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Cultivation method for highly effective plant tissue differentiation and regeneration

A technology for inducing plants and culturing methods, applied in the field of plant tissue culture, and can solve the problems of low differentiation and regeneration rate and slow regeneration speed.

Inactive Publication Date: 2008-08-20
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although tissue culture technology has been used to successfully establish in vitro regeneration systems in many plant species, due to species differences and limitations in culture conditions, the differentiation and regeneration rates of many plants are not high and the regeneration speed is slow, which cannot meet the needs of actual production and production. Therefore, it is very important to improve the current tissue culture technology, especially for the induction and differentiation of woody plants in more plants, so as to achieve a higher rate of differentiation and regeneration and regeneration speed.

Method used

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  • Cultivation method for highly effective plant tissue differentiation and regeneration
  • Cultivation method for highly effective plant tissue differentiation and regeneration
  • Cultivation method for highly effective plant tissue differentiation and regeneration

Examples

Experimental program
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Embodiment 1

[0029] Embodiment 1. The tissue culture of the blade regeneration plant of Seoul loose-leaf lettuce

[0030] 1. Soak Seoul loose-leaf lettuce seeds (purchased from Beijing Jinxiu Dadi Company) with mercury chloride for 5 minutes, put them on MS solid medium, cultivate them at 24°C under light for 12h / d, and subculture on MS solid medium after germination to cultivate.

[0031] 2. Get young and tender lettuce true leaves, leaf diameter 2-3cm, cut into 1cm * 1cm square (explant), place the callus that betaine aldehyde (purchased from U.S. DNA Polymerase Technology company) concentration is 5mM on the back side Induction and differentiation solid medium (callus induction and differentiation medium: MS basic medium + IAA (Sigma) 1.0 mg / L + 6-BA (Sigma) 0.2 mg / L, pH 5.8).

[0032] 3. Cultivate at 24°C and light 12h / d.

[0033] 4. After 11 days, calluses began to grow around the explants, and in about 20 days, they began to differentiate into bud points or sprouts, and in about 30...

Embodiment 2

[0039] Example 2. Effects of Different Concentrations of Betaine Aldehyde on Inducing Plant Tissue Differentiation and Regeneration

[0040]The betaine aldehyde concentration added in callus induction and differentiation medium is 4,6,10mM, carries out the tissue culture of the blade regeneration plant of Seoul loose-leaf lettuce in the same manner as embodiment 1 and comparative example 1, all can be different Promote the differentiation regeneration of lettuce to a certain extent, differentiation rate and regeneration rate are all higher than control example, but lower than embodiment 1 (see Figure 6 , taking 10mM as an example, the rest are similar), therefore, 5mM is the optimal concentration that betaine aldehyde promotes the differentiation and regeneration of lettuce leaves.

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Abstract

The present invention relates to tissue culture method with high efficiency induction of plant tissue regeneration, and features that during plant callus induction and differentiation, betaine aldehyde is added into the culture medium to raise the differentiation and regeneration rate of callus bud, shorten the regeneration bud obtaining period and raise the growth speed of the regenerated bud effectively. The said method can raise the regeneration rate of callus by over 1 time, move up the germination time by 5 days and shorten the culture period from the inoculation of explant to transformation to the rooting culture medium by 10-15 days. The present invention also relates to the callus or contagious bud obtained by means of the said method as well as plant obtained through differentiation of the callus or contagious bud.

Description

Technical field: [0001] The present invention relates to plant tissue culture method. Background technique: [0002] Tissue culture technology refers to the cultivation of plant tissue using artificial medium under sterile conditions. Tissue culture technology can not only provide ideal recipient materials for genetic engineering, but also provide new means for routine plant improvement procedures, creating more, faster and better new varieties, and has a wide range of uses in agriculture; It is an indispensable part of transgenic technology; it can rapidly propagate important and valuable plant varieties; it has important practical significance in crop detoxification, etc. [0003] Conventional tissue culture is to add various hormones on the basis of the basic medium containing the nutrients necessary for plant growth, use the totipotency of plant cells to induce their dedifferentiation to form callus, then differentiate into adventitious buds, and finally induce adventit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 胡赞民李朔胡军陈宇红尹维波
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI