COMPOSITION FOR BIOSTIMULATION

Abstract

Biostimulant composition comprising at least one bacterium of the genus Bacillus selected from a group consisting of Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus Velezensis, Bacillus cereus, Bacillus pumilus, Bacillus megaterium, Bacillus thuringiensis, and at least one plant co-product in liquid or solid form, the plant co-product comprising a crude protein content of at least 5%, preferably at least 10% relative to the dry weight of the plant co-product (crude protein weight / dry weight of the plant co-product) and wherein said plant co-product in solid form has a median size greater than 250 µm.

Description

BE2025 / 5014 2 Biostimulant compositions are intended to stimulate the biological processes of plants and help them to develop better, resist environmental stresses, and improve plant productivity. Biostimulant compositions can include various components such as microorganisms, vitamins, proteins, plant hormones, etc. 5 In this context, document AU2021102872 describes a solid and liquid composition (agro-nutritive solid and liquid compost) and a process for its production, comprising microorganisms and nutrients to promote plant growth. The agro-nutritive solid and liquid compost includes a biofertilizer such as Bacillus amylolique faciens, Pseudomonas fluorescens, Pseudomonas putida, Bacillus subtilis, 10 Azospirillum brasilense and poultry fecal waste for plant growth. This document describes multi-microbial compositions comprising at least four different microorganisms in the form of a microbial consortium.US20230159405 relates to a biological fertilizer for biofertilization and plant growth stimulation. The biological fertilizer according to the document15 comprises a pure culture of an AO512 strain of Azospirillum oryzae, a pure culture of an AL20 strain of Azospirillum lipoferum, a pure culture of a PD 2 strain of Pantoea dispersa, a pure culture of a TU2 strain of Bacillus subtilis, a pure culture of an S3 strain of Lysinibacillus sphaericus, and a pure culture of an SR17 strain of Paenibacillus polymyxa.20 Although these documents praise the merits of their technology, and the compositions according to these documents may be of some interest for stimulating plant growth and entering into a concept of sustainable crop development, the compositions according to the prior art have many disadvantages.In particular, the biostimulant compositions of the previous article tend to combine a high number of the 25 different microorganisms, possibly with a high number of decofactors and nutrients, in order to increase the synergies and the spectrum of action of the different microorganisms on plant growth. These biostimulant compositions are therefore complex and time-consuming to produce, making them difficult to industrialize (and therefore expensive): they require the preparation of a mixture of numerous bacteria, which are commonly produced / fermented separately.Furthermore, the mixture of a large number of different microorganisms, each with varying resistances to storage conditions and environmental conditions during administration to plants, can render these biostimulant compositions unsustainable, even ineffective, and / or have a reproducible biostimulant effect if the proportion of the different microorganisms in these compositions is not correctly dosed and / or if the storage conditions and / or environmental conditions during administration to the plant are not optimal. Therefore, there is always a need for a composition that is easily industrialized and stable, while also allowing for an effective and reproducible biostimulation treatment with a broad spectrum of action on plant growth. Objectives of the invention The present invention aims to overcome the disadvantages of the prior art, in particular those described above.10 In particular, the present invention proposes to provide a simple and easily industrializable composition for carrying out an effective biostimulant treatment with a broad spectrum of action on the growth of different types of plants. The present invention also proposes to provide a stable composition allowing for a highly reproducible and controlled biostimulant treatment from one production batch to another and / or according to changing environmental conditions. Summary of the invention To achieve these objectives, the present invention provides a composition for the biostimulation of plant growth comprising a bacterial active agent, said bacterial active agent comprising a Bacillus genera bacterium and a nitrogen-fixing bacterium, characterized in that the quantity of said Bacillus genera bacterium and said nitrogen-fixing bacterium in said bacterial active agent is greater than or equal to 75% by weight relative to the total weight of said bacterial active agent in the composition.The inventors have indeed discovered, surprisingly, that a composition for the biostimulation of plant growth with a simplified bacterial active agent, comprising primarily a bacterium of the genus Bacillus and a nitrogen-fixing bacterium, provides excellent biostimulation efficacy while allowing for a significant simplification of the bacterial formulation and the composition formulation. The composition for the biostimulation of plant growth of the invention is therefore very easily industrialized because it only requires the production of two main types of