System, method and use of ionized air to sanitize an object

Description

1 / 26 SYSTEM, METHOD AND USE OF IONIZED AIR TO SANITIZE AN OBJECT Field of Technique

[0001] This disclosure relates to a system, method and use of ionized air to sanitize an object. In particular, this disclosure relates to a system, method and use of free radicals to sanitize the surface of an object in a container. For example, in some embodiments, the container may be a toy box, or the system may be used to sanitize medical instruments, shared items and / or pet equipment. Fundamentals of the Invention

[0002] Bacteria are microscopic living cells with a relatively simple structure; they are unicellular. Bacterial infections are contracted through the respiratory and digestive tracts or through bites, scratches, and cuts. Ambient air bacteria come from two sources: aerosols generated by water, such as humidifiers, air conditioners, cooling towers, dirty water, etc., and aerosols generated by humans or animals. Saprophytic and pathogenic bacteria are dispersed in the air by humans when they sneeze, cough, and talk. These bacteria can survive for varying periods, the duration of which depends on the size of the projected droplets, the air temperature, the relative humidity, and the presence of a substrate that allows their propagation.

[0003] The classification of bacteria is based on cellular, morphological, or biochemical characteristics. They are divided into two large groups, depending on their reaction to Gram staining (Gram-positive or Gram-negative bacteria). Bacteria need a lot of moisture to develop. Gram-negative bacteria have a fragile cell wall that does not tolerate dehydration suffered during prolonged exposure to air or during sample collection. Gram-positive bacteria have a more resistant cell wall, and some produce spores that give them greater resistance to varying environmental conditions. This group includes thermophilic bacteria, whose growth is favored by higher temperatures.

[0004] In outdoor environments, bacteria mainly come from water, soil, and plants, and are associated with the presence of humans and animals. Bodies of water can aerosolize them into the air, as can emissions from some processes. Petition 870260068441, dated 10 / 07 / 2026, page 5 / 33 2 / 26 industrial and refrigeration units. Inside buildings, bacteria originate mainly from the occupants, as they constitute the natural flora of the skin and mucous membranes. Frequently, their species are more numerous and their concentrations higher than those in the external environment.

[0005] Peptidoglycans are components of the bacterial cell wall. They are suspected to be potential agents of pulmonary inflammation associated with the inhalation of Gram-positive bacteria. Exotoxins are bioactive molecules, usually proteins secreted during bacterial growth. They are also released during bacterial lysis. Although generally associated with infectious diseases such as botulism, cholera, and tetanus, they can be found in substrates that favor bacterial growth and subsequently take the form of aerosols. The risks associated with their presence in the air are not documented.

[0006] Most bacteria found naturally in humans do not cause adverse health effects. Some are even essential to both the human body and the environment. Health risks arise when concentrations of certain species become abnormally high. Thus, high concentrations of thermoactinomycete bacteria can cause hypersensitivity pneumonitis, such as farmer's lung disease. Certain bacteria are recognized as responsible for infectious diseases. The health risk associated with the presence of Legionella pneumophila bacteria, or legionellosis, is well documented. There are two distinct forms of legionellosis: Legionnaires' disease, a progressive pneumonia that can be fatal, and Pontiac fever, which causes flu-like symptoms. This bacterium is known for its ability to thrive in water reservoirs. It is prone to drying out and does not survive outside of water.However, it can be transmitted through the air via the projection of water droplets containing it. The genus Mycobacterium is also of interest to health, and particularly the species Mycobacterium tuberculosis, the causative agent of tuberculosis. Most mycobacterial species live in soil and water, but they are also found in diseased tissues of warm-blooded animals, including humans. Mycobacterium tuberculosis bacteria are airborne via droplets generated by carriers of the disease and through ventilation systems. Petition 870260068441, dated 10 / 07 / 2026, p. 6 / 33 3 / 26

[0007] Viruses are submicroscopic infectious agents that replicate within the living cells of an organism. Outside of an infected cell, viruses exist as independent particles, or virions. Like bacteria, viruses can also be dispersed in the air by humans when they sneeze, cough, and talk. These viruses can survive for varying periods, the duration of which depends on the size of the projected droplets, air temperature, relative humidity, and the presence of a substrate that allows their propagation.

[0008] Both bacteria and viruses can also survive on the surface of objects for varying periods. This increases the likelihood of transmission when the object is handled by different users.

[0009] Children's toys, especially in a shared environment such as a daycare or kindergarten classroom, can be effective transmitters of bacteria and viruses from one child to another. Other objects that may be shared between individuals can also be effective transmitters of bacteria and viruses between users, including medical instruments, shared living / office space items, and pet equipment, among others. Summary of the Invention

[0010] In several examples, the present disclosure describes systems, methods and uses of ionized air to sanitize the surface of an object in a compartment.

[0011] In some examples, the present disclosure describes a system for sanitizing the surface of an object, the system comprising: a first compartment having a first opening sized to receive the object through it, and having a first cover sized to cover the first opening, the first cover being configured to be resealable over the first opening of the first compartment, and an air ionizer coupled to the first compartment to generate free radicals for discharge into the first compartment, wherein the reaction of the free radicals with microorganisms on the surface of the object inactivates the microorganisms.

[0012] In some examples, the present disclosure describes a method for sanitizing the surface of an object, the method comprising: placing the object in a first compartment through a first opening sized to receive the object through it; sealing the first opening of the first compartment with a Petition 870260068441, dated 10 / 07 / 2026, page 7 / 33 4 / 26 resealable cover, and generate free radicals with an air ionizer and discharge the free radicals into the first compartment, where the reaction of the free radicals with microorganisms on the surface of the object inactivates the microorganisms.

[0013] In some examples, the present disclosure describes the use of free radicals to sanitize the surface of an object, the use comprising: generating and discharging the free radicals in a sealed compartment containing the object, the compartment having a resealable cover, wherein the reaction of the free radicals with microorganisms on the surface of the object inactivates the microorganisms.

[0014] In any of the above examples, the air ionizer is a cold plasma emitter and the free radicals comprise hydroxyl radicals.

[0015] In either of the above examples, a fan is attached to the first compartment, the fan configured to agitate the air within the first compartment.

[0016] In any of the above examples, the air ionizer is configured to generate free radicals at a rate of at least 400x106 molecules / cm3.

