System for assessing cell viability, cell membrane piercing and selecting a cell for microinjection
Large bore pipettes are used to measure cell resistance and capacitance, addressing the unreliability of existing methods, enabling accurate assessment of cell viability and membrane integrity through resistance threshold comparison, ensuring reliable cell health determination and successful injection.
AU2022330401B2Pending Publication Date: 2026-07-09AGM MEDICAL
Patent Information
- Application Number
- AU2022330401
- Authority / Receiving Office
- AU · AU
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2021-08-18
- Filing Date
- 2022-08-18
- Publication Date
- 2026-07-09
- Estimated Expiration
- 2042-08-18
Smart Images
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Abstract
Detection apparatus for testing biological cells, including a measuring means for measuring an electrical property of a single biological cell present in a solution, sampling means for sampling the solution, a membrane of the cell, and / or an intracellular fluid of the cell, and an electrically insulating component located between the sampling means and the measuring means, containing therein an electrically conductive material, where the electrically insulating component has resistance to an alkaline solution of pH 8 to 11, where the resistance to the alkaline solution includes resistance to formation of microcracks for at least 1 minute upon exposure to the alkaline solution, resistance to electrical insulation failure for at least 12 minutes upon exposure to the alkaline solution, and resistance to visible structural integrity failure for at least 20 minutes upon exposure to the alkaline solution.
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Citation Information
Patent Citations
Methods of assessing cell viability and cell membrane piercing
WO2020077244A1