Chemical composition for seed treatment
Patent Information
- Application Number
- BR112021023930
- Authority / Receiving Office
- BR · BR
- Patent Type
- Patents
- Current Assignee / Owner
- Publication Date
- 2026-08-11
Abstract
Description
CHEMICAL COMPOSITION FOR SEED TREATMENT Field of the Invention
[0001] The present invention provides a composition comprising phosphorus, molybdenum, vitamin B12 and an amine. It also provides a method for preparing this composition and a method for preparing seeds. It is also related to the use of a composition comprising phosphorus, molybdenum, vitamin B12 and an amine for the treatment of legume seeds. Background of the Invention
[0002] Seed coating in agriculture is well known in prior art and is carried out for a variety of reasons.
[0003] Seed treatment for pest and disease control has been practiced for hundreds of years, beginning with the use of simple inorganic substances such as arsenic, copper sulfate, and sulfur to control fungal diseases in cereal crops. The first evidence of copper sulfate's activity against wheat was reported by Schulthess in 1761. The first broad-spectrum organic fungicide, Thiram (Tisdale and Flenner), was introduced in 1942, and the first organic insecticidal seed treatment, γhexachlorocyclohexane (Slade), in 1945.
[0004] Early seed treatment products were in powder form, but modern formulations tend to be liquid because they are safer to use and have improved application properties. Liquid seed treatments for plant protection are available in many formulation types, such as solutions, emulsions, concentrates, Petition 870210109957, dated 11 / 26 / 2021, page 9 / 61 2 / 26 Suspension and capsule suspensions. Liquid formulations may be based on organic solvents or water-based.
[0005] Coating seeds with plant nutrients or fertilizers is also known in the prior art. Phosphorus is an interesting nutrient to supply to seeds as it promotes root growth, which increases the plant's ability to absorb nutrients from the soil.
[0006] In the case of legumes, such as soybeans (Glycine max), alfalfa (Medicago sativa), clover (Trifolium), peas (Pisum sativum), chickpeas (Cicer arietinum), green beans (Phaseolus vulgaris), lentils (Lens culinaris) and peanuts (Arachis hypogaea), it is common practice to treat seeds with rhizobium inoculants. Leguminous plants are able to form symbiotic relationships with rhizobia bacteria within the root nodules that can fix atmospheric nitrogen, converting it into forms that the plant can then use.
[0007] The application of rhizobium inoculant to legume seeds can enhance this process and improve productivity. Commercially available inoculant compositions are available in peat-based dry preparations, such as those described in CA512197, or as liquid formulations.
[0008] The application of rhizobium inoculant to legume seeds can enhance this process and improve productivity. Commercially available inoculant compositions are available in peat-based dry preparations, as it is well known that the micronutrients molybdenum (Mo) and cobalt (Co) are involved in the fixation process. Petition 870210109957, dated 11 / 26 / 2021, page 10 / 61 3 / 26 nitrogen in leguminous plants and it has become common practice in agriculture to apply seed treatment compositions containing these two micronutrients along with inoculants. Typically, commercially available seed treatments of this type are based on inorganic salts, such as sodium or ammonium molybdate as a source of molybdenum; and inorganic salts such as cobalt(II) sulfate, dinitrate, dichloride, diacetate, carbonate or hydroxide as a source of cobalt. Chelated forms of cobalt, such as cobalt EDTA, cobalt gluconate, cobalt glucoheptonate and cobalt citrate have also been used as a source of cobalt in such preparations. Typical commercially available products of this type are aqueous liquid compositions containing between about 5% and 15% w / w Mo and between 1% and 2% w / w Co. Examples of commercially available products are: Stoller CoMo (Stoller Enterprises Inc.) containing 6% w / w of Mo as sodium molybdate and 1% w / w of Co as cobalt sulfate; Wuxal Extra CoMo 15 (Aglukon Spezialdunger GmbH & Co KG) containing 150 g / l Mo (10.3% w / w) and 15 g / l Co (1.03% w / w); MolyMix (Spray Gro) containing 4.5% w / w of Mo and 1.8% w / w of Coh as described in CA512197, or as liquid formulations.
[0009] However, all these sources of cobalt pose serious risks to human health. For example, cobalt(II) sulfate, dichloride, dinitrate, diacetate, and carbonate are all classified in the Globally Harmonized System as carcinogenic and toxic to reproduction. Furthermore, under the European Union's REACH regulation, Petition 870210109957, dated 11 / 26 / 2021, page 11 / 61 4 / 26 All these cobalt salts are included in the ECHA List of Substances of Very High Concern for Authorisation. Current practice presents a significant health risk to operators and farmers who handle and apply these products to seeds. In particular, there is a significant risk of exposure to hazardous dust created during the handling of treated seeds. A safer alternative is therefore needed.