[0017] In any of the examples above, the first compartment has a volume of 150 L or less, a volume of 80 L or less, or a volume of 25 L or less.

[0018] In any of the examples above, the concentration of free radicals within the first compartment is maintained between 300,000 and 25,000,000 molecules / cm3.

[0019] In either of the examples above, the air ionizer is positioned inside the first compartment to generate free radicals from the air inside the first compartment.

[0020] In either of the above examples, a second compartment is in fluid communication with the first compartment, the fan being positioned between the first and second compartments to direct air from one compartment to the other.

[0021] In either of the above examples, the air ionizer is attached to the second compartment to generate free radicals in it, the fan being positioned between the first and second compartments to discharge the free radicals from the second compartment into the first compartment. Petition 870260068441, dated 10 / 07 / 2026, page 8 / 33 5 / 26

[0022] In either of the above examples, an air channel is coupled to the first compartment, where one end of the air channel is fluidly coupled to the first compartment and an opposite end of the air channel is fluidly coupled to the second compartment.

[0023] In any of the examples above, the volume of the second compartment is less than the volume of the first compartment.

[0024] In either of the above examples, a second opening is positioned in the second compartment and a second cover is sized to cover the second opening, the second cover being configured to be resealable over the second opening of the second compartment.

[0025] In any of the above examples, the first compartment comprises a plastic storage box and the first cover is a plastic cover that is resealable over the first opening with a snap-fit ​​mechanism.

[0026] In any of the examples above, the first compartment is a toy box set up for storing children's toys.

[0027] In any of the examples above, the system is used to sanitize health instruments, shared objects and / or pet equipment. Brief Description of the Drawings

[0028] By way of example, reference will be made to the attached drawings, which show illustrative embodiments of the present patent application, in which: Figure 1 is a front view of a system for sanitizing the surface of an object, according to an example in this disclosure; Figure 2 is a rear perspective view of the system in Figure 1; Figure 3 is an enlarged view of part A of Figure 1; Figure 4 is an enlarged plan view of part B of Figure 3; Figure 5 is a flowchart illustrating a method for cleaning the surface of an object, according to an example in this disclosure; Figure 6 is a plan view of various test surfaces before placement in a compartment; Petition 870260068441, dated 10 / 07 / 2026, page 9 / 33 6 / 26 Figure 7 is a front perspective view of the various test surfaces placed in the lower compartment of the system in Figure 1 in Phase 1 of an exemplary study; Figure 8 is a plan view of the various test surfaces in Figure 7; Figure 9 is a front view of the system in Figure 7; Figure 10 is a partial side view of the system from Figure 1 in a second study; Figure 11 is a front perspective view of several test surfaces placed in the upper compartment of the system in Figure 1 in Phase 2 of the exemplary study; Figure 12 is a partial rear view of an ionizer and a fan in an alternative system for sanitizing the surface of an object used in Phase 3 of the exemplary study; Figure 13 is a rear perspective view of the ionizer and fan shown in Figure 12; Figure 14 is a front perspective view of the ionizer and fan shown in Figure 12; Figure 15 is a plan view of the ionizer and fan shown in Figure 12; Figure 16 is a side view of the ionizer and fan of Figure 12 with a curved rubber wall positioned adjacent to the ionizer; Figure 17 is a top perspective view of the system for sanitizing the surface of an object, according to another example in this description, with internal components illustrated by dashed lines; Figure 18 is a plan view of the system in Figure 17; Figure 19 is a front view of the system in Figure 17; Figure 20 is a right side view of the system in Figure 17; Figure 21 is a cross-sectional view along line CC of Figure 18; Figure 22 is a cross-sectional view along line DD of Figure 19; Petition 870260068441, dated 10 / 07 / 2026, page 10 / 33 7 / 26 Figure 23 is a cross-sectional view along line EE of Figure 19; Figure 24 is a partial cross-sectional view along line GG of Figure 23; Figure 25 is a cross-sectional view along line FF of Figure 19, and Figure 26 is an enlarged view of portion H of Figure 25.

[0029] Similar reference numbers may have been used in different figures to indicate similar components. Description of Illustrative Modalities

[0030] With reference to Figures 1 to 4 and 17 to 26, embodiments of a system 10 for sanitizing the surface of an object are presented, according to the examples in this disclosure. The sanitizing system 10 generally comprises a first compartment 12 and an air ionizer 14 coupled to the first compartment 12.

[0031] The first compartment 12 has a first opening that is shaped and dimensioned to receive one or more objects through it. In particular, the first compartment 12 comprises a first container 18 and a corresponding first cover 16. The first container 18 defines the first opening, and the first cover 16 is dimensioned to cover the first opening. The first cover 16 is additionally configured to be resealable over the first opening of the first compartment 12.

[0032] The first compartment 12 of this disclosure can be designed to be portable, such as having a volume of 150 liters or less. In the embodiment illustrated in Figures 1 and 2, the first compartment 12 has a volume of 80 liters. In alternative applications, the first compartment 12 may have a smaller volume, such as 25 liters or less. The first compartment 12 may also alternatively have a volume greater than 150 liters.

[0033] In the embodiment shown in Figures 1 and 2, the first container 18 is a plastic box 19 and the first cover 16 is a corresponding plastic lid 20 that is resealable over the first opening with a mechanism of Petition 870260068441, dated 10 / 07 / 2026, p. 11 / 33 8 / 26 snap-fit. The plastic lid 20 and the plastic box 19 collectively form a plastic-on-plastic seal between them, sufficient to reduce, if not eliminate, the escape of free radicals from the interior. The plastic box 19 and the corresponding plastic lid 20 may be a standard plastic storage box that is commercially available to the public. In other applications, the first container 18 and the first cover 16 may be made of other materials and / or may use other resealable seals between them.

[0034] For example, when the sanitizing system 10 is used as a toy box, the first container 18 and the first cover 16 can be configured to open from the inside for safety reasons. In this sense, instead of the snap-fit ​​mechanism, the first cover 16 can be weighted so that the weight of the first cover 16 is sufficient to create a seal between the first cover 16 and the first compartment 12 when the first cover 16 covers the first opening of the first compartment 12. In a further application, the first cover 16 and the first compartment 12 can have embedded magnets to assist in aligning and securing the first cover 16 over the first opening of the first compartment 12. To this end, magnets can be positioned around the first opening of the first compartment 12 and other magnets can be positioned correspondingly near the perimeter of the first cover 16.Alternatively, one of the first cover 16 and the first compartment 12 may have embedded magnets, while the other may have ferromagnetic components positioned to match the magnets in use. An advantage in both cases is that the first cover 16 can be opened from the inside. Other known variations of the technique are possible.

