automated oligonucleotide synthesizer. IF-2019-80266805-APN-ANP#INPI Page 19 of 50 The DNA molecules and corresponding nucleotide sequences provided in this invention are therefore useful for, among other things, identifying the MON 87708 soybean event, selecting plant varieties or hybrids comprising the MON 87708 soybean event, detecting the presence of DNA derived from the MON 87708 transgenic soybean event in a sample, and monitoring samples to determine the presence and / or absence of the MON 87708 soybean event or plant parts derived from the MON 87708 soybean event. The invention provides soybean plants, progeny, seeds, plant cells, plant parts (such as pollen, ovule, pod, flower tissue, root tissue, stem tissue, and leaf tissue), and commodities. These plants, progeny, seeds, plant cells, plant parts, and commodities contain a detectable amount of a polynucleotide of the invention, i.e., a polynucleotide having at least one of the sequences provided as SEC ID NO: 1-8. The plants, progeny, seeds, plant cells, and plant parts of the invention may also contain one or more additional transgenes.Such a transgene can be any nucleotide sequence that codes for a protein or RNA molecule that confers a desirable characteristic including, but not limited to, increased insect resistance, increased water use efficiency, increased yield, increased drought resistance, higher seed quality, improved nutritional quality, and / or increased herbicide tolerance, where the desirable characteristic is measured against a soybean plant lacking such additional transgene. The invention provides soybean plants, progeny, seeds, plant cells, and plant parts such as pollen, ovule, pod, flower, root or stem tissue, and leaves derived from a transgenic soybean plant event MON 87708. A representative seed sample of the MON 87708 soybean event has been deposited in accordance with the Budapest Treaty for the purpose of making IF-2019-80266805-APN-ANP#INPI Page 20 of 50 The invention is possible. The repository selected to receive the deposit is the American Type Culture Collection (ATCC), which has an address at 10801 University Boulevard, Manassas, Virginia, USA, Zip Code 20110. The ATCC repository has assigned accession number PTA-9670 to the seed with event MON 87708. The invention provides a microorganism comprising a DNA molecule having SEC ID NO: 1 and SEC ID NO: 2 present in its genome. An example of such a microorganism is a transgenic plant cell. Microorganisms, such as a plant cell of the invention, are useful in many industrial applications, including but not limited to: (i) use as a research tool for scientific research or industrial investigation; (ii) use in culture to produce endogenous or recombinant carbohydrate, lipid, nucleic acid, or protein products or small molecules that can be employed for further scientific research or as industrial products; and (iii) use with modern plant tissue culture techniques to produce transgenic plants or plant tissue cultures that can then be employed for agricultural research or production.The production and use of microorganisms such as transgenic plant cells utilizes modern microbiological techniques and human intervention to produce a unique, man-made microorganism. In this process, recombinant DNA is inserted into a plant cell genome to create a transgenic plant cell that is distinct from and unique to natural plant cells. This transgenic plant cell can then be cultured in a manner much like bacterial and yeast cells using modern microbiological techniques and can exist in a unicellular, undifferentiated state. The new genetic makeup of the plant cell and its phenotype are a IF-2019-80266805-APN-ANP#INPI Page 21 of 50 The technical effect created by the integration of heterologous DNA into the cell's genome. Another aspect of the invention is a method of using a microorganism of the invention. The methods of using microorganisms of the invention, such as transgenic plant cells, include (i) methods for producing transgenic cells by integrating recombinant DNA into the cell's genome and then using this cell to derive additional cells possessing the same heterologous DNA; (ii) methods of culturing cells containing recombinant DNA using modern microbiology techniques; (iii) methods for producing and purifying endogenous or recombinant carbohydrate, lipid, nucleic acid, or protein products from cultured cells; and (iv) methods of using modern plant tissue culture techniques with transgenic plant cells to produce transgenic plants or transgenic plant tissue cultures. The plants of the invention can pass the event DNA, including the transgene, to their offspring. As used in this invention, “offspring” includes any plant, seed, plant cell, and / or regenerable plant part comprising the event DNA derived from an ancestral plant and / or a polynucleotide having at least one of the sequences provided as SEC ID NO: 1 and SEC ID NO: 2. The plants, offspring, and seeds can be homozygous or heterozygous for the transgene. The offspring can be grown from seeds produced by a plant with the MON 87708 soybean event and / or from seeds produced by a plant fertilized with pollen from a plant with the MON 87708 soybean event. The progeny plants can be self-pollinated (also known as “selfing”) to generate a true genetic improvement line of plants, that is, plants homozygous for the transgene. IF-2019-80266805-APN-ANP#INPI Page 22 of 50 Self-fertilization of appropriate progeny can produce plants that are homozygous for both added exogenous genes. Alternatively, the progeny plants may be crossed by outcrossing, e.g., crossed with another unrelated plant, to produce a varietal or hybrid seed or plant. The other unrelated plant may be transgenic or non-transgenic. A varietal or hybrid seed or plant of the invention may, therefore, be derived by crossing a first parent lacking the specific and unique DNA of the MON 87708 soybean event with a second parent comprising the MON 87708 soybean event, resulting in a hybrid comprising the specific and unique DNA of the MON 87708 soybean event. Each parent may be a hybrid or an inbred / varietal, provided that the crossing or reproduction results in a plant or seed of the invention, i.e., a seed having at least one allele containing the unique and specific DNA of the MON 87708 soybean event and / or SEC ID NO: 1 and SEC ID NO: 2.Therefore, two different transgenic plants can be crossed to produce hybrid offspring containing two exogenous, independently assimilated genes. For example, the dicamba-tolerant soybean MON 87708 can be crossed with another transgenic soybean plant to produce a plant that has the characteristics of both transgenic parents. An example of this would be a cross of the dicamba-tolerant soybean MON 87708 with a plant that has one or more additional characteristics such as herbicide tolerance (e.g., soybean event 40-3-2 or soybean event MON89788 (U.S. Patent Application Publication No. 20060282915)), insect control (e.g., soybean event MON87701 (U.S. Patent Application Publication No. 20090130071)), and / or other desirable characteristics (e.g., higher oil composition such as soybean event MON87769 (PCT Patent Publication). IF-2019-80266805-APN-ANP#INPI Page 23 of 50 W02009102873)), resulting in a progeny plant or seed that is tolerant to dicamba and has one or more additional characteristics. Herbicides for which tolerance of transgenic plants has been demonstrated and for which the method of the invention can be applied include, but are not limited to: glyphosate, glufosinate, sulfonylureas, imidazolinones, bromoxynil, delapon, cyclohexanedione, protoporphyrinogen oxidase inhibitors, and isoxasflutol herbicides. Nucleotide molecules that encode proteins involved in herbicide tolerance are known in the art and include, but are not limited to, a nucleotide molecule that encodes: glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) (see, for example, U.S. Patent Nos. 5,627,061; 5,633,435; 6,040,497; 5,094,945; 5,804,425; 6,248,876; 7,183,110; RE39,247); glyphosate oxidoreductase (GOX) (see, for example, U.S. Patent No. 5,776).760); glyphosate-n-acetyltransferase (GAT); a herbicide-tolerant acetolactate synthase (ALS, also known as acetohydroxy acid synthase (AHAS)) for tolerance to sulfonylureas, imidazolinones, triazolopyrimidines, pyrimidinyloxybenzoates, sulfonylamino carbonyl triazolinones, and / or heteroaryl ethers; a herbicide-tolerant acetyl coenzyme A carboxylase (ACCase) or R-2,4-dichlorophenoxypropionate dioxygenase (rdpA) for tolerance to an aryloxyphenoxypropionate (AOPP) (such as haloxyfop, quizalofop, dichlorofop, and diclofop); a detoxification protein such as a 2,4-D dioxygenase (tfdA), R-2,4-dichlorophenoxypropionate dioxygenase (rdpA), AryloxyAlkanoate Dioxygenase (AAD), and / or S-2,4-dichloroprop dioxygenase (sdpA) for tolerance to synthetic auxin herbicides; a bromoxynyl nitrilase (Bxn) for tolerance to Bromoxynyl (see, for example, U.S. Patent No. 4,810).648); a phytoene desaturase (crtl) for norflurazon tolerance; biafos resistance (bar) or phosphinothricin acetyltransferase (PAT) protein (see, for example, U.S. Patent Nos. 5,646,024 and 5,276,268) for glufosinate tolerance. IF-2019-80266805-APN-ANP#INPI Page 24 of 50 and bialofos; and a triketone herbicide tolerance protein (mezotrione, tembotrione, topromezone, isoxazole) such as tolerant to 4-HydroxyPhenylPyruvate Dioxygenase (HPPD), a cytochrome P450 detoxifying agent, or a HPPD pathway divert such as Artbrobacter globiformis HPP oxidase (HPPO) and Pseudomonas acidovorans 4-HPA 1-hydroxylase (HPAH) and NADH oxidoreductase (HPAC). Backcrossing to a parental plant and outcrossing with a non-transgenic plant are also considered, as is vegetative propagation. Descriptions of other breeding methods commonly used for different traits and crops can be found in various references, e.g., Fehr, in Breeding Methods for Cultivar Development, Wilcox J. ed., American Society of Agronomy, Madison Wl (1987). The invention provides a plant part derived from the MON 87708 soybean event. As used herein, a “plant part” refers to any part of a plant composed of material derived from a plant with the MON 87708 soybean event. Plant parts include, but are not limited to, pollen, ovule, pod, flower, root or stem tissue, fibers, and leaves. Plant parts may be viable, non-viable, regenerable, and / or non-regenerable. The invention provides a commodity derived from the MON 87708 soybean event. As used herein, a “commodity” refers to any composition or product comprised of material derived from a plant with the MON 87708 soybean event, including seed, plant cells, or plant parts. Commodities may be sold to consumers and may be viable or non-viable. Non-viable commodities include, but are not limited to: non-viable seeds and grains; processed seeds, seed parts, and plant parts; and dehydrated plant tissue. IF-2019-80266805-APN-ANP#INPI Page 25 of 50 Frozen and processed plant tissue; seeds and processed plant parts for animal feed for terrestrial and / or aquatic animals, oil, milled grain, flour, flakes, bran, fiber, milk, cheese, paper, cream, wine, and any other food for human consumption; and biomass and fuel products. Viable commodities include, but are not limited to, seeds and plant cells. The MON 87708 soybean event may, therefore, be used to manufacture any commodity typically acquired from soybeans. Any such commodity derived from the MON 87708 soybean event may contain at least a detectable amount of the specific and unique DNA corresponding to the MON 87708 soybean event, and may specifically contain a detectable amount of a polynucleotide containing at least 15 contiguous nucleotides of SEC ID NO: 1 or SEO ID NO: 2.Any conventional detection method for nucleotide molecules may be used, including detection methods described in this invention. A raw material is within the scope of the invention if any detectable amount of SEC ID NO: 1 or SEC ID NO: 2 is present in the raw material. The plants, progeny, seeds, plant cells, plant parts (such as pollen, ovule, pod, flower, root or stem tissue and leaves), and basic products of the invention are, therefore, useful for, among other things, cultivating plants for the purpose of producing seed and / or plant parts of the MON 87708 soybean event for agricultural purposes, producing progeny of the MON 87708 soybean event for plant breeding and research purposes, use with microbiological techniques for industrial and research applications, and sale to consumers. The invention provides methods for controlling weeds and methods for producing plants using dicamba herbicide and the MON 87708 soybean event. IF-2019-80266805-APN-ANP#INPI Page 26 of 50 One method for weed control in a field is provided and consists of planting varietal or hybrid plants with the MON 87708 soybean trait in a field and applying an effective herbicidal dose of dicamba to the field for weed control without harming the MON 87708 plants. This dicamba herbicide application can be pre-emergence, meaning at any time after the MON 87708 seed is planted and before the MON 87708 plants emerge, or post-emergence, meaning at any time after the MON 87708 plants emerge. Another method for weed control in a field is also provided and consists of applying an effective dose of dicamba herbicide to control weeds in a field and then planting the MON 87708 soybean trait in the field.This type of dicamba herbicide application would be pre-planted, i.e., before the MON 87708 seed is planted, and could be carried out at any time prior to planting, including, but not limited to, approximately 14 days pre-plant to approximately 1 day prior to planting. The invention further provides a method for producing soybean seed essentially free of the seeds of toxic weed species by planting seeds of a dicamba-tolerant soybean variety MON 87708 in a field, applying an effective post-emergence dose of dicamba herbicide sufficient to kill the toxic weed species in the field, and harvesting the seed from the field. An effective herbicide dose of dicamba for use in the field should consist of a range between approximately 0.0056 kilograms per hectare (0.005 pounds per acre) and approximately 8.96 kilograms per hectare (8 pounds of dicamba per acre) during a growing season.Multiple applications of