[0010] U.S. Patent US5797976A (Yamashita, 1998) discloses compositions comprising a carbon / skeletal energy component, a macronutrient component containing at least one nitrogen source and one phosphorus source, and a vitamin / cofactor component. These compositions can be used in agriculture in various ways, including as a seed coating. In particular, a composition comprising 2% by weight of phosphorus, 0.003% by weight of molybdenum, and 0.02% by weight of cyanocobalamin is disclosed.
[0011] U.S. Patent Application US2004 / 063582A1 (Johnson, 2004) discloses compositions comprising at least one macronutrient, at least one micronutrient, a pest inhibitor, at least one growth regulator, a vitamin / cofactor component, an amino acid component, a penetrant, and an energy source, for the disclosed seed treatment.
[0012] International patent (PCT) WO2008 / 090290A2 (INRA, 2008) discloses synthetic cell culture media compositions comprising small amounts of Petition 870210109957, dated 11 / 26 / 2021, page 12 / 61 5 / 26 phosphorus, molybdenum, ethanolamine and vitamin B12 among other components.
[0013] The English patent GB2568945A (Yara UK, 2019) discloses compositions comprising ethanolamine and phosphorus. Summary of the Invention
[0014] According to a first aspect, a liquid composition is provided comprising phosphorus, molybdenum, cobalt, a solvent and monoethanolamine. The cobalt is present as vitamin B12 and the composition has a pH between 6.0 and 7.5.
[0015] According to another aspect, a method is provided for preparing a liquid composition comprising phosphorus, molybdenum, cobalt, and monoethanolamine. The method comprises the steps of providing a source of phosphorus, a source of molybdenum, a source of vitamin B12, and monoethanolamine; mixing the phosphorus source, the molybdenum source, and the vitamin B12 source with a solvent; and adding an amount of monoethanolamine so that the pH is between 6.0 and 7.5.
[0016] According to another aspect, a method is provided for preparing seeds of legume crops. The method comprises the steps of coating the seeds with the liquid composition described herein, coating the seeds with a composition comprising a rhizobium inoculant, and drying the seeds coated with the two compositions.
[0017] According to another aspect, the use of a liquid composition as defined in this document is provided for the treatment of legume crop seeds, particularly to improve germination. DETAILED DESCRIPTION OF THE INVENTION Petition 870210109957, dated 11 / 26 / 2021, p. 13 / 61 6 / 26
[0018] Unless otherwise defined, all terms used in the disclosure of the invention, including technical and scientific terms, have the meaning as commonly understood by one skilled in the art to which this invention pertains. By way of further guidance, definitions of terms are included for a better appreciation of the teaching of the present invention.
[0019] All references cited in this description are considered incorporated in their entirety by reference.
[0020] As used in this document, the following terms have the following meanings:
[0021] A, uma and o, um, as used in this document, refer to referents in both the singular and plural, unless the context clearly dictates otherwise. As an example, um companheiro refers to one or more of a compartment.
[0022] About, as used in this document, referring to a measurable value, such as a parameter, a quantity, a time duration and the like, is intended to cover variations of + / - 20% or less, preferably + / - 10% or less, more preferably + / - 5% or less, even more preferably + / - 1% or less, and even more preferably + / - 0.1% or less from the specified value, to the extent that such variations are appropriate to realize in the disclosed invention. However, it should be understood that the value to which the modifier about refers is also specifically disclosed. Petition 870210109957, dated 11 / 26 / 2021, p. 14 / 61 7 / 26
[0023] Comprehend, comprehending, and comprehends and “comprehended of,” as used in this document, are synonyms of include, including, includes, or contains, containing, contains, and are inclusive or open terms that specify the presence of what follows, for example, component, and do not exclude or preclude the presence of additional unmentioned components, features, elements, members, steps, known in the art, or disclosed therein.
[0024] The recitation of numerical intervals by periods includes all numbers and fractions included in that interval, as well as the recited periods.
[0025] The expression percentage by weight or % by weight, here and throughout the description, unless otherwise defined, refers to the relative weight of the respective component based on the total weight of the formulation.
[0026] As used herein, a phosphorus source refers to a molecule, salt, or complex containing phosphorus atoms that is available to plants, i.e., plants will absorb the molecule, salt, or complex and will be able to use the phosphorus contained therein for their growth and development. Note that the different vitamins of vitamin B12 contain at least one P atom; however, this P atom is embedded in the vitamin B12 molecule and will not be used by the plant as a P source. Suitable P sources are well known in the field of agriculture and include orthophosphoric acid, polyphosphoric acids, any salts thereof, for example, monoammonium phosphate, diammonium phosphate, potassium phosphate, and urea phosphate. Petition 870210109957, dated 11 / 26 / 2021, page 15 / 61 8 / 26
[0027] According to a first aspect, a liquid composition is provided comprising phosphorus, molybdenum, cobalt, a solvent and monoethanolamine. The cobalt is present as vitamin B12 and the composition has a pH between 6.0 and 7.5.
