PROCESS FOR THE PURIFICATION OF NEUTRAL HUMAN DELIGHT OLIGOSACCHARIDES (HMO)
A hybrid purification process using ion exchange and nanofiltration/electrodialysis effectively removes contaminants from HMOs, achieving high yields and purity, addressing the inefficiencies of existing methods and enabling large-scale production for food and feed applications.
BR112016016705B1Inactive Publication Date: 2026-07-07CHR HANSEN HMO GMBH
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Patent Information
- Application Number
- BR112016016705
- Authority / Receiving Office
- BR · BR
- Patent Type
- Patents
- Current Assignee / Owner
- Priority Date
- 2014-01-20
- Filing Date
- 2014-12-23
- Publication Date
- 2026-07-07
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
Technical Problem
Existing methods for purifying human milk oligosaccharides (HMOs) are inefficient, costly, and unable to produce high-purity HMOs in large quantities due to contamination from recombinant materials, proteins, and low scalability, making them unsuitable for food and feed applications.
Method used
A process combining cationic and anionic ion exchange treatments with nanofiltration and electrodialysis to purify HMOs, eliminating recombinant material and proteins, and achieving high purity (>80%) without chromatographic separation.
Benefits of technology
The process achieves high-purity HMOs with yields exceeding 70%, suitable for food and feed applications, while being cost-effective and scalable, with products free of recombinant DNA and proteins.
✦ Generated by Eureka AI based on patent content.
Abstract
PROCESS FOR THE PURIFICATION OF NEUTRAL HUMAN MILK OLIGOSACCHARIDES (HMOs), HUMAN MILK OLIGOSACCHARIDE (HMO), AND USE OF AN HMO. This application describes a simple process for the purification of neutral human milk oligosaccharides (HMOs) produced by microbial fermentation. The process uses a combination of a cationic ion exchanger treatment, an anionic ion exchanger treatment, and a nanofiltration and / or electrodialysis step, which allows for the efficient purification of large quantities of high-purity neutral HMOs. Unlike the purification currently used in the fermentative production of neutral HMOs, the presented process allows for the supply of HMOs without the need for chromatographic separation. The HMOs thus purified can be obtained in solid form by spray drying as a crystalline material or as a sterile filtered concentrate.The HMOs supplied are free of proteins and recombinant material from the recombinant microbial strains used and are therefore very well suited for use in food, nutritional feed and animal feed applications (e.g., pet food).
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