dicamba can be used during one growing season, for example, two applications (such as a pre-plant application and a post-emergence application or a pre-emergence application and a post-emergence application). IF-2019-80266805-APN-ANP#INPI Page 27 of 50 or three applications (such as a pre-planted application, a pre-emergence application, and a post-emergence application). Methods are provided for producing a herbicide-tolerant soybean plant comprising specific and unique DNA sequences for the transgenic event MON 87708 of the invention. The transgenic plants employed in these methods may be homozygous or heterozygous for the transgene. The progeny plants produced by these methods may be varietal or hybrid plants; they may be grown from seeds produced by a plant with the soybean event MON 87708 and / or from seeds produced by a plant fertilized with pollen from a plant with the soybean event MON 87708; and they may be homozygous or heterozygous for the transgene. The progeny plants may be subsequently self-pollinated to generate a true breeding line of plants, i.e., plants homozygous for the transgene, or alternatively, they may be outcrossed, e.g., reproduced with another unrelated plant, to produce a plant variety or seed or a hybrid. A soybean plant tolerant to the herbicide dicamba can be produced by sexually crossing a plant with the MON 87708 event, which comprises a nucleotide molecule containing the sequences SEC ID NO: 1 and SEC ID NO: 2, with another soybean plant, thereby producing seed that is then grown into progeny plants. These progeny plants can then be treated with the herbicide dicamba to select progeny plants that are tolerant to the herbicide dicamba. Alternatively, these progeny plants can be analyzed using diagnostic methods to select progeny plants that contain the DNA of the MON 87708 event. The other plant used in the cross may or may not be tolerant to the herbicide dicamba and may or may not be transgenic. The progeny plant and / or seed produced may be a variety of IF-2019-80266805-APN-ANP#INPI Page 28 of 50 seed or hybrid seed. In the practice of this method, the step of sexually crossing one plant with another plant, that is, cross-pollination, can be achieved or facilitated by human intervention, for example: by human hands collecting pollen from one plant and bringing this pollen into contact with the style or stigma of a second plant; by human hands and / or actions that remove, destroy, or cover the stamen or anthers of a plant (e.g., by plucking or by applying a chemical gametocide) so as to prevent natural self-pollination, and cross-pollination would have to be carried out in order for fertilization to occur; by human placement of pollinating insects in a position for “directed pollination” (e.g.by placing beehives in orchards or fields or caging plants with pollinating insects); by human opening or removal of parts of the flower to allow the placement or contact of foreign pollen on the style or stamen (e.g., in soybeans that naturally have flowers that prevent or avoid cross-pollination, making them naturally obligate self-pollinators without human intervention); by selective placement of plants (e.g., intentionally planting plants in proximity to pollination); and / or by applying chemical products to precipitate flowering or to promote receptivity (of the stigma to pollen). A soybean plant tolerant to the herbicide dicamba can be produced by self-fertilizing a plant with the MON 87708 event, which comprises a nucleotide molecule with the sequences SEC ID NO: 1 and SEC ID NO: 2, thereby producing seed that is then grown into progeny plants. These progeny plants can then be treated with dicamba to select progeny plants that are tolerant to dicamba. Alternatively, these progeny plants can be analyzed using diagnostic methods to select progeny plants. IF-2019-80266805-APN-ANP#INPI Page 29 of 50 containing the DNA of the MON 87708 event. By implementing this method, the step of sexually crossing a plant with itself, i.e., self-pollination or self-fertilization, can be achieved or facilitated by human intervention, for example: by human hands collecting pollen from the plant and bringing this pollen into contact with the style or stigma of the same plant and then optionally preventing further fertilization of the plant; by human hands and / or actions that remove, destroy, or cover the stamen or anthers of other nearby plants (e.g., by plucking or by applying a chemical gametocide) so that cross-pollination is prevented and self-pollination would have to occur in order for fertilization to take place; by human placement of pollinating insects in a position for “directed pollination” (e.g.by caging a single plant with pollinating insects); by human manipulation of the flower or its parts to allow self-pollination; by selective placement of plants (e.g., intentionally planting plants beyond the proximity of pollination); and / or by applying chemicals to precipitate flowering or to promote receptivity (of the stigma to pollen). The progeny soybean plants or seeds covered by and produced using these methods will be distinct from other soybean plants, for example, because the progeny soybean plants and seeds: are recombinant and as such created by human intervention; are tolerant to the herbicide dicamba; contain at least one allele consisting of the transgenic DNA of the invention; and / or contain a detectable amount of a polynucleotide sequence selected from the group formed by SEC ID NO: 1 and SEC ID NO: 2. A seed may be selected from an individual progeny plant, and as long as the seed comprises SEC ID NO: 1 and SEC ID NO: 2, it will be within the scope of the invention. IF-2019-80266805-APN-ANP#INPI Page 30 of 50 By implementing the invention, two different transgenic plants can be crossed to produce hybrid offspring containing two independently segregating heterologous genes. Self-fertilization of suitable progeny can produce plants that are homozygous for both genes. Backcrossing to a parent plant and outcrossing to a non-transgenic plant are also contemplated, as is vegetative propagation. Descriptions of other methods commonly used for different traits and crops can be found in various references, e.g., Fehr, in Breeding Methods for Cultivar Development, Wilcox J. ed., American Society of Agronomy, Madison W1 (1987). The plants and seeds used in the methods described in this invention may also contain one or more additional transgenes. Such a transgene may be any nucleotide sequence encoding a protein or RNA molecule that confers a desirable characteristic, including, but not limited to, increased insect resistance, increased water use efficiency, increased yield, increased drought resistance, improved seed quality, enhanced nutritional quality, and / or increased herbicide tolerance, wherein the desirable characteristic is measured against a soybean plant lacking such additional transgene. The methods of the invention are therefore useful for, among other things, controlling weeds in a field while growing plants for the purpose of producing seed and / or plant parts of the MON 87708 soybean event for agricultural