[0028] It is known in the field of agriculture to coat seeds with a composition comprising phosphorus, molybdenum, and cobalt. Phosphorus (P) contributes, among other things, to root growth. Coating a seed with an available source of phosphorus ensures that the seed has sufficient phosphorus for the first stage of growth and allows for the growth of strong, long roots that will be able to absorb the necessary nutrients from the soil once the coated nutrients on the seeds are consumed. Molybdenum (Mo) is a micronutrient required by plants and is known to be involved in a variety of biological processes in plants. For example, it is necessary for the synthesis and activity of the enzyme nitrate reductase, which reduces nitrate to nitrogen in the plant. Mo is also essential for the symbiotic nitrogen fixation by the bacterium Rhizobia in root nodules of legumes, which depends on the activity of the bacterial Mo-dependent enzyme, nitrogenase.Cobalt (Co) also plays a role in symbiotic nitrogen fixation and is complementary to Mo. Cobalt is an essential component of vitamin B12, which is involved in various biochemical processes and is vital for the growth and development of Rhizobia bacteria. Seed treatment compositions generally include a cobalt salt or chelate as a cobalt source. However, it is known that... Petition 870210109957, dated 11 / 26 / 2021, page 16 / 61 9 / 26 At least some of the cobalt is converted into vitamin B12. Thus, it was hypothesized that directly supplying vitamin B12 to the seed would increase the efficiency of cobalt nutrition. An important criterion for seed coating compositions, especially legume seeds, is their pH. In fact, legume seeds are often sprayed with a rhizobium inoculant. Rhizobia are bacteria capable of fixing atmospheric nitrogen gas into a form available to plants, for example, ammonium ions, which can subsequently be converted into nitrates, which are even more available to plants than ammonium. Rhizobia prefer a neutral pH environment. A pH between 6.0 and 7.5 is considered ideal for rhizobia growth. Rhizobia need a host to grow and fix nitrogen gas, and it has been observed that they grow particularly well in the root nodules of legumes.Therefore, it has become common practice to inoculate seeds of these crops with the bacteria. This reduces the amount of nitrogen-containing fertilizer that needs to be supplied to the crops and improves soil quality.
[0029] However, liquid compositions containing phosphorus can be quite acidic, especially if the phosphorus source is a phosphoric acid, for example orthophosphoric acid or polyphosphoric acids. And it has been observed that seed treatment compositions containing a source of ammonium, nitrate and / or urea delay or inhibit nodule formation, so it is desirable to avoid compounds containing these nitrogen sources in Petition 870210109957, dated 11 / 26 / 2021, page 17 / 61 10 / 26 composition for seed treatment. Consequently, common sources of phosphorus for fertilizers, such as ammonium phosphate, diammonium phosphate, urea phosphate, and ammonium polyphosphate, are not recommended, even though they have a much higher pKa than phosphoric acid.
[0030] Furthermore, it was observed that solutions containing phosphate salts and molybdate salts, particularly sodium molybdate, were not very stable: precipitation and crystallization were observed. It was found that the pH of the composition can be raised to the desired level by adding monoethanolamine. Monoethanolamine is a small bidentate molecule with a basic site, the primary amine. Adding it to the composition increases the pH, but also stabilizes the phosphate anions. Monoethanolamine does not contain any nitrogen in a form available to plants, therefore no detrimental effect on nodule growth was observed.
[0031] The solvent for the liquid composition may be essentially water, but it may also be a mixture of solvents comprising water and other solvents suitable for use in agriculture, such as alcohols, glycols and their derivatives, such as ethers. The solvent or mixture of solvents must be able to solubilize the different components of the liquid composition at room temperature. In addition, it must present a limited health risk to facilitate its use by farmers or operators. In one embodiment, the solvent is essentially water.
[0032] The liquid composition disclosed here was coated onto seeds of legume crops, and the seeds were dried and sown in the soil. The germination rate increased, Petition 870210109957, dated 11 / 26 / 2021, page 18 / 61 11 / 26 as well as the number of nodules, root mass and the amount of dry matter in the aerial part.
[0033] In one embodiment, the composition comprises from about 10 to about 250 g / l, particularly from about 50 to about 200 g / l of phosphorus, expressed as P2O5. It is advantageous to have a high concentration of phosphorus in the liquid composition. This allows supplying the seed with a large amount of phosphorus without using a large quantity of the composition. However, it is not desirable and / or possible to use a composition with a higher concentration, as this leads to stability problems and may also damage the seeds.
[0034] In one embodiment, the composition comprises from about 10 to about 250 g / l, particularly from about 50 to about 200 g / l, of molybdenum. It is advantageous to have a high concentration of molybdenum in the liquid composition. This allows supplying the seed with a large amount of molybdenum without using a large quantity of the composition.