[0035] In certain applications, for example, if the air pressure inside the first compartment 12 is greater than the ambient air pressure outside the first compartment 12, the first cover 16 and / or the first container 18 may have a more robust seal. Such a robust seal may involve a gasket (not shown) to make the first compartment 12 airtight when in use.

[0036] Air ionizer 14 is coupled to the first compartment 12 to generate free radicals for discharge into the first compartment 12. The radical reaction Petition 870260068441, dated 10 / 07 / 2026, page 12 / 33 9 / 26 free with microorganisms (including bacteria and viruses) on the surface of the object inactivates the microorganisms, making them unable to function and therefore leading to their inability to spread.

[0037] The air ionizer 14 may be a cold plasma ionization emitter or other ion generator known in the art. The air ionizer 14 is configured to cause the formation of free radicals, particularly hydroxyl radicals, which react with microorganisms and deactivate them, as noted above. The cold plasma ionization emitter may be capable of generating 400x106 molecules / cm3 or more. In applications where the first compartment 12 has a very large volume, such as greater than 150 liters, a larger air ionizer 14, or more than one air ionizer 14, can be used to generate the free radicals to fill the first compartment 12. Depending on the size or volume of the first compartment 12, the cold plasma ionization emitter would be configured or selected to generate and maintain the ion concentration within the first compartment 12 at (preferably) 300,000 to 25,000,000 molecules / cm3.

[0038] In some applications, not shown, the air ionizer 14 may be positioned within the first compartment 12 to generate free radicals from the air within the first compartment 12. The sanitizing system 10 may additionally have a fan 22 attached or simply positioned within the first compartment 12, wherein the fan 22 is configured to agitate the air within the first compartment 12. Agitating the air within the first compartment 12 may assist in the contact of a greater number of hydroxyl radicals with the microorganisms on the object surface when the sanitizing system is in use. In some embodiments, one or more objects or walls may be inserted into the first compartment 12 to alter the airflow within the sanitizing system 10. This may influence the distribution of radicals through the first compartment 12 / sanitizing system 10.For example, as shown in Figure 16, a curved wall formed from a sheet of rubber can be positioned next to and above the air ionizer 14 to alter the air agitation patterns in the sanitizing system 10, for example, helping to direct the radicals to the surfaces to be sanitized. Petition 870260068441, dated 10 / 07 / 2026, page 13 / 33 10 / 26

[0039] The sanitization system 10 may additionally include a second compartment 24 coupled to the first compartment 12 in fluid communication. The first compartment 12 may be fluidically coupled to the second compartment 24 by means of a first conduit 25 positioned between them. In the embodiment illustrated in Figures 1 and 2, the first conduit 25 is formed by corresponding holes in the upper and lower parts of the first and second compartments 12 and 14 (respectively). In this case, the fan 22 may be positioned between the first and second compartments 12 and 24, within the first conduit 25, to direct air from one compartment to the other (as shown in Figures 3 and 4). The air ionizer 14 is also shown attached to the second compartment 24 to generate free radicals within the second compartment 24.Since the fan 22 is positioned between the first and second compartments 12 and 24, it can be operated to direct or discharge free radicals from the second compartment 24 into the first compartment 12. In this way, objects placed in the first compartment 12 will not come into contact with the air ionizer 14 in the second compartment 24.

[0040] If the sanitization system 10 includes the second compartment 24, the sanitization system 10 may additionally have an air channel 26, wherein one end of the air channel 26 is fluidically coupled to the first compartment 12 by means of a second conduit 29, and an opposite end of the air channel 26 is fluidically coupled to the second compartment 24. If the fan 22 is operating to direct and discharge free radicals from the second compartment 24 into the first compartment 12, the presence of the air channel 26 allows air from the first compartment 12 to be circulated back into the second compartment 24 for reuse. If the fan 22 is operating in the opposite direction, the air channel 26 allows the free radicals generated in the second compartment 24 to be directed and discharged into the first compartment 12. The fan 22 then directs the air from the first compartment 12 to the second compartment 24 for reuse.Air channel 26 can be any passage that fluidically connects the first compartment 12 to the second compartment 24. In the embodiment illustrated in Figures 1 and 2, the air channel is a hose. Petition 870260068441, dated 10 / 07 / 2026, page 14 / 33 11 / 26

[0041] In some applications, the second compartment 24 may function solely to contain the air ionizer 14, within which free radicals may be generated before being discharged into the first compartment 12. In other applications, the second compartment 24 may also be used to contain an object whose surface needs to be sanitized. In this case, the second compartment 24 may comprise a second container 27 that forms a second opening, and a second cover 28 that is sized to cover the second opening. Similar to the first cover 16 with the first compartment 12, the second cover 28 may be configured to be resealable over the second opening of the second compartment 24. In the embodiment shown in Figures 1 to 4, the second container 27 comprises another plastic box 30, and the second cover 28 is another corresponding plastic lid 32 that is resealable over the second opening with a snap-fit ​​mechanism.The other plastic lid 32 and the other plastic box 30 can collectively form another plastic-on-plastic seal between themselves, which is sufficient to reduce, if not eliminate, the escape of free radicals from the interior. The other plastic box 30 and the corresponding other plastic lid 32 can also be a standard plastic storage box that is commercially available to the public.

[0042] Similar to the first container 18 and the first cover 16 mentioned above, the second container 27 and the second cover 28 may be made of other materials and / or may use other resealable seals between them.

[0043] The volume of the second compartment 24 may be different, such as smaller, than the volume of the first compartment 12. In the embodiment illustrated in Figures 1 to 4, the volume of the second compartment 24 is 24 L, while the volume of the first compartment 12 is 80 L. When the volume of the second compartment 24 is different from that of the first compartment 12, this naturally creates different concentrations of free radicals in the respective compartments. When the same air ionizer is used for the same period of time, a smaller volume tends to have a higher concentration of free radicals, while a larger volume tends to have a lower concentration of free radicals. Both the first (larger) compartment 12 and the second (smaller) compartment 24 can be used simultaneously to accommodate an object whose surface needs to be sanitized. Such Petition 870260068441, dated 10 / 07 / 2026, page 15 / 33 The 12 / 26 configuration creates different regions with varying ionization intensities, which can provide the user with the option to sanitize smaller objects more quickly in the second (smaller) compartment 24 and / or sanitize larger objects more slowly in the first (larger) compartment 12.