or research purposes, selecting progeny of the MON 87708 soybean event for plant breeding or research purposes, and producing progeny plants and seeds of the MON 87708 soybean event. Plants, progeny, seeds, plant cells, plant parts (such as pollen, ovule, pod, flower, root or stem tissue and leaves), and commodities IF-2019-80266805-APN-ANP#INPI Page 31 of 50 The components of the invention can be evaluated to determine DNA composition, gene expression, and / or protein expression. This type of evaluation can be performed using any conventional method such as PCR, northern blotting, southern blotting, western blotting, immunoprecipitation, and ELISA, or using the detection methods and / or detection kits provided in this invention. Methods are provided for detecting the presence of DNA derived from a soybean cell, tissue, seed, or plant of the MON 87708 soybean event in a sample. One method consists of (i) extracting a DNA sample from at least one soybean cell, tissue, seed, or plant, (ii) contacting the DNA sample with a primer pair capable of producing an amplicon of the MON 87708 event DNA under conditions appropriate for DNA amplification, (iii) carrying out a DNA amplification reaction, and then (iv) detecting the amplicon molecule and / or confirming that the nucleotide sequence of the amplicon comprises a nucleotide sequence specific to the MON 87708 event, such as one selected from the group consisting of SEC ID NO: 1-8. The amplicon should be one that is specific to the MON 87708 event, such as an amplicon comprising SEC ID NO: 1 or SEC ID NO: 2.The detection of a nucleotide sequence specific to the MON 87708 event in the amplicon is conclusive and / or diagnostic of the presence of DNA specific to the MON 87708 soybean event in the sample. An example of a primer pair capable of producing an amplicon from the MON 87708 event DNA under appropriate DNA amplification conditions is provided as SEC ID NO: IOI 1. Other primer pairs can be readily designed by a person skilled in the art and would comprise at least one fragment of SEC ID NO: 6. Another method for detecting the presence of DNA derived from a cell, tissue, seed, or plant of the MON 87708 soybean event in a sample consists of (i). IF-2019-80266805-APN-ANP#INPI Page 32 of 50 Extract a DNA sample from at least one soybean cell, tissue, seed, or plant; (i) contact the DNA sample with a DNA probe specific for the MON 87708 event DNA; (iii) allow the probe and DNA sample to hybridize under severe hybridization conditions; and then (iv) detect the hybridization between the probe and the target DNA sample. An example of the sequence of a DNA probe that is specific for the MON 87708 event DNA is provided as SEC ID NO: 11. Other probes can be readily designed by a person skilled in the art and would comprise at least a fragment of SEC ID NO: 6. Detection of hybridization of the probe to the DNA sample diagnoses the presence of the MON 87708 soybean event-specific DNA in the sample. The absence of hybridization, alternatively, diagnoses the absence of the MON 87708 soybean event-specific DNA in the sample. DNA detection kits are provided that are useful for identifying DNA from the MON 87708 soybean event in a sample and can also be applied to methods for the genetic improvement of soybean plants containing the DNA of the appropriate event. These kits contain primers and / or DNA probes comprising fragments of SEC ID NO: 1-8. An example of such a kit comprises at least one DNA molecule of sufficient length of contiguous nucleotides of SEC ID NO: 6 to function as a DNA probe useful for detecting the presence and / or absence of DNA derived from the MON 87708 transgenic soybean event in a sample. DNA derived from the MON 87708 transgenic soybean event would comprise SEC ID NO: 1, SEC ID NO: 2, SEC ID NO: 7, and / or SEC ID NO: 8.A sufficient DNA molecule is provided for use as a DNA probe that is useful for determining, detecting, or diagnosing the presence and / or absence of the MON 87708 soybean event DNA in a sample such as SEC ID NO: 11. Other probes may be. IF-2019-80266805-APN-ANP#INPI Page 33 of 50 Easily designed by a person skilled in the art, these kits should comprise at least 15 contiguous nucleotides of SEC ID NO: 6 and be sufficiently unique to the MON 87708 soybean event DNA to identify DNA derived from the event. Another type of kit comprises a primer pair useful for producing an amplicon for detecting the presence and / or absence of MON 87708 transgenic soybean event-derived DNA in a sample. This type of kit employs a method comprising contacting a target DNA sample with a primer pair as described in this invention, then carrying out a nucleic acid amplification reaction sufficient to produce an amplicon comprising SEC ID NO: 1, SEC ID NO: 2, SEC ID NO: 7, and / or SEC ID NO: 8, and then detecting the presence and / or absence of the amplicon.This type of method may also include sequencing the amplicon or a fragment thereof, which would be diagnostic for the presence of the specific DNA for the MON 87708 soybean event in the target DNA sample. Other primer pairs can be easily designed by a skilled practitioner and should comprise at least 15 contiguous nucleotides of SEC ID NO: 6 and should be sufficiently unique to the MON 87708 soybean event DNA in order to identify DNA derived from the event. Nucleic acid amplification can be achieved by any of the various nucleic acid amplification methods known to the art, including thermal amplification methods. Many techniques are known for detecting, quantifying, and / or sequencing the amplicon produced by these methods. An exemplary technique useful for implementing this invention is TAQMAN® (PE Applied Biosystems, Foster City, CA). The detection kits and methods of the invention are useful for, among other things, identifying the MON 87708 soybean event, selecting plant varieties or hybrids comprising the MON 87708 soybean event, and detecting the presence IF-2019-80266805-APN-ANP#INPI Page 34 of 50 of DNA derived from the transgenic soybean event MON 87708 in a sample, and monitor the samples to determine the presence and / or absence of the soybean event MON 87708 or plant parts derived from the soybean event MON 87708. The sequence of the heterologous DNA insert, joining sequences, or flanking sequences of the MON 87708 soybean event (with representative seed samples deposited as ATCC PTA-9670) can be verified (and corrected if necessary) by amplifying such event sequences using primers derived from the sequences provided in this invention followed by conventional DNA sequencing of the amplicon or cloned DNA. As used in this invention, the term “comprising” means “including but not limited to”. The following examples are included to demonstrate examples of certain preferred embodiments of the invention. Those skilled in the art should appreciate that the techniques described in the examples below represent approaches that the inventors have found to work well in the practice of the invention and, therefore, may be considered as examples of preferred embodiments. However, those skilled in the art should appreciate, in light of the