Method of preparation and applications of spermidine from wheat germ intended for anti-aging, hair loss prevention and weight control

Abstract

The present invention relates to the field of organic synthesis and specifically concerns a method for preparing and using spermidine from wheat germ for anti-aging, hair loss prevention, and weight management. The process comprises the following steps: preparation of raw materials and pretreatment; separation, using a solution, of a crude extract containing spermidine from wheat germ; preliminary purification of the crude extract; fine purification of the previously purified extract; and drying and storage of the finely purified extract.The beneficial effects are as follows: the proposed method and applications allow spermidine of wheat germ origin to fight against aging by promoting cell repair and slowing down cell aging; its anti-inflammatory and nourishing properties can help improve scalp health and reduce hair loss; although spermidine does not directly participate in lipid metabolism, its role in improving cell health and metabolism can indirectly promote weight control.

Description

1 Method for the preparation and applications of wheat germ-derived permidine for anti-aging, hair loss prevention, and weight control. Technical field: The present invention falls within the field of organic synthesis and relates specifically to a method for the preparation and applications of wheat germ-derived permidine for anti-aging, hair loss prevention, and weight control. Background technology: Permidine is a low molecular weight aliphatic compound containing three amine groups; it is one of the natural polyamines present in all living organisms. As an important raw material for pharmaceutical synthesis, it is widely used in the preparation of medicinal intermediates. In the previous technique, spermidine participates in numerous biological processes in the body, such as the regulation of cell proliferation, cell aging, organ development, immunity, as well as various physiological and pathological processes related to cancer.Recent research shows that it also plays an essential role in the synaptic plasticity of the nervous system, oxidative stress, and autophagy. Spermidine also exerts beneficial effects on cardiovascular health, immune function, intestinal health, and brain function. However, its use in the fields of anti-aging, hair loss prevention, and weight control remains poorly documented. Consequently, it is necessary to have a method for preparing and applying spermidine from wheat germ for anti-aging, hair loss prevention, and weight control, to address the lack of experience in these areas in the current state of the art. Spermidine prepared in this way will be able to play a positive role in these applications.25 Content of the invention The objective of the present invention is to provide a method of preparation and applications of permidine of wheat germ intended for anti-aging, hair loss prevention and weight control, to find a solution to the problem of the lack of experience in the prior art concerning the use of permidine in these fields, and to allow the permidine thus obtained to play a positive role in these applications.To achieve this objective, the present invention proposes the following technical solution: a method for the preparation and application of permidine from wheat germ for anti-aging, hair loss prevention, and weight control, said method comprising the following steps: 5. Preparation of raw materials and pretreatment; Separation of a crude extract containing permidine from wheat germ using a suitable solution; Preliminary purification of the crude extract containing permidine; Fine purification of the extract having undergone preliminary purification; 10. Drying of the finely purified extract, then storage. Preferably, the specific operations for pretreatment of the raw materials include: Selecting fresh, high-quality wheat germ as the raw material; Wash the wheat germ to remove impurities and undesirable parts; 15 Proceed to dry and grind the wheat germ to facilitate the subsequent extraction process.Preferably, the specific operations for separating the crude extract containing spermidine from the wheat germ include: Selecting water as the extraction solvent; 20 Performing a solid-liquid extraction or a solvent extraction under controlled conditions of temperature, vacuum, and time, in order to isolate the crude extract containing spermidine from the wheat germ; Separating the extracted liquid from the solid residues by means of methods such as filtration, centrifugation, or other suitable processes. 