[0035] In one embodiment, vitamin B12 is present as one or more of cobalamin, cyanocobalamin, hydroxocobalamin, methylcobalamin, and adenosylcobalamin. Vitamin B12 is also called cobalamin and is a metal complex with a cobalt atom at its center surrounded by a corrin ring. A corrin ring is a 15-membered macrocyle comprising a pyrrolidine and three dihydropyrrole rings. The metal complex consisting of the metal and the corrin ring is not stable and requires an additional ligand which may be a cyanide group, a hydroxide, a methyl, or a 5'-deoxyadenosyl. Petition 870210109957, dated 11 / 26 / 2021, page 19 / 61 12 / 26
[0036] In one embodiment, the composition comprises orthophosphoric acid. Orthophosphoric acid is a well-known and suitable source of phosphorus for use in agriculture. It is widely available and well absorbed by plants.
[0037] In one embodiment, the composition comprises a potassium salt of phosphoric acids. The acid may be orthophosphoric acid or a polyphosphoric acid. Potassium is a plant nutrient, so it may be advantageous to supply it to seeds to aid in the initial growth phase. It is compatible with the other components of the liquid composition and does not affect the development of rhizobia.
[0038] In one embodiment, the composition comprises 1 to 45% by weight, particularly 5 to 45%, more particularly 10 to 40%, even more particularly 15 to 40% by weight, of orthophosphoric acid.
[0039] In one embodiment, the composition comprises 1 to 20% by weight of monoethanolamine. In another embodiment, the composition comprises 2 to 20% by weight, particularly 5 to 20%, more particularly 5 to 15%, even more particularly 10 to 15% by weight of monoethanolamine.
[0040] In one embodiment, the weight ratio of monoethanolamine to orthophosphoric acid varies from 1:5 to 1:1, particularly from 1:3 to 1:1. The weight ratio of monoethanolamine to orthophosphoric acid needs to be adjusted to optimize the composition characteristics. If too little monoethanolamine is added, the solution may not be stable enough, the pH may not be high enough, and the rhizobium will not be in ideal conditions. If used Petition 870210109957, dated 11 / 26 / 2021, page 20 / 61 13 / 26 too much monoethanolamine, the cost of the composition will increase unnecessarily, the pH may increase too much and the nutrient content will decrease, as monoethanolamine does not provide any available nutrients to the plants.
[0041] In one embodiment, the composition comprises ethylenediaminetetraacetic acid (EDTA) or any of its salts, particularly tetrasodium ethylenediaminetetraacetate. A risk when preparing an aqueous solution containing phosphate ions is the risk of forming calcium phosphate, which is highly insoluble in water and precipitates. It is preferable to use a water source with a low calcium content to prepare the aqueous composition described herein; however, this may not always be possible depending on the water sources available to farmers. To prevent the precipitation of calcium phosphate, it is possible to add a chelating agent with a strong affinity for calcium. The agent will sequester the calcium present in the water and prevent any formation of calcium phosphate. EDTA is a well-known and widely available chelating agent with a strong affinity for calcium. It is also non-toxic to humans or plants, so it does not introduce any additional precautions for the use of the aqueous composition.Another name for EDTA is 2,2',2'',2'''-(ethane-1,2-diyldinitrile) tetraacetic acid.
[0042] In one embodiment, the composition comprises 0.1 to 1.0% by weight, particularly 0.1 to 0.7% by weight of EDTA.
[0043] In one embodiment, the concentration of vitamin B12 varies from about 125 mg / l to 500 mg / l. It was found that a low amount of vitamin B12 was sufficient to obtain Petition 870210109957, dated 11 / 26 / 2021, page 21 / 61 14 / 26 significant results in agronomic trials. This was surprising, as typical seed treatment compositions contain a much higher Co load (1 to 2% by weight), especially considering the high molecular weight of vitamin B12 compared to the weight of the Co atom. Co represents about 3.7 to 4.4% by weight of vitamin B12.
[0044] In one embodiment, the liquid composition comprises an antifreeze agent, particularly selected from the group of glycerin, monopropylene glycol, monoethylene glycol, sugars, sugar alcohols or mixtures thereof. For reasons of storage stability under varying climatic conditions, it is advantageous to incorporate an antifreeze agent into the seed treatment composition. The antifreeze component must be agriculturally acceptable and have no deleterious effect on rhizobium bacteria. Suitable antifreeze additives are glycerin, monopropylene glycol, monoethylene glycol, sugars and sugar alcohols such as sorbitol.