present description, that many changes can be made to the specific embodiments described and still obtain a similar or comparable result without departing from the spirit and scope of the invention. EXAMPLES Example 1: Transformation of Soybean A3525 and selection of event MON 87708 The MON 87708 soybean plant was produced by Agrobacterium soybean-mediated transformation. Soybean cells were transformed and IF-2019-80266805-APN-ANP#INPI Page 35 of 50 Regenerated in intact soybean plants, individual plants were selected from the plant population that showed integrity of the plant expression cassette and resistance to dicamba. From this population, the MON 87708 soybean plant event was selected and characterized. The transgenic dicamba-tolerant soybean plant MON 87708 was developed through Agrobacterium-mediated transformation of soybean meristem tissue using the transformation vector PV-GMHT4355. The method, described in U.S. Patent No. 6,384,301 (incorporated herein by reference), allows for the generation of transformed plants without the use of callus. Briefly, meristem tissues were cut from germinated A3525 soybean seed embryos (Asgrow, St. Louis, MO). After co-culture with Agrobacterium carrying the vector, the meristems were placed in selection medium containing glyphosate (Monsanto, St. Louis, MO), carbenicillin disodium salt, cefotaxime sodium salt, and a ticarcillin / clavulanate disodium salt mixture to inhibit the growth of non-transformed plant cells and excess Agrobacterium.The meristems were then placed in media that promote shoot and root development. Rooted plants with normal phenotypic characteristics were selected and transferred to soil for growth assessment and further evaluation. The R0 plants generated through the aforementioned transformation were transferred to soil for growth and then self-fertilized to produce the R1 seed. During the subsequent self-fertilization of the R0 plants to produce the R1 generation, the unlinked insertions of T-DNA I (dmó expression cassette) and T-DNA II (cp4 epsps expression cassette) were segregated. A non-lethal dose of glyphosate was applied to the R1 plants. Plants with minor lesions were selected for further analysis, while the plants IF-2019-80266805-APN-ANP#INPI Page 36 of 50 Plants that showed no lesions, i.e., those containing T-DNA II (cp4 epsps expression cassette), were eliminated from further development. Subsequently, R0 plants containing only a single T-DNA insert (i.e., dmo gene cassette) were identified. The T-DNA I expression cassette comprised the Peanut Chlorotic Streak Virus (PCISV) promoter with a duplicated enhancer region (P-PCISV).FLt-enh) operationally linked to a guide DNA derived from Tobacco Etch Virus (L-TEV) RNA transcript; operationally linked to a DNA molecule encoding an N-terminal chloroplast transit peptide of the Pisum sativum ribulose 1,5-bisphosphate carboxylase (SSU) small subunit (TS-RbcS-3C); operationally linked to part of the mature protein of the Pisum sativum ribulose 1,5-bisphosphate carboxylase (SSU) small subunit (CR-RbcS-3C); operationally linked to a DNA molecule encoding a dicamba monooxygenase (DMO) from Stenotrophomonas maltophilia (Pseudomonas maltophilia was the original source name of the DMO gene).This source organism was subsequently reclassified first as Xanthomonas maltophilia and then as Stenotrophomonas maltophilia; operationally linked to a 3' UTR DNA molecule derived from the Pisum sativum ribulose 1,5-bisphosphate carboxylase small subunit gene (TPs.RbcS2-E9). Plants were selected using a combination of analytical techniques, including TaqMan, PCR analysis, and herbicide spraying. Event MON 87708 was selected from approximately 2,400 individual transgenic events based on its superior phenotypic characteristics, a comprehensive molecular profile analysis, and its association with the desired haplotype. Event MON 87708 was then crossed with event MON 89788 (glyphosate-tolerant). The progeny of this cross were treated with dicamba (Clarity®, BASF). IF-2019-80266805-APN-ANP#INPI Page 37 of 50 Research Triangle Park, NC), glyphosate (Roundup WeatherMAX®, Monsanto Co., St. Louis, MO), or a combination of dicamba and glyphosate. Treatments were conducted preplant, postplant at the vegetative growth stage (V3), and postplant at the reproductive stage (R1). The treated plants were graded to determine the percentage of growth inhibition at 14 days post-treatment (DPT) for the preplant herbicide treatment, 3 DPT for the post-emergence treatment at the VE stage, and 3 DPT for the post-emergence treatment at the R1 stage. The herbicide(s) were applied at various rates per acre as shown in Table 10. Percentage inhibition measurements represent an average of replicates. Table 1: Tolerance test to Dicamba and / or Roundup WeatherMAX® with MON89788 x MON 87708 Herbicide (ea Ratio gm / ha (lb / a)) % inhibition at 14 DDT PRE % inhibition at 3 DDT POST (V3) % inhibition at 3 DDT POST (R1) Untreated / No herbicide 0.0 0.0 0.0 Roundup WeatherMAX® (3364 (3.0)) 0.0 0.0 0.0 Clarity® (2244 (2.0)) 0.0 10.0 20.0 Clarity®561 (0.5) and Roundup WeatherMAX® (841 (0.75)) 0.0 5.0 10.0 Clarity® (1120 (1.0)) and Roundup WeatherMAX® (1682 (1.5)) 0.0 7.5 12.5 Clarity® (2244 (2.0)) and Roundup WeatherMAX® (3364 (3,0)) 0,0 22,5 25,0 The dicamba tolerance transgene was mapped in the soybean event MON 87708 up to linkage group 9 at approximately map position 143.5. The associated haplotype window 19743 and 19767 has no effect on yield, maturity, height, or lodging. Information is provided. IF-2019-80266805-APN-ANP#INPI Page 38 of 50 haplotype association information in Table 2 where GM_A92205 indicates event MON 87708. Table 2: Association of haplotype LG9, Pos 143.5Haplotype Window Event Haplotype ID Yield Maturity Height Acame Haplotype Sequence Linkage Group GM_A92205 19743 1573355 0.00 -0.03 0.06 0.04 CGCTG 9 GM_A9 0.00 0.07 -0.03 -0.04 CGCTA 9 GM_A92205 19743 1573371 0.00 -0.09 -0.41 -0.09 CCCTG 9 GM_A92205 19743 1573373 -0.0.00 -0.01 -0. TG*GG 9 GM_A92205 19743 1573374 0.00 -0.08 -0.07 0.05 TG*GA 9 GM_A92205 19743 1573375 0.00 -0.15 0.05 0.04 CCCTA920 GM_A955 19743 1573376 0.00 -0.45 -0.14 0.00 TC*GG 9 GM_A92205 19767 1573486 0.00 0.00 -0.03 0.00 TACGGTC 9 GM_A929207349 0.00 0.00 0.22 0.00 AACAATT 9 GM_A92205 19767 1573494 0.00 0.00 0.03 0.00 TACAATC 9 GM_A92205 19767 1573495 0.000 0.000 TGAAACC 9 GM_A92205 19767 1573497 0.00 0.00 0.41 0.00 TACGGTT 9 GM_A92205 19767 1573499 0.00 0.00 -0.01 0.00 TGAACT_2092 19767 1573500 0.00 0.00 0.06 0.00 TGAGACC 9 GM_A92205 19767 1573502 0.00 0.00 -0.07 0.00 AACAATC 9 GM_A92205 197350 157305 0.00 0.08 0,00 AACGATC 9 GM_A92205 19767 1573504 0.00 0.00 0.07 0.00 TACAGTC 9 GM_A92205 19767 1573506 0.00 0.00 -0.03 0.00 AACGATT 9 GM_A92205 19767 1573507 0.00 0.00 0.20 0.00 TGAAATT 9, Example 2: Characterization of DNA Sequences of MON 87708 The DNA inserted into the MON 87708 soybean plant genome and the flanking sequence were characterized using detailed molecular analyses. These analyses included: the insertion sequence, the number of insertions (number of integration sites within the soybean genome), the copy number (number of copies of the