METHOD FOR DIFFERENTIATING PLURIPOTENT STEM CELLS INTO CARDIOMYOCYTES

Claims

The present invention describes a method for improving the production of cardiomyocytes from induced pluripotent stem cells (iPSCs) by adjusting the culture medium change process during the transition from WNT activation to differentiation inhibition. Instead of completely replacing the culture medium, this innovative technique involves a partial medium change, allowing controlled exposure to WNT activators concurrent with the introduction of WNT inhibitors. This key modification to the protocol markedly increases the consistency and efficiency of cardiomyocyte generation, reducing batch-to-batch variability. By adjusting the medium change process, this method optimizes the creation of cardiomyocytes for use in regenerative medicine, emphasizing the critical adjustment phase within the culture conditions.