Method for producing angiotonin converzyme inhibiting peptide by whey proteinase method
A technology of angiotensin and whey protease, which is applied to the preparation method of peptides, chemical instruments and methods, peptides, etc., to achieve high safety effects
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Embodiment 1
[0022] Take 6g of whey protein isolate (WPI9000), dissolve it in 100ml of water, then heat at 90°C for 10min, cool down to 37°C and adjust the pH to 8.0, then add 0.16g of trypsin, hydrolyze in a constant temperature water bath shaker at 37°C for 12h, hydrolyze During the process, 0.1N NaOH was continuously added to keep the pH at 8.0 (the difference between the upper and lower sides was not more than 0.1). After hydrolysis, the enzyme was inactivated by heating at 85°C for 20 minutes, then cooled and then freeze-dried. Part of the dry matter was taken for in vitro detection of ACE inhibitory activity. The ACE inhibitory activity of the hydrolyzate was 71.51%±2.02%
Embodiment 2
[0024] Take 6g of whey protein isolate (WPI9000), dissolve it in 100ml of water, then heat at 90°C for 10min, cool down to 50°C to adjust the pH to 7.0, then add 0.16g of neutral protease, and hydrolyze it in a constant temperature water bath shaker at 50°C for 12h. During the hydrolysis process, 0.1N NaOH was continuously added to keep the pH at 7.0 (the difference between the upper and lower sides was not more than 0.1). After the hydrolysis was completed, the enzyme was inactivated by heating at 85°C for 20 minutes, then cooled and then freeze-dried. Part of the dried product was taken for in vitro detection of ACE inhibitory activity. The ACE inhibitory activity of the hydrolyzate was 82.74%±0.93%.
Embodiment 3
[0026] Take 6g of whey protein isolate (WPI9000), dissolve it in 100ml of water, then heat at 90°C for 10min, cool down to 60°C to adjust the pH to 8.5, then add 0.16g of alkaline protease, and hydrolyze it in a constant temperature water bath shaker at 60°C for 12h. During the hydrolysis process, 0.1N NaOH was continuously added to keep the pH at 8.5 (the difference between the upper and lower sides was not more than 0.1). After the hydrolysis, the enzyme was inactivated by heating at 85°C for 20 minutes, and then cooled and then freeze-dried. Part of the dried product was taken for in vitro detection of ACE inhibitory activity. The ACE inhibitory activity of the hydrolyzate was 90.68%±2.83%.
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