25 Preferably, the specific operations for preliminary purification of the crude extract containing spermidine include: Subjecting the extracted liquid to preliminary treatment such as concentration or evaporation to reduce its solvent content; Use adsorption, precipitation or chromatography methods to remove part of the impurities and increase the purity of the permidine.Preferably, the specific operations for finely purifying the extract that has undergone preliminary purification include: 5. Using silica column chromatography or crystallization methods to further improve the purity of the permidine; 6. Carefully controlling various parameters, such as temperature, pH, and flow rate, to optimize purification efficiency. Preferably, the specific operations for drying and then storing the finely purified extract include: 10. Drying the purified permidine to remove residual water and remaining solvents; 11. Storing the permidine under appropriate conditions to maintain its stability and activity. Preferably, wheat germ-derived permidine is used in the anti-aging field. Preferably, the permidine of wheat germ origin is applied in the field of hair loss prevention. Preferably, thepermidine of wheatgerm origin is applied in the field of weight control.Preferably, wheat germ-derived laspermidine is applied simultaneously in the fields of anti-aging, hair loss prevention, and weight control.20 Compared to the prior art, the present invention has the following beneficial effects: The preparation method and applications of wheat germ-derived laspermidine proposed in the present invention allow laspermidine to combat aging by promoting cell repair and slowing down cell aging;25 Its anti-inflammatory and nourishing properties can contribute to improving scalp health and reducing hair loss;Although laspermidine does not participate directly in lipid metabolism, its beneficial action on cell health and metabolism can indirectly promote weight control.The present invention also makes it possible to resolve the lack of prior art experience concerning the use of spermidine in the fields of anti-aging, hair loss prevention and weight control, and allows the spermidine obtained to play a positive role in these applications. Specific Embodiments In order to clearly and completely describe the objective and technical solution of the present invention, and to make its advantages more evident, it should be understood that the five specific embodiments described herein constitute only a part of the embodiments of the invention, and not the entirety of them. They serve only to illustrate the embodiments of the invention and should not be considered limiting. All other embodiments that a person skilled in the art can obtain without inventive effort fall within the scope of protection of the present invention.10 Example of implementation1 The present invention proposes a technical solution: a method for preparing and applying wheat germ-derived serum for anti-aging, hair loss prevention, and weight control. Said method comprises the following steps: I. Preparation of raw materials and pretreatment; the operations include: 15 1. Selection of fresh, high-quality wheat germ as raw materials: Observation of appearance: good-quality wheat germ should have a pale yellow or natural white color, be dry on the surface, and free from mold or traces of insects. Germs that are too dark in color or have a damp surface should be avoided, as this may indicate a lack of freshness or poor storage conditions. 20 Odor assessment: Fresh wheat germ gives off a light, natural wheat aroma. If you detect a musty, abnormal, or pungent odor, do not use it. Origin and brand verification: Choose wheat germ from regions with clean environments and good quality water.Choose reputable and certified brands to guarantee the safety and quality of raw materials. 25 2. Washing the wheat germ to remove impurities and undesirable parts: Preparation of washing equipment: use a large container, such as a stainless steel basin or a plastic tub, as well as a sufficient quantity of clean water. Washing process: pour the wheat germ into the container, add water and stir gently to ensure optimal contact between the water and the germ, thus removing dust, particles and other surface impurities. 30 2025 / 5750 BE2025 / 5750 5 Drainage: Using a fine sieve or colander, collect the germ and allow the excess water to drain. This operation can be repeated until the wash water runs clear. 3. Drying and grinding the wheat germ: Drying: 5. Natural drying: Spread the washed germ on gauze or clean paper in a well-ventilated and sunny place. This method, although slower, helps preserve the natural flavor and nutrients of the germ.Low-temperature drying: Use a food dehydrator or an oven set to a low temperature (generally below 50°C). This method is faster, but avoid excessively high temperatures which could burn the germ or alter its nutrients. Grinding: Using a grinder: Place the dried wheat germ in a grinder and adjust the machine to obtain the desired fineness. The ground germ facilitates subsequent extraction steps. Manual grinding: If a grinder is unavailable, a rolling pin or a mortar and pestle can be used to reduce the germ to a powder. This method is slower and less efficient. Thanks to these steps, clean, dry, and ground wheat germ is obtained, ready for the extraction of permidine. During the operations, it is important to maintain a high level of hygiene to avoid any contamination. The washing, drying, and grinding conditions can be adjusted according to actual needs to guarantee a high-quality raw material. 20 II. Separation of a crude extract containing permidine from wheat germ; operational steps: 1.Choosing water as the extraction solvent: Prepare the water. Prepare the extraction container: choose a container suitable for solid-liquid extraction, such as a round-bottom flask or an extraction reactor, and ensure that it is clean and free from impurities. 