[0044] Returning to Figures 17 to 26, another embodiment of the system 10 is shown for sanitizing the surface of an object. Similar to the embodiment presented in Figures 1 to 4, this sanitizing system 10 also comprises the first compartment 12, the second compartment 24, and the air ionizer 14. The first compartment 12 includes the first container 18 with a corresponding first cover 16 covering the first opening of the first container 18. In the embodiment presented in Figures 17 to 26, the first cover 16 is attached to the first container 18 with hinges.

[0045] Unlike the embodiment shown in Figures 1 to 4, the first cover 16 and the first container 18 shown in Figures 17 to 26 are not configured for engagement with a snap-fit ​​mechanism. For example, when the sanitizing system 10 is used as a toy box, the first container 18 and the first cover 16 can be configured to be opened from the inside, for safety reasons. In this sense, the first cover 16 can be weighted so that the weight of the first cover 16 is sufficient to create a temporary seal between the first cover 16 and the first compartment 12, when the first cover 16 covers the first opening of the first compartment 12.

[0046] In a further application, the first cover 16 and the first compartment 12 may have embedded magnets to assist in aligning the first cover 16 over the first opening of the first compartment 12 to create the temporary seal between them. To this end, magnets (not shown) may be positioned around the first opening of the first compartment 12 and other magnets (not shown) may be positioned correspondingly near the perimeter of the first cover 16. Alternatively, one of the first cover 16 and the first compartment 12 may have embedded magnets, while the other may have ferromagnetic components (not shown) positioned to correspond to the Petition 870260068441, dated 10 / 07 / 2026, page 16 / 33 13 / 26 magnets in use. In both cases, the hinges help maintain alignment between the first cover 16 and the first compartment 12.

[0047] One advantage of the examples described above is that the first cover 16 can be opened from the inside. Other variations of resealable seals known in the art can be used.

[0048] Although the air ionizer 14 is also positioned within the second compartment 24, the second compartment in the illustrated embodiment is not configured to contain other objects to be sanitized. Instead, the second compartment 24 in the embodiment of Figures 17 to 26 is sized to contain only the air ionizer 14. And, instead of being formed by two containers (as illustrated in Figures 1 to 4), the first and second compartments 12 and 24 in the embodiment of Figures 17 to 26 are defined within a unitary housing 33. Although the unitary construction allows for a more robust product, the first and second compartments 12 and 24 can, in alternative applications, be removablely attached to each other.

[0049] The embodiment shown in Figures 17 to 26 represents the air ionizer 14 to be positioned directly adjacent to the first compartment 14. To this end, the present embodiment additionally has an ionizer cover 34 positioned to cover the ionizer 14 and to separate the first compartment 12 from the second compartment 24. The ionizer cover 34 can be removablely attached to the first compartment 12 of the housing 33, so that the air ionizer 14 can be accessed from inside the first compartment 12.

[0050] As noted above, the first compartment 12 is fluidically coupled to the second compartment 24 by means of the first conduit 25 positioned between them. As best observed in Figures 21 and 25, the first conduit 25 in the illustrated embodiment comprises multiple openings positioned within the ionizer cover 34 adjacent to the air ionizer 14. Because the ionizer cover 34 is positioned between the first and second compartments 12 and 24, the first conduit 25 allows air to circulate freely in any direction.

[0051] Compartments 12 and 24 are also fluidically coupled by air channel 26, wherein one end of air channel 26 is fluidically coupled to Petition 870260068441, dated 10 / 07 / 2026, page 17 / 33 14 / 26 first compartment 12 by means of the second conduit 29, and the opposite end of the air channel 26 is fluidically coupled to the second compartment 24 in fluid communication with the air ionizer 14 and positioned close to it. Instead of a hose, the air channel 26 in the embodiment shown in Figures 17 to 26 is a passage formed within the unit housing 33 that fluidically connects the first compartment 12 to the second compartment 24.

[0052] Preferably, the first conduit 25 and the second conduit 29 are coupled to the first compartment 12, spaced apart from each other. For example, in the embodiment shown in Figure 17, the first conduit 25 and the second conduit 29 are positioned on opposite sides of the first compartment 12. Furthermore, the first conduit 25 is positioned near the top of the first compartment 12 and the second conduit 29 is positioned near the bottom of the first compartment 12. In this way, air laden with free radicals from one conduit is encouraged to pass through the first compartment 12 (and thus come into contact with the objects contained therein) before being redirected back by the other conduit to the air ionizer 14 for further ionization.

[0053] The fan 22 of the embodiment of Figures 17 to 26 is positioned between the second conduit 29 and the air channel 26, also within the housing 33. Similar to the first embodiment, if the fan 22 is operating to direct and discharge free radicals from the second compartment 24 directly into the first compartment 12 (through the first conduit 25 / openings in the ionizer cover 34), the presence of the air channel 26 allows air from the first compartment 12 to be circulated back to the second compartment 24 for reuse. If the fan 22 is operating in the opposite direction, the air channel 26 allows the free radicals generated in the second compartment 24 to be directed and discharged into the first compartment 12 via the second conduit 29. The air from the first compartment 12 can then enter the second compartment 24 via the first conduit 25 / openings in the ionizer cover 34 for reuse.

[0054] Fan 22 may be located inside housing 33, near the exterior of housing 33, where there is an opening for the fan in housing 33 to allow access to fan 22 from the outside. For this purpose, the embodiment shown Petition 870260068441, dated 10 / 07 / 2026, p. 18 / 33 15 / 26 in Figures 17 to 26 may additionally comprise a fan cover 36 positioned over the fan 22. The fan cover 36 may be removablely attached to the housing 33, so that the fan 22 can be accessed from the outside, for maintenance purposes, for example. The embodiment of the Figures

[0055] 17 to 26 also includes multiple protrusions or projections 38 that extend from the base of the first compartment 12 into the compartment. The protrusions 38 may be attached to the first compartment 12 or may be of unitary construction with the housing 33. As best observed in Figures 19 and 26, the protrusions 38 shown have a cylindrical shape with a conical top. In other applications, the protrusions 38 may have other shapes, such as rectangular, oval, or asymmetrical. The presence of the protrusions 38 at the base of the first compartment 12 allows objects, or at least parts of objects, placed inside the first compartment 12 to be raised from the floor and allow air to pass underneath. In this way, the lower surfaces of these objects can come into contact with the free radicals inside the first compartment when the system 10 is used, thus allowing the lower surfaces of these objects to also be sanitized.