[0045] In one embodiment, the composition comprises a biostatic agent, particularly selected from the group of sodium propionate, lactic acid and mixtures thereof. In one embodiment, the composition comprises sodium propionate and lactic acid. Liquid compositions as described above have been found to be susceptible to biological deterioration by, for example, mold growth. It is therefore advantageous to incorporate a preservative into the composition. However, the use of typical broad-spectrum biocides such as 2-methyl-2H-isothiazolin-3-one (MIT) or 1,2-benzisothiazol(2H)-one (BIT) is not desirable, as these may have a Petition 870210109957, dated 11 / 26 / 2021, page 22 / 61 15 / 26 deleterious effect on rhizobia bacteria if the composition was co-applied to seeds with inoculant. Therefore, it was found that the use of biostatic agents, such as sodium propionate or lactic acid, are more suitable, as they protect the composition from deterioration but do not destroy rhizobia bacteria when mixed in a co-applied seed treatment.
[0046] In one embodiment, the composition comprises orthophosphoric acid, monoethanolamine, tetrasodium EDTA, sodium molybdate, cyanocobalamin, glycerin, sodium propionate, lactic acid and water.
[0047] In one embodiment, the composition comprises orthophosphoric acid, monoethanolamine, tetrasodium EDTA, sodium molybdate, cyanocobalamin, monopropylene glycol, sodium propionate, lactic acid and water.
[0048] In one embodiment, the composition comprises orthophosphoric acid, monoethanolamine, tetrasodium EDTA, sodium molybdate, cyanocobalamin, monoethylene glycol, sodium propionate, lactic acid and water.
[0049] In one modality, the composition comprises from 1 to 45% by weight orthophosphoric acid, 1 to 20% by weight monoethanolamine, 0.1 to 1.0% by weight tetrasodium EDTA, 1 to 25% by weight sodium molybdate, 0.001 to 1.0% by weight cyanocobalamin, particularly 0.001 to 0.1% by weight cyanocobalamin, 1 to 15% by weight glycerin, 0.01 to 1.0% by weight of sodium propionate, 0.01 to 1.0% by weight of lactic acid, and 40 to 80% by weight of water.
[0050] According to another aspect, a method is provided for preparing a liquid composition comprising phosphorus, Petition 870210109957, dated 11 / 26 / 2021, page 23 / 61 16 / 26 molybdenum, cobalt, and monoethanolamine. The method comprises the steps of providing a phosphorus source, a molybdenum source, a vitamin B12 source, and monoethanolamine; mixing the phosphorus source, the molybdenum source, and the vitamin B12 source with a solvent; and adding an amount of monoethanolamine so that the pH is between 6.0 and 7.5.
[0051] The solvent for preparing the liquid composition may comprise water, but also other solvents suitable for use in agriculture, such as alcohols, glycols, and their derivatives, such as ethers. The solvent or solvent mixture must be able to solubilize the different components of the liquid composition at room temperature. In addition, it must present a limited health risk to facilitate its use by farmers or operators. In one embodiment, the solvent is essentially water.
[0052] In one embodiment, the liquid composition prepared according to the above method comprises from about 10 to about 250 g / l, particularly from about 50 to about 200 g / l of phosphorus, expressed as P2O5.
[0053] In one embodiment, the liquid composition prepared according to the above method comprises from about 10 to about 250 g / l, particularly from about 50 to about 200 g / l, of molybdenum.
[0054] In one embodiment, the source of molybdenum is essentially sodium molybdate.
[0055] In one embodiment, the source of vitamin B12 is selected from the group of cobalamin, cyanocobalamin, hydroxocobalamin, methylcobalamin, adenosylcobalamin and mixtures thereof. Petition 870210109957, dated 11 / 26 / 2021, p. 24 / 61 17 / 26
[0056] In one embodiment, the phosphorus source is selected from the group of orthophosphoric acid, polyphosphoric acid, or any salts thereof. In another embodiment, the phosphorus source is essentially orthophosphoric acid.
[0057] In one embodiment, the amount of monoethanolamine is selected so that the weight ratio of monoethanolamine to orthophosphoric acid in the final composition ranges from 1:5 to 1:1, particularly from 1:3 to 1:1.
[0058] In one embodiment, ethylenediaminetetraacetic acid (EDTA) or any of its salts, particularly tetrasodium ethylenediaminetetraacetate, is supplied and mixed into the solution in step b) described above.
[0059] In one embodiment, an antifreeze agent is provided and mixed into the solution in step b) described above. In one embodiment, the antifreeze agent is selected from the group of glycerin, monopropylene glycol, monoethylene glycol, sugars, sugar alcohols or mixtures thereof.
[0060] In one embodiment, a biostatic agent is provided and mixed into the solution in step b) described above. In one embodiment, the biostatic agent is selected from the group of sodium propionate, lactic acid and mixtures thereof.