2. Carry out solid-liquid extraction or solvent extraction under controlled conditions of temperature, pressure, and duration: Temperature control: After mixing the wheat germ with water, place the extraction vessel in a thermostatic water bath or heating device, maintaining the temperature within an appropriate range. The choice of temperature should take into account the solubility and elasticity of the permidine in water, generally between ambient temperature and 60°C. Pressure control: If a pressure extraction method is used, such as high-pressure liquid extraction, adjust the pressure accordingly.The pressure must be chosen according to the boiling point of the water and the characteristics of the wheat germ to maintain the water in a liquid state during extraction. Time control: define an appropriate extraction time according to experimental or procedural requirements. The duration influences the efficiency and purity of the 10-spermidine extraction. In general, it can vary from a few hours to several tens of hours. Agitation and mixing: during extraction, periodically agitate or mix the wheat germ / water mixture to promote dissolution and complete extraction of spermidine. 3. Separate the extracted liquid from the solid residue by filtration, centrifugation or other methods: 15 Filtration: Use a suitable filtration device (filter paper, filter cloth or filter membrane) to roughly separate the extracted liquid from the solid residue. Pour the liquid containing the serum into the filter, let the liquid flow through and retain the residue on the filter.Centrifugation: To remove fine particles and suspended matter, perform centrifugation. Pour the extracted liquid into centrifuge tubes, place them in the centrifuge, and then centrifuge at an appropriate speed and duration. After centrifugation, the liquid containing the serum is found in the upper part of the tube, while the residues and impurities settle at the bottom. Collection of the extracted liquid: Collect the extracted liquid obtained by filtration or centrifugation for subsequent purification steps. It should be noted that these steps constitute a basic extraction process, and that the actual conditions can be adjusted or optimized according to specific needs. It is also essential to ensure safety and hygiene in order to avoid any source of contamination. 30 2025 / 5750 BE2025 / 5750 7 After obtaining the liquid containing the seramidine, further purification is necessary to increase its purity.This purification generally includes the removal of solvents, impurities, and other undesirable components by means of processes such as distillation, crystallization, or chromatographic separation, according to actual needs. III. Preliminary purification of the crude extract containing permidine; specific operations: 1. Preliminary treatment: Concentration: Concentration by evaporation: Place the extract in an open container and heat using a suitable heat source. As the temperature increases, the solvents evaporate gradually, thus allowing the concentration of the extract. It is necessary to control the temperature to prevent the permidine from decomposing or denaturing under the effect of excessive heat. Concentration by freezing: place the extract in a low-temperature environment to cause the crystallization of the solvent and its separation. This method is suitable for 15 heat-sensitive substances, but care must be taken to avoid loss of permidites during the thawing process.Evaporation: Evaporation at atmospheric pressure: For extracts not sensitive to heat, evaporation at atmospheric pressure can be used. The solvents evaporate rapidly under the effect of heating, which allows for obtaining a concentrated extract. Evaporation under reduced pressure: For extracts containing heat-sensitive components, evaporation under reduced pressure is more appropriate. Under vacuum, the evaporation temperature is lowered, which reduces the loss of heat-sensitive substances. 2. Removal of impurities: 25 Adsorption method: Choose a suitable adsorbent, such as activated charcoal or alc. Add the adsorbent to the extract and stir carefully to ensure sufficient contact between the adsorbent and the impurities. Then, separate the adsorbent from the liquid by filtration or centrifugation to remove some of the impurities. 30 2025 / 5750 BE2025 / 5750 8 Precipitation method: Add specific reagents or modify the pH of the extract to cause the formation of a precipitate of impurities.Next, separate the precipitate from the liquid by centrifugation or filtration. Chromatographic method: Separate the extract components using a chromatographic column. The different components have different adsorption and desorption capacities on the column, which allows the permidine to be separated from impurities. Common methods include thin-layer chromatography and column chromatography. Please note that these operations must be adjusted and optimized according to the experimental conditions and the characteristics of the extract. It is also essential to ensure safety and hygiene during handling to avoid any contamination. Once the impurities have been removed, additional purification steps can be applied to obtain a permidine of higher purity. IV.Fine purification of the extract having undergone preliminary purification; specific operational steps: 15 1.Silica Column Chromatography: Silica Column Preparation: Select