[0056] Returning to Figure 5, Figure 5 is a flowchart illustrating an exemplary method 500 for sanitizing the surface of an object. The exemplary method 500 can be performed using the sanitization system 100, as described above, for example.

[0057] In 502, the object to be sanitized is placed in a first compartment through a first opening sized to receive the object through it. The first compartment can be the first compartment 12, as described above.

[0058] In 504, the first opening of the first compartment is sealed with a resealable cover, such as the first cover 16. The seal between the first compartment and the resealable cover does not need to be airtight. If the first compartment is a plastic box and the first cover is a corresponding plastic lid, the plastic lid can be snapped onto the first opening of the plastic box, wherein the plastic-on-plastic seal between the box Petition 870260068441, dated 10 / 07 / 2026, page 19 / 33 The 16 / 26 plastic and the plastic lid are sufficient to reduce, if not eliminate, the escape of free radicals from the interior.

[0059] In alternative applications, the first compartment can be sealed with the first cover in a different way. For example, in 506, if the first cover 16 is weighted, then the first cover 16 only needs to be placed over the first opening of the first compartment 12 to create the temporary seal between them. Similarly, if the first cover 16 and the first compartment 12 have ferromagnetic components or corresponding magnets incorporated, then the first cover 16 also needs to be simply aligned and placed over the first opening of the first compartment 12 to create the temporary seal between them. An advantage in both cases is that the first cover 16 can be opened from the inside.

[0060] In other applications, if the air pressure inside the first compartment is greater than the ambient air pressure outside the first compartment, the first cover and / or the first compartment may have a more robust seal. Such a robust seal may involve a gasket to make the first compartment 12 airtight.

[0061] In 508, free radicals are generated with an air ionizer, such as a cold plasma emitter, and discharged into the first compartment. Free radicals may include hydroxyl radicals. Free radicals can be generated at a rate of 400 x 10⁶ molecules / cm³ or more by the air ionizer. As noted above, the reaction of free radicals with microorganisms (including bacteria and viruses) on the object surface inactivates the microorganisms. Free radicals can be generated and discharged into the first compartment in several ways.

[0062] In one application, the object to be sanitized and the air ionizer can be placed / positioned inside the first compartment. The first compartment can have a volume of 150 L or less. In 510, free radicals can be generated from the air inside the first compartment, which also contains the object to be sanitized. Optionally, in 512, the air / free radicals inside the first compartment can be agitated to stimulate a greater number of free radicals to react with the microorganisms on the surface of the object. Petition 870260068441, dated 10 / 07 / 2026, page 20 / 33 17 / 26

[0063] In another application, the object to be sanitized can be placed in the first compartment, while the air ionizer can be positioned inside a second compartment fluidically coupled to the first compartment. In this case, in 514, free radicals can be generated in the second compartment and, in 516, free radicals can be directed from the second compartment to the first compartment, where the object to be sanitized is placed. Free radicals can be directed from the second compartment to the first compartment with a fan. The fan can be positioned and secured between the compartments.

[0064] For 510 to 516, free radicals can be generated in such a way that the concentration of free radicals within the first compartment can be maintained between 300,000 and 25,000,000 molecules / cm3

[0065] If the system includes the second compartment, optionally, the other object to be sanitized may alternatively or additionally be placed in the second compartment. The free radicals generated in the second compartment may react with the microorganisms present on the surface of the object in the second compartment in the same manner described above.

[0066] Furthermore, if the system includes the second compartment, in 518, the air from the first compartment can be recirculated back to the second compartment for reuse. To this end, the air from the first compartment can be directed directly back to the second compartment via a conduit or indirectly directed back to the second compartment via an air duct. Thus, method 500 can return to 514, where the recirculated air can be further ionized to create more free radicals, which can be directed from the second compartment to the first compartment in 516, to react with other microorganisms on the surface of the object(s).

[0067] In 520, after a predetermined period of time, such as 5 minutes, the sanitized object can be removed from the first and / or second compartment. If additional objects need sanitization, method 500 can return to 502, and the entire method can be performed again on the additional objects.

[0068] The system can be useful for storing children's toys, and the first compartment can therefore be configured and used as a box for Petition 870260068441, dated 10 / 07 / 2026, page 21 / 33 18 / 26 toys, for use, for example, in environments where several children use the same toys, such as in a daycare or kindergarten. The system can also be useful for sanitizing other objects that are shared between individuals, including medical instruments in a healthcare setting, shared items in a dormitory, nursing home or office, and pet equipment, among others.

[0069] The embodiments of the present invention are described in more detail with reference to the following study, which is intended to be illustrative and not limiting in nature. Illustrative Study of Test Parameters

[0070] For the study, bacteria were used. Bacteria were chosen because they are more difficult to destroy than viruses, which have more fragile membranes. They are also safer to handle than viruses. The bacteria used are as follows: Human Strains: • Lactobacillus casei HA-108; • Bifidobacterium bifiduk HA-132; • Lactobacillus rhamnosus HA-111; • Lactobacillus rhamnosus HA-114; • Lactobacillus acidophilus R0418; • Bifidobacterium breve R0070; • Bifidobacterium longum spp. children's R0033; • Bifidobacterium longum spp. longum R0175; • Lactobacillus salivarius HA-118; • Lactobacillus reuteri HA-188; • Bifidobacterium longum subsp. Longum R0175; Cepa Comum: • Lactobacillus Plantarum R1012; Cepas Lácteas: • Lactobacillus rhamnosus R0011; Petition 870260068441, dated 10 / 07 / 2026, pág. 22 / 33 19 / 26 • Lactobacillus rhamnosus R1039; • Lactobacillus paracasei HA-196; • Lactobacillus helveticus R0052; • Lactobacillus casei R0215; • Streptococcus salivarius spp. thermophilus R0083; • Lactobacillus delbrueckii spp. bulgaricus R9001; • Lactobacillus helveticus R0052.