bacteria. Moreover, and particularly surprisingly, with a "simple" bacterial active agent, this composition is particularly effective. to ensure an optimal biostimulant effect on the growth of different types of plants (in a diversity of growing conditions and environmental stresses).Furthermore, as mentioned, this composition is particularly stable, allowing for a highly reproducible and controlled biostimulant treatment from one production batch to another and / or under changing environmental conditions, compared to the case of a complex mixture of numerous different bacteria whose equilibrium is difficult to achieve and significantly more unstable to maintain and / or control. Other embodiments of the composition for the biostimulation of plant growth according to the present invention are indicated in the attached claims. The present invention also relates to a seed of a plant of interest coated by the composition for the biostimulation of the growth of a plant according to the present invention.15 Other embodiments of the seed of a plant of interest according to the present invention are indicated in the attached claims.The present invention also relates to a method for increasing plant growth comprising the step of administering to said plants the composition according to the present invention, or the coated seed according to the present invention. The present invention also relates to the use of a composition for increasing plant growth (particularly under conditions of nitrogen and / or phosphorus deficiency, and / or as at least a partial replacement for nitrogen and / or phosphorus fertilization), said composition comprising a bacterial active agent, said bacterial active agent comprising a Bacillus genera bacterium and a nitrogen-fixing bacterium, characterized in that the quantity of said Bacillus genera bacterium and said nitrogen-fixing bacterium in said bacterial active agent is greater than or equal to 75%. weight relative to the total weight of said bacterial active agent in the composition. Other embodiments of the use of a composition to increase plant growth according to the present invention are indicated in the 30 annexed claims.The present invention also relates to a biostimulant composition comprising at least one bacterium of the genus Bacillus selected from a group consisting of Bacillus subtilis, Bacillus myloliquefaciens, Bacillus velezensis, Bacillus cereus, Bacillus pumilus, Bacillus megaterium, Bacillus thuringiensis, Bacillus licheniformis, and at least one plant co-product in liquid or solid form, said plant co-product comprising a crude protein content of at least 5%, preferably at least 10%, relative to the dry weight of the plant co-product (crude protein weight / dry weight of the plant co-product), and wherein said plant co-product in solid form exhibits a median size greater than 250µm.Indeed, a biostimulant composition comprising at least one Bacillus-like bacterium and at least one plant co-product comprising one or more amino acids provides a significant biostimulant effect on plant growth while being a "simple" composition, easily industrialized, and perfectly integrated into a sustainable development and circular economy process by providing one or more plant co-products as a source of biomolecules. This source of biomolecules stimulates the growth of the Bacillus-like bacterium in the biostimulant composition when this composition is administered to the plant, while also providing nutrients directly to the plant to enhance its growth. Other embodiments of the biostimulant composition according to the present invention are indicated in the attached claims. The present invention also relates to a seed of a plant of interest coated with the biostimulant composition according to the present invention.Other embodiments of the plant seed according to the present invention are indicated in the attached claims. The present invention also relates to a method for increasing plant growth comprising the step of administering to said plants the biostimulant composition according to the present invention, or the plant seed according to the present invention. Other embodiments of the method for increasing plant growth according to the present invention are indicated in the attached claims.The present invention also relates to the use of a biostimulant composition to increase the growth of a plant, said composition comprising at least one bacterium of the genus Bacillus chosen from a group consisting of 30 Bacillus subtilis, Bacillus myloliquefaciens, Bacillus velezensis, Bacillus cereus, Bacillus pumilus, Bacillus megaterium, Bacillus thuringiensis, Bacillus licheniformis, and at least one plant co-product in liquid or solid form, said plant co-product comprising a crude protein content of at least 5%, preferably at least 10%, relative to the dry weight of the plant co-product (crude protein weight / dry weight of the co-product). plant product) and wherein said plant co-product in solid form has a median size greater than 250µm. Other embodiments of the use of a biostimulant composition to increase the growth of a plant according to the present invention are indicated in the attached claims.Detailed description of the invention. Other features, details and advantages of the invention will become apparent from the description given below, by way of non-limiting example and with reference to the examples.