transgenic DNA within a locus), and the IF-2019-80266805-APN-ANP#INPI Page 39 of 50 integrity of the inserted gene cassette, flanking sequences, and association of the insertion with haplotype regions of the soybean genome. Molecular DNA probes were used that included the intact coding region and its respective regulatory elements, promoters, introns, and polyadenylation sequences from the plant expression cassettes. The analysis showed that MON 87708 contains a single transgenic DNA insert with one copy of the expression cassette. DNA sequence and reverse PCR analyses performed to determine the 5' and 3' junctions of the insert genome to the plant confirm the organization of the elements within the insert (Figure 1) and determine the complete DNA sequence of the insert in the MON 87708 soybean plant (provided in this invention as SEC ID NO: 5). A soybean plant comprising in its genome the linked transgenic genetic elements shown in Figure 1 and resistant to dicamba is an aspect of the invention. The sequences flanking the transgenic DNA insertion in MON 87708 were determined using reverse PCR as described in Ochman et al., 1990 (PCR Protocols: A Guide to Methods and Applications, Academic Press, Inc.) and / or Genome Walker techniques. Plant genomic DNA was isolated from A3525 and transgenic soybean lines from tissue grown under conventional greenhouse conditions. Approximately 1 gram of young leaf tissue was combined with liquid nitrogen and ground to a fine powder using a mortar and pestle. DNA was extracted using a Nucleon™ PhytoPure™ Genomic DNA Extraction Kit (RPN8511, Amersham, Piscataway, NJ) according to the manufacturer's protocol. After the final precipitation step, the DNA was resuspended in 0.5 ml of TE (10 mM Tris-HCl, pH 8.0, 1 mM EDTA). This method can be modified by a person skilled in the technique to extract DNA from any soybean tissue, including, but not limited to, IF-2019-80266805-APN-ANP#INPI Page 40 of 50 The seed tissue. An aliquot of DNA was digested with restriction endonucleases selected based on restriction analysis of the transgenic DNA. After self-ligation of restriction fragments, PCR was performed using primers designed from the transgenic DNA sequence that would amplify sequences extending from the 5' and 3' ends of the transgenic DNA. PCR products were separated by agarose gel electrophoresis and purified using a QIAGEN gel purification kit (Qiagen, Valencia, CA). Subsequent DNA products were directly sequenced using conventional DNA sequencing protocols. The 5' flanking sequence extending into the right-edge sequence of the transgenic DNA in the expression cassette is presented as SEC ID NO: 3 ([C], see Figure 1).The flanking 3' sequence extending into the left-edge sequence of the transgenic DNA in the expression cassette is presented as SEC ID NO: 4 ([D], see Figure 1). The portion of the DNA in the expression cassette that was fully integrated into the A3525 genomic DNA is presented as SEC ID NO: 5 ([E], see Figure 1). The sequences of the isolated DNA molecule were compared with the sequence of the transgenic DNA to identify the flanking sequence and the co-isolated transgenic DNA fragment. Confirmation of the presence of the expression cassette was achieved by PCR with primers designed based on the deduced flanking sequence data and the known transgenic DNA sequence. The wild-type sequence corresponding to the same region in which the transgenic DNA was integrated into the transformed line was isolated using primers designed from the flanking sequences in MON 87708. PCR reactions were performed using the Elongase® amplification system (Invitrogen, Carlsbad, CA). The flanking DNA sequences in MON 87708 and the wild-type sequence A3525 were IF-2019-80266805-APN-ANP#INPI Page 41 of 50 The sequences were analyzed against multinucleotide and protein databases. This information was used to examine the relationship of the transgene to the plant genome and to assess the integrity of the insertion site. The flanking and wild-type sequences were used to design primers for TAQMAN® endpoint assays used to identify events. Zygosity assays were developed using this information. Example 3: Event-Specific TAQMAN® Criterion-Specific Trials This example describes an event-specific TAQMAN® thermal amplification method developed to identify event MON 87708 in a sample. Examples of useful conditions with the event-specific TAQMAN® method for event MON 87708 are as follows: Step 1: 18 megahom of water adjusted to a final volume of 10 pl. Step 2: 5.0 µL of 2X Universal Master Mix (dNTPs, enzyme, buffer) to a final concentration of 1X.Step 3: 0.5 µL Event Primer 1 (SQ13570) and Event Primer 2 (SQ13571) Mixture (resuspended in 18 megahoms of water to a concentration of 20 µM for each primer) to a final concentration of 1.0 µM (e.g., in a microcentrifugation tube, the following should be added to achieve 500 µL to a final concentration of 20 µM: 100 µL of Primer SQ13570 (SEC ID NO: 9) at a concentration of 100 µM; 100 µL of Primer SQ13571 (SEC ID NO: 10) at a concentration of 100 µM; 300 µL of 18 megahoms of water). Step 4: 0.2 μL of Event Probe 6-FAM™ MGB PB4655 (resuspended in 18 megahomi of water to a concentration of 10 μM (SEC ID NO: 11) to a final concentration of 0.2 μM. Step 5: 0.5 μL of Internal Control Primer 1 Mixture and Internal Control Primer 2 (resuspended in 18 megahomi of. IF-2019-80266805-APN-ANP#INPI Page 42 of 50 water to a concentration of 20 μM for each primer) to a final concentration of 1.0 μM. Step 6: 0.2 pl of VIC™ Internal Control Probe to a final concentration of 0.2 μM (resuspended in 18 megahom of water to a concentration of 10 μM). Step 7: 3.0 μL of Extracted DNA (template) for each sample, with each comprising: 1. Leaf samples to be tested; 2. Negative control (non-transgenic DNA); 3. Negative water control (no template); 4. Positive control DNA MON 87708. Step 8: The thermocycler conditions are as follows: One cycle at 50°C for 2 minutes; One cycle at 95°C for 10 minutes; Ten cycles of 95°C for 15 seconds then 64°C for 1 minute with -1°C / cycle; Thirty cycles of 95°C for 15 seconds then 54°C for 1 minute; final cycle of 10°C. The DNA primers used in the endpoint assay are primers SQ13570 (SEC ID NO: 9), SQ13571 (SEC ID NO: 10), and the probe labeled 6-FAM™ PB4655 (SEC ID NO: 11). 