[0071] Regarding Streptococcus salivarius spp. thermophilus R0083, Streptococcus thermophilus is a potent probiotic strain with well-researched health benefits. It is widely used in various probiotics, including those for children. This probiotic is frequently found in the colon and has many researched digestive, immune, and other health benefits. S. thermophilus is used in the cultivation of cheeses and yogurts. This probiotic strain also produces lactase, which helps people digest milk more efficiently. Finally, the probiotic Streptococcus thermophilus also produces antibiotic chemicals to prevent infections such as pneumonia and C. difficile, as well as helping to prevent ulcers. Description of Work Protocols

[0072] Bacteria were applied to different test surfaces, each surface being made of different materials, namely: aluminum, Plexiglass, fabric, vinyl, paint, wood, and neoprene (as shown in Figure 6). Phase 1: A sanitization system with a first container (80 L) and a second container (24 L) fluidically coupled was used. A cold plasma ionization emitter was coupled to the second container, a fan was attached between the containers, and a hose was fluidically coupled to both containers to recirculate air between them. A first set of bacteria-contaminated test surfaces was placed in the first container or the lower container (80 L) of the sanitization system, as shown in Figures 7 to 9.

[0073] Free radicals were generated and discharged into the second or upper container (24 L) with a cold plasma ionization emitter for at least 5 minutes. The air / free radicals were directed into the first container. Petition 870260068441, dated 10 / 07 / 2026, page 23 / 33 20 / 26 of 80 L containers containing the contaminated test surfaces. The air from the first 80 L container was recirculated back into the second 24 L container, where more free radicals were generated and discharged into the second 24 L container and directed back into the first 80 L container, etc. Samples from each test surface were collected after each minute that the cold plasma ionization emitter was activated.

[0074] Phase 2: The same sanitization system from Phase 1 was used. A second set of bacteria-contaminated test surfaces was placed in the second or upper container (24 L) of the sanitization system, as shown in Figures 10 and 11. Free radicals were generated and discharged into the second or upper container (24 L) with a cold plasma ionization emitter for at least 5 minutes. Sample swabs from each test surface were collected every minute that the cold plasma ionization emitter was activated.

[0075] Phase 3: A sanitization system with only one first container (80 L) was used. A cold plasma ionization emitter was coupled to and inside the first container, and a fan was attached to the cold plasma ionization emitter to agitate the air inside the first container, as shown in Figures 12 to 16. A third set of bacteria-contaminated test surfaces was placed in the first container (80 L) of the sanitization system. Free radicals were generated and discharged into the first 80 L container via the cold plasma ionization emitter for at least 5 minutes. The concentration of free radicals generated inside the container was maintained within a range of 300,000 to 25,000,000 molecules / cm3. The fan was activated to agitate the air inside the first 80 L container. Sample swabs from each test surface were collected after each minute that the cold plasma ionization emitter was activated. A) Bacteria - MICRO-SOP-202 Method

[0076] A sample was collected with a cotton swab from each test surface, and then the samples were submitted for analysis.

[0077] More specifically, a sample was collected using a sterile swab from the surface suspected of being contaminated with microorganisms, according to the protocol described below. Changing gloves between each collection was important to avoid Petition 870260068441, dated 10 / 07 / 2026, page 24 / 33 21 / 26 contamination of another location with the newly sampled material. Handling methods that promote asepsis were used.

[0078] Sample Collection with a Cotton Swab: 1. Obtain a sterile 1 mL swab of Butterfield Solution to collect and transport the samples; 2. Using gloves, remove the cotton swab from the packaging; 3. Remove the stopper from the medium tube. 4. Carefully pass the cotton swab over the desired area (100 cm2), rolling it slightly back and forth over the sampling area; 5. Insert the cotton swab into the tube, close the cap tightly, and label it appropriately; 6. For quantitative crop reports, the area of ​​swab application needs to be recorded in the chain of custody. Description of the Method

[0079] This standard operating procedure has been used for the identification and quantification of fungi at the genus level and, occasionally, at the species level.

[0080] Results were reported in CFUs per cm2 for air samples collected on agar plates, e.g., Andersen plates or RCS Biotest strips; CFUs (colony forming units) per swab, unless an area where the swab passed was specified; CFUs per gram for bulk samples; CFUs per sample for contact plates and glove tips; and CFUs per mL for liquid / water samples.

[0081] Applicable Matrices: Air, cotton swab, water, volume (liquids and solids), contact plates (RODAC) and glove tips. Test method

[0082] Suspensions and dilutions were prepared from swabs, volume and liquid / water samples, and then incubated on an agar plate.

[0083] Table 1: Usual growth conditions Agar Incubation temperature Incubation time Bacteria Tryptone Soy Agar (TSA) 37 °C 24 to 72 hours Petition 870260068441, dated 10 / 07 / 2026, page 25 / 33 22 / 26

[0084] Sample Collection, Preservation, Shipping and Storage Samples were collected in sterile sampling containers and shipped in a cooler with ice. Customers are advised to ship samples to the laboratory within 24 hours (express delivery), or samples must be kept refrigerated until shipment. Samples are stored for a maximum of 2 days before shipment to the laboratory or preparation. After receipt of samples at the laboratory, they can be kept at room temperature for a maximum of 6 hours before preparation. If it is not possible to prepare the samples within 6 hours, they are refrigerated.

[0085] Limit of Detection: Also reported as Analytical Sensitivity or Limit of Detection (LOD), the limit of detection is equal to 1 colony-forming unit (CFU) per plated dilution. As a general rule, 3 dilutions are performed on a client sample (100X, 1,000X, and 10,000X), unless the sample warrants otherwise. For USP samples <797> Only 10X and 100X dilutions need to be plated. The lowest plated dilution is used to calculate the reported LOD. For example, if the sample was diluted 10, 100, and 1000 times, the limit of detection would be the lowest dilution in this series. In this case, 10, therefore, LOD = 10 CFU / sample. For customers submitting agar plates, the LOD is 1 CFU per plate.

[0086] Calibration and Standardization: All incubators, water baths and analytical balances have been checked and maintained in accordance with ISO standards.