a suitable silica and ensure that the particle size, pore diameter, and specific surface area meet the experimental requirements. Choose a silica column of appropriate dimensions based on the extract volume and the expected purification effect. Introduce the silica into the column and pre-wash it with a suitable solvent to remove any impurities present in the silica. Sample Loading: Slowly add the pre-treated extract to the silica column, ensuring that the liquid is evenly distributed across the silica surface. Control the flow rate to avoid column clogging or ineffective separation. Elution: Use a suitable eluent; the choice of type and concentration depends on the differences in adsorption between permidine and impurities. Gradually increase the polarity of the eluent to allow the progressive desorption of permidine from the column.Collect the elution fractions and monitor the separation of the different components during the process. 5. Fractionation and analysis: According to experimental needs, collect the elution fractions in segments and analyze them. Analyze the purity of permidine in each fraction using thin-layer chromatography or high-performance liquid chromatography. 2. Crystallization: 10. Concentration and crystallization: Concentrate the extract purified by column chromatography to reduce the neuron in solvent. Choose appropriate crystallization conditions, such as temperature, pH, and solvent type, to promote the progressive crystallization of permidine. 15. Collection and drying of crystals: Collect the permidine crystals by filtration or centrifugation. Use an appropriate drying method, such as video drying or natural drying, to remove residual solvents present in the crystals. 3. Control of conditions: 20 Temperature: Temperature plays a crucial role in column chromatography and crystallization.Too high or too low a temperature can affect separation and the rate of crystallization. It is therefore necessary to choose a suitable temperature according to the experimental conditions and the properties of permidine. 25 pH: The pH strongly influences the elasticity and separation of permidine. During column chromatography and crystallization, the pH of the extract and the eluent must be maintained within a range compatible with the elasticity of permidine to avoid degradation or denaturation. 30 2025 / 5750 BE2025 / 5750 10 Flow rate: In column chromatography, the flow rate directly impacts the separation efficiency. A flow rate that is too fast can lead to incomplete separation, while a flow rate that is too slow prolongs the operating time and reduces overall efficiency. It is therefore advisable to choose a flow rate adapted according to the dimensions of the column, the properties of the silica and those of the extract.5 In summary, careful control of parameters during column chromatography and crystallization significantly improves the purity of the permidine. It is also important to respect good experimental practices and safety rules to guarantee the reliability and precision of the results. V. Drying and Storage of the Finely Purified Extract; Specific Operations: 10 1. Drying: Choice of Drying Method: Depending on the properties of the serum and the experimental conditions, choose an appropriate drying method. Common methods include vacuum drying, freeze-drying (freeze drying), and spray drying. Vacuum drying is suitable for heat-sensitive substances because it allows for the efficient removal of water and solvents at low temperatures. Freeze drying is suitable for preserving the structure and activity of the substance. Adjusting Drying Conditions: 20 Adjust parameters such as temperature, pressure, and time according to the chosen method.For vacuum drying, it is necessary to control the temperature and vacuum level to avoid degradation or denaturation of the permidine. For freeze drying, the freezing and sublimation rates must be controlled to ensure uniform drying and maintain product quality. 25 Drying Operation: Place the purified permidine in the drying apparatus and carry out the drying according to the predefined conditions. During the process, regularly check the progress of the drying to ensure that all residual water and solvents have been removed. Verification of drying effectiveness: 30 2025 / 5750 BE2025 / 5750 11 After drying, verify the effectiveness of the process using appropriate methods (e.g., determination of water content, analysis by infrared spectroscopy). Ensure that the water content and residual solvent in the product meet the required standards. 2.Storage: Choice of storage container: Use airtight and chemically corrosion-resistant containers, such as glass bottles or high-density polyethylene bottles. Ensure that the internal walls of the container are clean and free of impurities to avoid any contamination of the product. Control of storage conditions: Aspermidine must be stored in a dark, dry, and cool place. Light, heat, and humidity can cause its degradation or alteration. It is therefore essential to respect these conditions. Strictly control the storage temperature. In general, a low temperature (2 to 8°C) helps maintain the elasticity and activity of aspermidine. Avoid all contact with harmful substances: 15. Ensure that permidine is not stored with toxic or hazardous substances to avoid cross-contamination or chemical reactions. Regular inspection and recording: Regularly inspect stored permidine: appearance, odor, purity.In case of an anomaly, take corrective measures immediately and record the observations. 20 Also record storage data such as duration, temperature and humidity to be able to monitor the quality and elasticity of the product. By following these steps, it is possible to ensure that the purified permidine retains its elasticity and activity during the drying and storage phases, thus guaranteeing a high-quality product for subsequent uses. 25 Example implementation 2 Based on example implementation 1, wheat germ-derived permidine is applied in the field of anti-aging; The specific application is as follows: Antioxidant action: Aspermidine is an antioxidant that helps eliminate free radicals in the body and protect cells against oxidative damage. This antioxidant property helps reduce the impact of free radicals and slow down the aging process.Promoting DNA repair: Laspermidine can stimulate the activity of DNA repair enzymes, thus accelerating DNA repair and reconstruction and reducing the effects of DNA damage on cells. This is essential for maintaining cellular health and stability, and helps delay cellular aging. Improving cellular energy production: Laspermidine can promote energy production in mitochondria, thus increasing cell metabolism and vitality, which helps delay the cellular aging process. Improving skin condition: Laspermidine can stimulate collagen synthesis in the skin, increasing its elasticity and firmness, and reducing the appearance of wrinkles and fine lines. It can also inhibit the formation and deposition of melanin, thus reducing pigment spots and improving overall skin quality. Immune function enhancement: Spermidine can strengthen the immune system, improve the body's resistance, and reduce the risk of disease.By increasing immune cell activity and antibody production, it helps prevent infections and external aggressions. Although permidine has remarkable potential in the anti-aging field, certain precautions remain necessary. Its bioavailability can be influenced by numerous factors such as age, sex, or diet; it is therefore recommended to consult a professional before use. Furthermore, permidine is not a miracle solution and cannot replace other essential anti-aging measures such as a healthy lifestyle, a balanced diet, and appropriate physical activity. In summary, laspermidine derived from wheat germ has multiple applications in the field of anti-aging and can help maintain a state of health and youth. However, its use should be combined with other anti-aging measures, taking into account individual differences and responsible use.Example implementation 3 Based on example implementation 1, wheat germ-derived permidine is applied in the field of hair loss prevention; Detailed presentation: The use of wheat germ-derived permidine in the field of hair loss prevention is attracting increasing attention. As a bioactive substance, permidine has various functions, including the stimulation of cell proliferation, the improvement of cell metabolism, and an antioxidant action. These properties give it particular potential in the fight against hair loss. First, laspermidine can stimulate blood circulation in the scalp, providing more nutrients and oxygen to the hair follicles. Good blood circulation promotes follicle growth and development, strengthening hair implantation and thus reducing the risk of hair loss. Second, laspermidine can stimulate the proliferation and differentiation of follicular cells.The health and regular growth of hair follicle cells are essential for hair growth and maintenance. By activating certain intracellular signaling pathways, laspermidine promotes the division and differentiation of follicle cells, thus increasing hair density and quantity. Furthermore, laspermidine exerts an antioxidant action, helping to eliminate free radicals in the body and reduce oxidative damage to the scalp and follicles. Oxidative stress is indeed a major factor in hair loss. Thanks to its antioxidant effect, laspermidine helps protect the health of the scalp and follicles and reduce hair loss. In practice, laspermidine extracted from wheat germ can be added to hair products such as shampoos or conditioners. Applied directly to the scalp and hair, it can exert its anti-hair loss effects.Spermidine can also be used as a dietary supplement to improve the overall condition of the scalp and hair health. However, it should be noted that the causes of hair loss and the condition of the scalp vary from person to person. Before using spermidine in a hair loss treatment, it is best to consult a doctor or obstetrician. Furthermore, although spermidine has some potential, it cannot treat all forms of hair loss. Its use should be combined with other measures, including a regular exercise routine, a balanced diet, and reducing aggressive hair treatments such as coloring and perms. In conclusion, permidine from wheat germ has great application prospects in the prevention of hair loss.Thanks to its ability to improve scalp blood circulation, stimulate follicular cell proliferation, and exert an antioxidant action, it contributes to improving scalp health and reducing hair loss. However, its use must be adapted to individual characteristics and accompanied by complementary measures to obtain the best results. Example of implementation 410 Based on example of implementation 1, wheat germ-derived permidine is applied in the field of weight control. Detailed presentation: The application of wheat germ-derived permidine in weight control is generating increasing interest. As an important biological compound, permidine plays a significant role in protein synthesis, strengthening the immune system, and improving physical resistance. In the context of weight control, it can also exert beneficial effects. Firstly, spermidine can increase muscle strength, improve endurance and enhance physical capacity.This is particularly important for people wishing to control their weight through physical activity. By strengthening muscles and improving endurance, permidine allows for longer and more intense training sessions, thus promoting higher calorie expenditure and contributing to weight control. Furthermore, permidine can promote fat metabolism. It helps the body break down and burn fat more efficiently, thus reducing its accumulation. By modulating the lipid metabolism process, permidine contributes to weight control and the reduction of body fat. Furthermore, laspermidine can stimulate the body's overall metabolism. Metabolism corresponds to the process of energy expenditure; by increasing metabolic rate, the body consumes calories more quickly and stores less fat. Its action on improving metabolism thus contributes to the goal of weight control.30 2025 / 5750 BE2025 / 5750 15 It should be emphasized, however, that even though Permidine has some potential in weight control, it is not a "miracle cure." To obtain the best results, it is necessary to combine its use with a balanced diet and regular physical activity. Furthermore, since each individual has different needs and health conditions, it is recommended to consult a doctor or nutritionist before using Permidine for weight control purposes, in order to guarantee safety and effectiveness. In conclusion, wheat germ-derived serpidine shows real potential in the field of weight management. By improving muscle strength, endurance, physical capacity, and by stimulating fat and overall metabolism, it can help people achieve their weight management goals. However, its use must take into account individual differences and be combined with other weight management measures to obtain the most effective results.Example of implementation 5 Presentation of the simultaneous application of spermidine derived from wheat germ in the fields of anti-aging, hair loss prevention, and weight control: 15 First, in the field of anti-aging, the antioxidant and anti-inflammatory action of spermidine helps eliminate free radicals and reduce oxidative damage to cells, thus delaying their aging. Furthermore, spermidine can increase cellular autophagy and promote the elimination of waste products, maintaining cells in a "clean" state and further slowing the aging process. Through these mechanisms, 20 spermidine helps preserve the skin's elasticity and firmness, reduce wrinkles and fine lines, and improve the overall appearance of the skin, which then appears younger and healthier. Furthermore, in the area of ​​hair loss prevention, spermidine promotes blood circulation in the scalp, bringing more nutrients and oxygen to the hair follicles, which promotes their growth and health.It can also stimulate the proliferation and differentiation of follicular cells, thus increasing the quantity and density of hair. Thanks to its antioxidant action, it also reduces oxidative stress that affects the scalp and follicles and helps maintain hair health. Thus, the use of hair products containing wheat germ laspermidine can help reduce hair loss and promote healthy hair growth. Finally, in the area of ​​weight management, laspermidine also plays a positive role. It can increase muscle strength, improve endurance, and enhance physical capacity, allowing for more effective exercise and the burning of more calories. It can also promote fat metabolism, helping the body break down and burn fat more efficiently, reducing its accumulation. Furthermore, by stimulating overall metabolism, laspermidine accelerates the body's energy expenditure, thus supporting weight control goals.In summary, the simultaneous application of laspermidine derived from wheat germ in the fields of anti-aging, hair loss prevention, and weight management allows us to take advantage of its multiple properties. Thanks to its antioxidant and anti-inflammatory actions, and its ability to promote cell proliferation and metabolism, laspermidine helps maintain youthful skin, reduce hair loss, and promote weight management. However, since each individual's needs and health status are different, it is recommended to adapt the use of laspermidine to the individual situation and to consult a doctor or nutritionist to ensure safe and effective use. Although embodiments of the present invention have been described and illustrated, it is It is understood that various modifications, improvements, substitutions, or variations may be made by a technician in the field without departing from the spirit and principle of the invention. The scope of protection of the present invention is defined by the attached claims and their equivalents.20 2025 / 5750 BE2025 / 5750.