[0061] According to another aspect, a method for preparing seeds of legume crops is provided. The method comprises the steps of coating the seeds with the liquid composition described herein, coating the seeds with a composition comprising a rhizobium inoculant, and drying the seeds coated with both compositions. Petition 870210109957, dated 11 / 26 / 2021, page 25 / 61 18 / 26
[0062] Methods for coating seeds with a liquid solution are well known in the field of agriculture. Any known coating method is suitable for carrying out the present method, for example, a rotary seed coater, a drum seed coater, a fluidized bed seed treatment apparatus, or a standard mixing drum. The order of application of the coating is not critical to obtaining the desired technical effect. The composition comprising phosphorus, molybdenum, and vitamin B12 can be coated first, followed by the rhizobium inoculant coating; or it can be coated after the inoculant; or both solutions can be added to the seeds at the same time. The seeds are dried at room temperature, but can also be exposed to a slightly pre-heated air current to accelerate drying.
[0063] In one embodiment, the liquid composition is applied to the seeds at a rate of about 0.5 l per ton to about 5 l per ton. The application rate must be high enough to provide sufficient nutrients to the seeds, but too much may reduce or delay germination. In addition, a high load may make the seeds sticky and difficult to handle and spread.
[0064] In one embodiment, the composition comprising a rhizobium inoculant is applied to the seeds at a rate of about 1 l per ton to about 10 l per ton. Commercial solutions come with a suggested application rate. Small deviations from the recommended rate may be accepted to account for the application of the other liquid composition. Petition 870210109957, dated 11 / 26 / 2021, p. 26 / 61 19 / 26
[0065] In one embodiment, the liquid composition described here is applied directly to the soil before or after planting the seeds.
[0066] According to another aspect, the use of a liquid composition as defined herein is provided for the treatment of legume crop seeds, in particular to improve germination. Example 1
[0067] The following example shows the formulation required to prepare 1 liter of a liquid seed treatment composition containing 125 mg / l of cyanocobalamin. The classes of raw materials used were as follows: Orthophosphoric acid - high purity (food grade); monoethanolamine 90% - made by diluting monoethanolamine 99% high purity; Tetrasodium EDTA - technical grade, assay 86%; Sodium molybdate - disodium molybdate dihydrate of high purity, minimum 39.5% w / w Mo; Cyanocobalamin assay 96% pure crystalline grade; Glycerin - minimum technical grade purity of 99.5%; Sodium propionate - food grade; Lactic acid - food grade 80%. Water: 659.945 g Orthophosphoric acid 75%: 231.25 g Monoethanolamine 90%: 120.45 g Tetrasodium EDTA: 2.60 g Sodium molybdate: 163.50 g Cyanocobalamin: 0.125 g Glycerin: 80.00 g Sodium propionate: 1.00 g Lactic acid 80%: 1.00 g Petition 870210109957, dated 11 / 26 / 2021, p. 27 / 61 20 / 26
[0068] The method used to write the essay was as follows:
[0069] Water (600ml) was placed in a glass container fitted with an impeller stirrer. Under stirring, the components were added in the following order, ensuring complete dissolution of each before proceeding to the next: tetrasodium EDTA, 90% monoethanolamine, 75% orthophosphoric acid, lactic acid, glycerin, sodium molybdate, cyanocobalamin (pre-dissolved in 50 ml of water), sodium propionate. Finally, the remaining water was added to make up a volume of 1 liter.
[0070] The resulting product was a transparent red solution with the following physicochemical characteristics: Density: 1.26 kg / l pH: 6.0-7.0 P2O5 content: 12.5% by weight / v (125 g / l) Mo content: 6.5% by weight / v (65 g / l) Cyanocobalamin: 125 mg / l
[0071] The product remained stable for at least 8 weeks when stored at room temperature, 0°C and 45°C. Example 2
[0072] The following example shows the formulation required to prepare 1 liter of a liquid seed treatment composition containing 250 mg / l of cyanocobalamin. Water: 659.82 g Orthophosphoric acid 75%: 231.25 g Monoethanolamine 90%: 120.45 g Petition 870210109957, dated 11 / 26 / 2021, page 28 / 61 21 / 26 Tetrasodium EDTA: 2.60 g Sodium molybdate: 163.50 g Cyanocobalamin: 0.125 g Glycerin: 80.00 g Sodium propionate: 1.00 g Lactic acid 80%: 1.00 g
[0073] The production method was as described above and the resulting product was a clear red solution with the following physicochemical characteristics: Density: 1.26 kg / l pH: 6.0-7.0 P2O5 content: 12.5% by weight / v (125 g / l) Mo content: 6.5% by weight / v (65 g / l) Cyanocobalamin: 250 mg / l
[0074] The product remained stable for at least 8 weeks when stored at room temperature, 0°C and 45°C. Example 3
[0075] The following example shows the formulation needed to make 1 liter of a liquid seed treatment composition containing 500 mg / l of cyanocobalamin. Water: 659.57 g Orthophosphoric acid 75%: 231.25 g Monoethanolamine 90%: 120.45 g Tetrasodium EDTA: 2.60 g Sodium molybdate: 163.50 g Cyanocobalamin: 0.125 g Glycerin: 80.00 g Sodium propionate: 1.00 g Petition 870210109957, dated 11 / 26 / 2021, page 29 / 61 22 / 26 Lactic acid 80%: 1.00 g