[0087] Sample Analysis - Interpretation and Calculations: After incubation, every bacterial colony on each agar dilution plate was counted. All growth was identified using dilution supports (“tease mounts”) or cellophane tape supports with dye or oil for microscopic identification, using appropriate literature and identification manuals.

[0088] The final results reported were dependent on the dilution factor used. Final results were read on day 5. However, preliminary counts were performed on day 3. If no bacterial colonies were found on day 7, the value would be “less than” the lowest LOD. If plates could not be counted at the end of the incubation period, they would be stored for later enumeration, refrigerated in a sealable container for a maximum of one week. Petition 870260068441, dated 10 / 07 / 2026, page 26 / 33 23 / 26

[0089] Quality Control: All QC data were maintained and made available for easy consultation and inspection. All analysts underwent documented training and completed the fungal culture training checklist. Each laboratory adhered to the quality control and quality assurance procedures described in the laboratory's QMS Manual. Quality control of the agar medium was performed by the manufacturer and internally. The manufacturer's quality control batch was recorded, as well as internal sterility and positive / negative reaction controls. The laboratory performed quality control for the internally produced lactophenol blue or lactofuchsin dye, by batch. Quality control organisms were routinely cultured and used as positive and negative controls and as identification aids. The microscopes used in this analysis were maintained according to MICRO-SOP-05 Microscope Use.

[0090] Blank Samples: A blank sample was generated each day for swab or bulk samples; as per Culture Blank Log Inter, 5% inter-reanalysis was performed on customer samples; as per MICRO-SOP-16 Intra: 5% intra-reanalysis was performed; as per MICRO-SOP-16. Standards: All standard fungal cultures were maintained as per MICRO-SOP-202-3.

[0091] Reference values ​​for bacterial count: • From 0,000 CFU / 100 cm² to 10,000 CFU / 100 cm² = very low • From 10,000 CFU / 100 cm² to 20,000 CFU / 100 cm² = low • From 20,000 CFU / 100 cm² to 50,000 CFU / 100 cm² = medium • From 50,000 CFU / 100 cm² to 100,000 CFU / 100 cm² = high • From 100,000 CFU / 100 cm² to 200,000 CFU / 100 cm² = very high • From 200,000 CFU / 100 cm² to 500,000 CFU / 100 cm² = very, very high • From 500,000 CFU / 100 cm² 2,000,000 CFU / 100 cm2 = extremely high Patterns and Analysis

[0092] Samples for bacteria were analyzed by the microbiology laboratory in Quebec City, QC, Canada. This laboratory brings together more than 10 specialized laboratories in Quebec and Ontario.

[0093] All samples collected were analyzed in laboratories and approved by microbiologists specializing in microbiology. All methods of Petition 870260068441, dated 10 / 07 / 2026, page 27 / 33 24 / 26 sampling was in compliance with the standards and regulations of the Quebec Institute for Health and Safety Research (IRSST) and the Commission on Standards, Equity, Health and Safety at Work (CNESST). Laboratory sample management is carried out by biotechnologists, microbiologists and chemists, each specializing in cutting-edge sectors of their respective disciplines.

[0094] All laboratory analytical procedures have been recognized or accredited in accordance with ISO / IEC 17025. The various laboratory analysis methodologies comply with international standards recognized by IOSH, ACGIH, OSHA, ASTM, AOAC, FDA, BAM, CTFA, USP, APHA, ASM and EPA, the US Environmental Protection Agency.

[0095] The laboratory's internal Quality Assurance and Quality Control (QA / QC) programs also comply with ISO 17025. The laboratory's analytical work is also consistent with CMHC Standards and Health Canada.

[0096] Table 2: Results Sample No. Name Phase 1 80 liters CFU / m3 Phase 2 24 liters CFU / m3 Phase 1 80 liters CFU / m3 1 Aluminum - Contaminated > 300,000 259,000 > 300,000 2 Aluminum 1 min. EXP 137,500 2,364 8,900 3 Aluminum 2 min. EXP 72,000 437 6,900 4 Aluminum 3 min. EXP 15,900 230 6,150 5 Aluminum 4 min. EXP 16,900 510 4,050 6 Aluminum 5 min. EXP 7,200 126 1,450 7 Plexiglass - Contaminated > 300,000 > 300,000 > 300,000 8 Plexiglass 1 min. 16,600 3,350 39,500 9 Plexiglass 2 min. 6,550 645 34,500 10 Plexiglass 3 min. 4,600 575 14,850 11 Plexiglass 4 min. 1,100 415 4,650 12 Plexiglass 5 min. 1,930 171 2,110 13 Tissue - Contaminated > 300,000 > 300,000 > 300,000 14 Tissue 1 min. 72 < 10 50 15 Fabric 2 min. 32 < 10 42 16 Fabric 3 min. 41 < 10 90 17 Fabric 4 min. 59 < 10 23 18 Fabric 5 min. 50 < 10 54 19 Vini - Contaminated > 300,000 > 300,000 > 300,000 20 Vinyl 1 min. 63,500 1,705 9,400 21 Vinyl 2 min. 23,500 1,600 2,540 22 Vinyl 3 min. 6,000 565 1,355 23 Vinyl 4 min. 4,050 510 2,305 24 Vinyl 5 min. 1.060 405 585 25 Painting - Contaminated 269,000 > 300,000 > 300,000 26 Painting 1 min. 7,200 7,800 10,100. Petition 870260068441, dated 10 / 07 / 2026, page 28 / 33 25 / 26 27 Painting 2 min. 3,850 1,685 7,750 28 Painting 3 min. 3,150 675 1,520 29 Painting 4 min. 1,560 940 2,800 30 Painting 5 min. 625 550 1,525 31 Wood - Contaminated 278,000 > 300,000 > 300,000 32 Wood 1 min. 1,360 475 930 33 Wood 2 min. 605 275 345 34 Wood 3 min. 135 290 650 35 Wood 4 min. 261 176 216 36 Wood 5 min. 390 68 234 37 Neoprene - Contaminated 254,500 269,000 251,000 38 Neoprene 1 min. 8,600 27,800 42,500 39 Neoprene 2 min. 3,750 5,800 24,727 40 Neoprene 3 min. 2,600 5,700 10,900 41 Neoprene 4 min. 1,400 5,450 10,600 42 Neoprene 5 min. 1,210 4,250 5,850

[0097] Based on the results above, it was found that the present system, method and use of surface sanitization of objects with free radicals were efficient in eliminating bacteria on the given test surfaces and effective in destroying surface contaminants. Each configuration achieved a reduction of more than 90% of bacteria on all surfaces after 5 minutes of system operation.