6-FAM™ is a fluorescent dye product from Applied Biosystems (Foster City, CA) bound to the DNA probe. For TAQMAN® MGB™ probes, the 5' exonuclease activity of Taq DNA polymerase dissociates the probe at the 5' end, between the fluorophore and the template. When hybridized to the target DNA strand, the template and fluorophore are sufficiently separated to produce a fluorescent signal, thereby releasing fluorescence. SQ13570 (SEC ID NO: 9) and SQ13571 (SEC ID NO: 10) when used with these reaction methods with PB4655 (SEC ID NO: 11) produce a DNA amplicon that is diagnostic for MON 87708 event DNA.The controls for this analysis should include a positive control of soybeans containing DNA from the MON 87708 event, a negative control of non-GMO soybeans, and a negative control that does not contain template DNA. Additionally, a control for the PCR reaction includes Internal Control Primers and an Internal Control Probe, specific to one. IF-2019-80266805-APN-ANP#INPI Page 43 of 50 single-copy gene in the Glycine genome. A person skilled in the art will know how to design primers specific to a single-copy gene in the Glycine genome. These assays are optimized for use with either an Applied Biosystems GeneAmp® POR System 9700 (operated at full speed) or an MJ Research DNA Engine PTC-225 thermal cycler. Other methods and apparatus known to those skilled in the art that produce amplicons identifying the DNA of event MON 87708 are within the art's knowledge. R0 plants showing the presence of the expression cassette were allowed to develop into fully mature plants. Probes designed based on the sequences of the dicamba tolerance transgene cassette were used to probe Southern blots to determine linkage. R0 plants were also evaluated to determine the number of expression cassette copies using a combination of Southern blot analysis and TAQMAN® endpoint analysis. A zygosity assay is useful for determining whether a plant comprising an event is homozygous for the event DNA; that is, it comprises the exogenous DNA at the same location on each chromosome of a chromosome pair; or heterozygous for the event DNA, that is, it comprises the exogenous DNA on only one chromosome of a chromosome pair; or null for the event DNA, that is, wild-type. The TAQMAN® thermal amplification method with endpoints was also used to develop zygosity assays for the MON 87708 event. This example describes an event-specific TAQMAN® thermal amplification method developed to determine the zygosity of the MON 87708 event in a sample. For this assay, a three-primer assay was used where primer SQ20632 (SEC ID NO: 12) is hybridized and extended IF-2019-80266805-APN-ANP#INPI Page 44 of 50 Specifically, from the 3' junction of the inserted exogenous DNA and genomic DNA, primer SQ20636 (SEC ID NO: 13) hybridizes and extends specifically from the DNA flanking the 3' side of the inserted exogenous DNA, and primer SQ20637 (SEC ID NO: 14) hybridizes and extends specifically from the genomic DNA into which the inserted exogenous DNA was integrated. All three primers are diagnostic for the event. In this example, primer SQ20636 (SEC ID NO: 13), primer SQ20632 (SEC ID NO: 12), and the 6-FAM™ PB10130 oligonucleotide probe (SEC ID NO: 15) are diagnostic when one copy of the inserted exogenous DNA is present. In this example, SQ20636 (SEC ID NO: 13) and the primer SQ20637 (SEC ID NO: 14) and the VIC™ labeled oligonucleotide probe PB10131 (SEC ID NO: 16) are diagnostic when there is no copy of the inserted exogenous DNA present in the genomic DNA, i.e., wild type.When the three primers and two probes are mixed together in a PCR reaction with DNA extracted from a plant homozygous for the MON 87708 event, a fluorescent signal is present only from the oligonucleotide probe labeled 6-FAM™ PB10130 (SEC ID NO: 15), which is indicative of, and diagnostic of, a plant homozygous for the MON 87708 event. When the three primers and two probes are mixed together in a PCR reaction with DNA extracted from a plant heterozygous for the MON 87708 event, a fluorescent signal is present from both the oligonucleotide probe labeled 6-FAM™ PB10130 (SEC ID NO: 15) and the oligonucleotide probe labeled VIC™ PB10131 (SEC ID NO: 16), which is indicative of, and diagnostic of, a plant heterozygous for the MON 87708 event.When the three primers and the two probes are mixed together in a PCR reaction with DNA extracted from a plant that is null for the MON 87708 event (i.e., wild type), there is a fluorescent signal only from the oligonucleotide probe labeled VIC™ PB10131. IF-2019-80266805-APN-ANP#INPI Page 45 of 50 (SEC ID NO: 16) which is indicative of, and diagnostic of, a null plant for the MON 87708 event, i.e., wild type. Examples of useful conditions with this method are as follows. Step 1: 18 megahomi of water adjusted to a final volume of 10 µL. Step 2: 5.0 µL of Universal Master Mix 2X (Applied Biosystems cat # 4304437; dNTPs, enzyme, buffer) to a final concentration of 1X. Step 3: 0.5 µL of Zygosity Primers SQ20632, SQ20636, SQ20637 (resuspended in 18 megahomi of water to a concentration of 20 µM for each primer) to a final concentration of 1.0 µM. Step 4: 0.2 µL of 6-FAM™ Probe PB10130 (SEC ID NO: 15) (resuspended in 18 megahoms of water to a concentration of 10 µM) to a final concentration of 0.2 µM. Step 5: 0.2 µL of VIC™ Probe PB10131 (SEC ID NO: 16) (resuspended in 18 megahoms of water to a concentration of 10 µM) to a final concentration of 0.2 µM.Step 6: 3.0 µL of extracted DNA (template) for each sample, comprising one of the following: 1. Leaf samples to be tested (4–80 ng of genomic DNA diluted in water); 2. Negative control (non-GMO soybean DNA; 4 ng diluted in water); 3. Negative water control (no template; solution in which the DNA was resuspended); 4. MON 87708 genomic DNA positive control for known heterozygous event (4 ng diluted in water); 5. MON 87708 genomic DNA positive control for known homozygous event (4 ng diluted in water). Step 7: Mix gently. Step 8: The following are the thermocycler conditions when using the Applied Biosystems GeneAmp® 9700 PCR System (operated at maximum speed) or MJ Research DNA Engine PTC-225 thermal cycler.One cycle at 50°C for 2 minutes; one cycle at 95°C for 10 minutes; Ten cycles of (95°C for 15 seconds, then 64°C for 1 minute (-1°C / cycle); Thirty cycles of (95°C for 15 seconds, then 54°C for 1 minute); 10 to 20 additional optional cycles (95°C for 15 seconds, then 64°C for 1 minute. IF-2019-80266805-APN-ANP#INPI Page 46 of 50 (-1 C / cycle) can provide a more distinctive population separation during TaqMan® EndPoint Assessment Criterion analysis; One cycle at 10°C maintain. Example 4: Identifying the MON 87708 event in any MON 87708 genetic improvement activity The following example describes how the MON 87708 event can be identified within the progeny of any genetic improvement activity using the MON 87708 soybean event. DNA event primer pairs are used to produce a diagnostic amplicon for the MON 87708 soybean event. A diagnostic amplicon for MON 87708 comprises at least one binding sequence, provided as SEC ID NO: 1 or SEC ID NO: 2 or SEC ID NO: 7 or SEC ID NO: 8. The event primer pairs that will produce a diagnostic amplicon for MON 87708 include primer pairs based on the flanking sequences and the inserted expression cassette. To acquire a diagnostic amplicon in which SEC ID NO: 1 is found, one would design a forward primer molecule based on SEC ID NO: 3 from bases 1 to 1126 and a reverse primer molecule based on the DNA sequence of the inserted expression cassette (SEC ID NO: 5 from positions 1 to 3003) where the primer molecules are of sufficient contiguous nucleotide length to hybridize specifically to SEC ID NO: 3 and SEC ID NO: 5.To acquire a diagnostic amplicon in which SEC ID NO: 2 is found, one would design a forward primer molecule based on the DNA sequence of the inserted expression cassette (SEC ID NO: 5 from positions 1 to 3003) and a reverse primer molecule based on the flanking sequence