[0076] The production method was as described above and the resulting product was a clear red solution with the following physicochemical characteristics: Density: 1.26 kg / l pH: 6.0-7.0 P2O5 content: 12.5% by weight / v (125 g / l) Mo content: 6.5% by weight / v (65 g / l) Cyanocobalamin: 500 mg / l
[0077] The product remained stable for at least 8 weeks when stored at room temperature, 0°C and 45°C. Example 4
[0078] The seed treatment compositions according to Examples 1, 2, and 3 above were tested for agronomic efficacy against a commercial Co / Mo seed treatment product containing 62.5 g / l of Mo and 12.5 g / l of Co (derived from sodium molybdate and cobalt(II) sulfate). The trial was conducted on soybean (Glycine max), Elena variety, and was arranged in a randomized complete block design with six replications. Each seed treatment composition was co-applied to soybean seed along with liquid rhizobium inoculant (Liquifix, Legume Technology Ltd). An untreated control and a control treated only with inoculant were included in the trial. The following table summarizes the treatments used in the trial: Petition 870210109957, dated 11 / 26 / 2021, pp. 30 / 61 23 / 26 In Treatment Rate of Inoculant Fertilizer Seed Treatment Rate 1 Control 1 - No treatment - - 2 Control 2 - Inoculant only 4 l / MT - 3 Commercial product Co / Mo 62.5 g / l Mo and 12.5 g / l Co 4 l / MT 2 l / MT 4 Example 1 Composition - 125 mg / l B12 4 l / MT 2 l / MT 5 Example 2 Composition - 250 mg / l B12 4 l / MT 2 l / MT 6 Example 3 Composition - 500 mg / l B12 4 l / MT 2 l / MT
[0079] The treated seed was left to air dry overnight before being sown in 5 cm pots filled with a growing medium composed of a 1:1 mixture of vermiculite and perlite (one seed per pot). The pots were watered with deionized water and placed in a growth chamber. Watering with deionized water continued during the germination period. After emergence, the plants were watered with nitrogen-free nutrient solution (pH 6.0). The germination rate was evaluated 7 days after sowing, and the plants were harvested 21 days after sowing to record the number of nodules formed. The results are shown in the following table: In Treatment Germination Rate (7 days after sowing) 1 Control 1 - No treatment 83.3% 2 Control 2 - Inoculant only 72.2% 3 Commercial Co / Mo product 83.3% 4 Example 1 100% 5 Example 2 100% 6 Example 3 88.9% Petition 870210109957, dated 11 / 26 / 2021, pp. 31 / 61 24 / 26 Example 5
[0080] The seed treatment compositions according to Examples 1 and 3 above were further tested for agronomic efficacy in comparison with a commercial Co / Mo seed treatment product containing 62.5 g / l Mo and 12.5 g / l Co (derived from sodium molybdate and cobalt(II) sulfate). The trial was conducted on soybean (Glycine max), variety Silverka, and was arranged in a randomized complete block design with 4 replications. Each seed treatment composition was co-applied to soybean seed along with liquid rhizobium inoculant (Liquifix, Legume Technology Ltd). An untreated control and a control treated only with inoculant were included in the trial. The following table summarizes the treatments used in the trial: In Treatment Rate of Inoculant Seed Fertilizer Treatment Rate 1 Control 1 - No treatment - - 2 Control 2 - Inoculant only 4 l / MT - 3 Commercial product Co / Mo 62.5 g / l Mo and 12.5 g / l Co 4 l / MT 2 l / MT 4 Example 1 - 125 mg / l of Cyanocobalamin 4 l / MT 2 l / MT 5 Example 3 - 125 mg / l of Cyanocobalamin 4 l / MT 2 l / MT
[0081] The treated seed was left to air dry overnight before being sown in 20 cm pots filled with washed sand (5 seeds per pot). The pots were placed in a climate-controlled greenhouse and watered with deionized water for 7 days during the germination period. After emergence, the plants were watered with nutrient solution. Petition 870210109957, dated 11 / 26 / 2021, pp. 32 / 61 25 / 26 nitrogen-free soil (pH 6.0, containing half the optimum concentrations of P, K, Mg, Ca, B, Cu, Fe, Mn, Mo, Zn). Plants were visually assessed and harvested 6 weeks after sowing. Root systems were rinsed with water to remove adhering sand, and the following parameters were recorded: number of nodules, root dry mass, shoot dry mass. Nodules were examined to assess whether they were active by cutting them open (pink tissue coloration was considered an indication that they were active). The results are shown in the table below: Treatment Number of nodules (per pot) Root dry matter (per pot) (g) Shoot dry matter (per pot) (g) 1 Control 1 - No treatment 0.00 2.78 3.11 2 Control 2 - Inoculant only 88.25 1.92 4.55 3 Commercial product Co / Mo 62.5 g / l Mo and 12.5 g / l Co 96.25 2.94 4.88 4 Example 1 - 125 mg / l of Cyanocobalamin 101.25 4.26 5.43 5 Example 3 - 125 mg / l of Cyanocobalamin 118.00 3.72 4.61
[0082] Visual observation of the plants at harvest time showed that those without any treatment were very small and stunted, with yellow leaves; plants treated with inoculant only showed better growth and development than untreated control plants, but were smaller and less developed than those in Treatments 3, 4 and 5, which were larger and greener. Petition 870210109957, dated 11 / 26 / 2021, pp. 33 / 61 26 / 26 It was observed that the plant nodules in treatments 3, 4, and 5 were pink when open, indicating that they were active. It was also observed that the plant nodules in treatments 4 and 5 were generally larger in size than those in treatment 3, suggesting earlier nodule establishment in treatments 4 and 5.
