ADIPOCYTE MATURATION
Patent Information
- Application Number
- ARP20250101106
- Authority / Receiving Office
- AR · AR
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2024-04-25
- Filing Date
- 2025-04-25
- Publication Date
- 2026-10-28
Claims
The present invention relates to a pluripotent stem cell comprising an expression construct for the expression of a Myo1B protein. The invention further provides methods for producing adipocytes comprising pluripotent stem cells and for food comprising adipocytes or pluripotent stem cells.
1. A pluripotent stem cell comprising: i) an expression construct for the expression of a transcription regulatory protein that inserts into a first genetic safe harbor site; and, ii) an expression construct for the expression of a Myo1B protein, wherein the coding sequence for the Myo1B protein is operatively linked to an inducible promoter; wherein the expression construct of ii) inserts into at least one other genetic safe harbor site other than the first genetic safe harbor site, and wherein the inducible promoter is regulated by the transcription regulatory protein.
16. A method for the production of adipocytes, preferably white adipocytes, comprising a) cultivating the pluripotent stem cell according to any of the preceding claims in a proliferation medium, followed by b) inducing adipocyte differentiation by adding the exogenous substance according to claim 8, preferably the proliferation and / or differentiation medium does not comprise insulin and / or dexamethasone.
20. A food product comprising the pluripotent stem cell according to any of claims 1 to 14 or adipocytes obtained by the method according to any of claims 15 to 17.