at 3' (SEC ID NO: 4 from bases 131 to 1947), wherein the primer molecules are of sufficient length of contiguous nucleotides to hybridize specifically to. IF-2019-80266805-APN-ANP#INPI Page 47 of 50 SEC ID NO: 4 and SEC ID NO: 5. For practical purposes, one should design primers that produce amplicons of a limited size range, for example, between 100 and 1000 bases. Smaller amplicons (shorter polynucleotide lengths) are generally produced more reliably in PCR reactions, allow for shorter cycle times, and can be easily separated and visualized on agarose gels or adapted for use in TAQMAN®-type endpoint assays. Smaller amplicons can be produced and detected using methods known in DNA amplicon detection techniques. In addition, the amplicons produced using the primer pairs can be cloned into vectors, propagated, isolated, and sequenced, or they can be directly sequenced using well-established methods in the technique.Any primer pair derived from the combination of SEC ID NO: 3 and SEC ID NO: 5 or the combination of SEC ID NO: 4 and SEC ID NO: 5 that is useful in a DNA amplification method for producing a diagnostic amplicon for MON 87708 or its progeny is an aspect of the invention. Any single isolated DNA polynucleotide primer molecule comprising at least 11 contiguous nucleotides of SEC ID NO: 3, or its complement, that is useful in a DNA amplification method for producing a diagnostic amplicon for MON 87708 or its progeny is an aspect of the invention. Any single isolated DNA polynucleotide primer molecule comprising at least 11 contiguous nucleotides of SEC ID NO: 4, or its complement, that is useful in a DNA amplification method for producing a diagnostic amplicon for MON 87708 or its progeny is an aspect of the invention.Any single isolated DNA polynucleotide primer molecule comprising at least 11 contiguous nucleotides of SEC ID NO: 5, or its complement that is useful in a DNA amplification method to produce a. IF-2019-80266805-APN-ANP#INPI Page 48 of 50 A diagnostic amplicon for MON 87708 or its progeny is an aspect of the invention. An example of the amplification conditions for this analysis is illustrated in Example 3. However, any modification of these methods or the use of DNA primers homologous or complementary to SEC ID NO: 3 or SEC ID NO: 4 or DNA sequences of the genetic elements contained in the transgene insert (SEC ID NO: 5) of MON 87708 that produce a diagnostic amplicon for MON 87708 is within the technique. A diagnostic amplicon comprises a DNA molecule homologous or complementary to at least one genomic / transgenic junction DNA (SEC ID NO: 1 or SEC ID NO: 2 or SEC ID NO: 7 or SEC ID NO: 8), or a substantial portion thereof. An analysis for the plant tissue sample of event MON 87708 should include a positive tissue control of event MON 87708, a negative control of a soybean plant that is not event MON 87708 (e.g., but not limited to A3525), and a negative control that does not contain soybean genomic DNA. A pair of primers that will amplify an endogenous soybean DNA molecule will serve as an internal control for the DNA amplification conditions. Additional primer sequences may be selected from SEC ID NO: 3, SEC ID NO: 4, or SEC ID NO: 5 by those skilled in the art of DNA amplification methods, and the conditions selected for the production of an amplicon using the methods shown in Example 3 may differ, but they result in a diagnostic amplicon for event MON 87708 DNA. The use of these DNA primer sequences with modifications to the methods of Example 3 is within the scope of the invention.The amplicon produced by at least one DNA primer sequence derived from SEC ID NO: 3, SEC ID NO: 4, or SEC ID NO: 5 that is diagnostic for MON 87708 is an aspect of the invention. IF-2019-80266805-APN-ANP#INPI Page 49 of 50 DNA detection kits containing at least one DNA primer of sufficient length of contiguous nucleotides derived from SEC ID NO: 3, SEC ID NO: 4, or SEC ID NO: 5, which, when employed in a DNA amplification method, produces a diagnostic amplicon for MON 87708 or its progeny, is an aspect of the invention. A MON 87708 soybean plant, plant part, plant cell, seed, or commodity that will produce a diagnostic amplicon for MON 87708 when assayed in a DNA amplification method is an aspect of the invention. The assay for the MON 87708 amplicon can be performed using an Applied Biosystems GeneAmp® 9700 PCR System (operated at maximum speed) or an MJ Research DNA Engine PTC-225 thermal cycler or any other amplification system that can be used to produce a diagnostic MON 87708 amplicon as shown in Example 3. A deposit of a representative sample of MON 87708 soybean seed, as described above and mentioned in the claims, has been made pursuant to the Budapest Treaty with the American Standard Crop Collection (ATCC), 10801 University Boulevard, Manassas, VA 20110. The ATCC accession number for this deposit is PTA-9670. The deposit will be maintained with the depository for a period of 30 years, or 5 years after the last filing, or for the effective life of the patent, whichever is longer, and will be replaced as necessary during that period. Having illustrated and described the principles of the invention, it should be evident to those skilled in the art that the invention can be modified in arrangement and detail without departing from those principles. We claim all modifications that are within the spirit and scope of the appended claims. IF-2019-80266805-APN-ANP#INPI Page 50 of 50 Argentine Republic - National Executive Branch 2019 - Year of Exports Additional Signature Sheet Graphic Report Number: IF-2019-80266805-APN-ANP#INPI CITY OF BUENOS AIRES Thursday, September 5, 2019 Reference: 20190101909 The document was imported by the GEDO system with a total of 50 page(s). Digitally signed by GESTION DOCUMENTAL ELECTRONICA - GDE DN: cn=ELECTRONIC DOCUMENTARY MANAGEMENT - GDE, c=AR, o=GOVERNMENT SECRETARY OF MODERNIZATION, ou=ADMINISTRATIVE MODERNIZATION SECRETARY, serialNumber=CUIT 30715117564 Date: 2019.09.05 04:38:21 -03'00' Darío Julio Martin Mayares Administrative Advisor National Patent Administration National Institute of Industrial Property Digitally signed by GESTION DOCUMENTAL ELECTRONICA GDE DN: cn=GEST10N DOCUMENTAL ELECTRONICA - GDE, c=AR, o=SECRETARIA DE GOBIERNO DE MODERNIZACION, ou=SECRETARIA DE MODERNIZACION ADMINISTRATIVA, serialNumber=CUIT 30715117564 Date: 2019.09.05 04:38:23 -03'00'

Claims

1. A diagnostic composition characterized in that it comprises a first DNA primer and a second DNA primer, wherein said first DNA primer comprises at least 15 contiguous nucleotides between nucleotides 1127-4129 of SEQ ID NO: 6, or a complement thereof, wherein said second primer comprises at least 15 contiguous nucleotides between nucleotides 1-1126 or between nucleotides 4130-5946 of SEQ ID NO: 6, or its complement thereof, and wherein the first DNA primer and the second DNA primer produce an amplicon selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2 and their complements when employed together in an amplification reaction with DNA derived from event MON 87708, wherein event MON 87708 comprises SEQ ID NO:

6. Three claims follow.