[0098] As noted above, the concentration of free radicals generated within the container was maintained between 300,000 and 25,000,000 molecules / cm3. The concentration of free radicals proved effective in reducing bacteria on all surfaces within the mentioned range. Higher concentration levels would increase effectiveness, but with diminishing returns. Lower concentration levels would also work, but would likely require longer dwell times to supplement the lower dosage.

[0099] Although this disclosure describes methods and processes with operations (e.g., steps) in a particular order, one or more operations of the methods and processes may be omitted or altered as appropriate. One or more operations may occur in a different order than that in which they are described, as appropriate.

[0100] All values ​​and subranges within the disclosed ranges are also disclosed. Furthermore, although the systems, devices and processes disclosed and shown in the present invention may comprise a specific number of Petition 870260068441, dated 10 / 07 / 2026, page 29 / 33 26 / 26 elements / components, the systems, devices and assemblies can be modified to include more or fewer of these elements / components. For example, although any one of the disclosed elements / components may be referred to as singular, the embodiments disclosed in the present invention can be modified to include a plurality of such elements / components. The subject matter described in the present invention is intended to cover and encompass all suitable technological changes.

[0101] This disclosure may be incorporated into other specific forms without departing from the subject matter of the claims. The exemplary embodiments described should be considered, in all respects, merely illustrative and not restrictive. Selected features from one or more of the embodiments described above may be combined to create alternative embodiments not explicitly described, the features suitable for such combinations being understood within the scope of this disclosure. Petition 870260068441, dated 10 / 07 / 2026, pages 30 / 33

Claims

1 / 4 CLAIMS 1. System for sanitizing the surface of an object, CHARACTERIZED by comprising: a first compartment having a first opening dimensioned to receive the object through it and having a first lid dimensioned to cover the first opening, the first lid being configured to be resealable over the first opening of the first compartment, and an air ionizer coupled to the first compartment to generate free radicals for discharge into the first compartment, wherein the reaction of the free radicals with microorganisms on the surface of the object inactivates the microorganisms.

2. System according to claim 1, CHARACTERIZED in that the air ionizer is a cold plasma emitter.

3. System according to any one of claims 1 to 2, CHARACTERIZED in that the free radicals comprise hydroxyl radicals.

4. System according to any one of claims 1 to 3, CHARACTERIZED in that it further comprises a fan coupled to the first compartment, the fan being configured to agitate the air within the first compartment.

5. System according to any one of claims 1 to 4, CHARACTERIZED in that the air ionizer is configured to generate free radicals at a rate of at least 400x106 molecules / cm3.

6. System according to any one of claims 1 to 5, CHARACTERIZED in that the first compartment has a volume of 150 L or less.

7. System according to any one of claims 1 to 6, CHARACTERIZED in that the air ionizer is positioned inside the first compartment to generate free radicals from the air inside the first compartment.

8. System according to any one of claims 4 to 6, CHARACTERIZED in that it further comprises a second compartment in fluid communication with the first compartment, wherein the fan is positioned between the first and second compartments to direct air from one compartment to the other.

9. System according to claim 8, CHARACTERIZED in that the air ionizer is attached to the second compartment to generate free radicals therein, the fan being positioned between the first and second compartments to discharge the free radicals from the second compartment into the first compartment.

10. System according to claim 8 or 9, CHARACTERIZED in that it further comprises an air channel, one end of the air channel being fluidly coupled to the first compartment and the opposite end of the air channel being fluidly coupled to the second compartment.

11. System according to any one of claims 8 to 10, CHARACTERIZED in that it further comprises a second opening positioned in the second compartment and a second cover sized to cover the second opening, the second cover being configured to be resealable over the second opening of the second compartment.

12. System according to any one of claims 1 to 11, CHARACTERIZED in that the first compartment is a toy box for storing children's toys.

13. Method for sanitizing the surface of an object, CHARACTERIZED by comprising: placing the object in a first compartment through a first opening sized to receive the object through it; sealing the first opening of the first compartment with a resealable lid, and generating free radicals with an air ionizer and discharging the free radicals into the first compartment, where the reaction of the free radicals with microorganisms on the surface of the object inactivates the microorganisms.

14. Method according to claim 13, CHARACTERIZED in that the air ionizer is a cold plasma emitter. Petition 870250071833, dated 08 / 14 / 2025, pp. 88 / 91 3 / 4 15. Method according to any one of claims 13 to 14, CHARACTERIZED in that the free radicals comprise hydroxyl radicals.

16. Method according to any one of claims 13 to 15, CHARACTERIZED in that it further comprises maintaining the concentration of free radicals in the first compartment within 300,000 to 25,000,000 molecules / cm3.

17. Method according to claim 16, CHARACTERIZED in that the concentration of free radicals is maintained within the first compartment for at least 5 minutes.

18. Method according to any one of claims 13 to 17, CHARACTERIZED in that it further comprises agitating the air within the first compartment with a fan.

19. Method according to any one of claims 13 to 18, CHARACTERIZED in that the first compartment has a volume of 80 L or less.

20. Method according to claim 18, CHARACTERIZED in that the generation and discharge of free radicals comprise: generating the free radicals in a second compartment that is in fluid communication with the first compartment, and directing the free radicals from the second compartment to the first compartment with the fan.

21. Method according to claim 20, CHARACTERIZED in that it further comprises the circulation of air from the first compartment to the second compartment.

22. Use of free radicals to sanitize the surface of an object, CHARACTERIZED by comprising: generating and discharging free radicals in a sealed compartment containing the object, the compartment having a resealable lid, wherein the reaction of free radicals with microorganisms on the surface of the object inactivates the microorganisms.

23. Use according to claim 22, CHARACTERIZED by the fact that it is for sanitizing health instruments. Petition 870250071833, dated 08 / 14 / 2025, pp. 89 / 91 4 / 4 24. Use according to claim 22, CHARACTERIZED by the fact that it is for sanitizing shared objects used in shared spaces.

25. Use according to claim 22, CHARACTERIZED by the fact that it is for sanitizing pet equipment. Petition 870250071833, dated 08 / 14 / 2025, pp. 90 / 91

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