Engineered guide RNA for increasing efficiency of CRISPR / Cas12f1 system, and use of same

Abstract

In the present disclosure, there is provided an engineered Cas12f1 guide RNA for increasing intracellular gene editing activity of a CRISPR / Cas12f1 system by overcoming limitations of the prior art. The engineered Cas12f1 guide RNA is an engineered form of a naturally occurring guide RNA whose structure is partially modified. In addition, the engineered Cas12f1 guide RNA is characterized in that at least a portion of the scaffold region, which serves to interact with the Cas12f1 protein, is modified. The engineered scaffold region is characterized in that it is different from a scaffold region of a naturally occurring guide RNA. 20 26 21 06 87 28 J ul 2 02 6 2 0 2 6 2 1 0 6 8 7 2 8 J u l 2 0 2 6

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