Claims
1. A liquid composition comprising phosphorus, cobalt, and a solvent, in which cobalt is present as vitamin B12 and the composition has a pH between 6.0 and 7.5, characterized by: the composition comprising monoethanolamine and 10 to 250 g / l of molybdenum.
2. Liquid composition according to claim 1, characterized in that: the solvent is water.
3. Liquid composition, according to claims 1 or 2, characterized by: comprising from 10 to 250 g / l of phosphorus, expressed as P2O5.
4. Liquid composition, according to claim 3, characterized in that the composition comprises from 50 to 200 g / l of phosphorus, expressed as P2O5.
5. Liquid composition, according to claims 1 to 4, characterized by: comprising 50 to 200 g / l of molybdenum.
6. Liquid composition according to claims 1 to 5, characterized by: comprising sodium molybdate as a source of molybdenum.
7. Liquid composition, according to claims 1 to 6, characterized in that: vitamin B12 is present as one or more of cobalamin, cyanocobalamin, hydroxocobalamin, methylcobalamin and adenosylcobalamin. Petition 870260027410, dated 03 / 23 / 2026, page 10 / 13 2 / 4 8. Liquid composition according to claims 1 to 7, characterized by: comprising orthophosphoric acid.
9. Liquid composition according to claim 8, characterized in that: the weight ratio of monoethanolamine to orthophosphoric acid varies from 1:5 to 1:
1.
10. Liquid composition according to claim 9, characterized in that: the weight ratio of monoethanolamine to orthophosphoric acid varies from 1:3 to 1:
1.
11. Liquid composition, according to claims 1 to 10, characterized by: comprising ethylenediaminetetraacetic acid (EDTA) or any of its salts.
12. Liquid composition according to claim 11, characterized by: comprising tetrasodium ethylenediaminetetraacetate.
13. Liquid composition, according to claims 1 to 12, characterized by: the concentration of vitamin B12 varying from 125 mg / l to 500 mg / l.
14. Liquid composition according to claims 1 to 13, characterized by: comprising an antifreeze agent.
15. Liquid composition, according to claim 14, characterized by: Petition 870260027410, dated 03 / 23 / 2026, page 11 / 13 3 / 4 comprising an antifreeze agent selected from the group of glycerin, monopropylene glycol, monoethylene glycol, sugars, sugar alcohols or mixtures thereof.
16. Liquid composition, according to claims 1 to 15, characterized by: comprising a biostatic agent.
17. Liquid composition according to claim 16, characterized by: comprising a biostatic agent selected from the sodium propionate and lactic acid group.
18. Method of preparing the liquid composition, as defined in any of claims 1 to 17, characterized by: comprising the steps of: a) providing a source of phosphorus, a source of molybdenum, a source of vitamin B12 and monoethanolamine; b) mixing the source of phosphorus, the source of molybdenum and the source of vitamin B12 with a solvent; and c) adding an amount of monoethanolamine such that the pH is between 6.0 and 7.5; wherein the resulting liquid composition comprises from 10 to 250 g / m2 of molybdenum.
19. Method for preparing legume crop seeds, characterized by: comprising the steps of: a) coating the seeds with the liquid composition as defined in any one of claims 1 to 12; b) coating the seeds with a composition comprising a rhizobium inoculant; and Petition 870260027410, dated 03 / 23 / 2026, p. 12 / 13 4 / 4 c) drying the seeds coated with both compositions.
20. Method according to claim 19, characterized in that: in step a), the liquid composition is applied to the seeds at a rate of 0.5 l per ton to 5 l per ton.
21. Use of the liquid composition, as defined in any one of claims 1 to 16, characterized in that: it is applicable for the treatment of legume seeds, particularly to improve germination.