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Polybutylcyanoacrylate nano-granule with resolubity, application and use thereof

A technology of polycyanoacrylate and cyanoacrylate, which is applied in the field of resolvable polycyanoacrylate nanoparticles, its preparation and pharmaceutical application, can solve the problems of inconvenient use and storage, adhesion, mildew, etc., and achieve The ability to carry nucleic acid does not decrease, the Zeta potential is high, and the stability is good

Inactive Publication Date: 2009-02-11
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the product that adopts this method to make, its solution is not suitable for long-term storage, room temperature is placed prone to coagulation, mildew, generally adopts low-temperature storage, use and preservation are all inconvenient; Sometimes it cannot be redissolved in water and other media, often occurs adhesion and other phenomena, loses the properties of the original nano-sized particles, and cannot be used as a nucleic acid carrier

Method used

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  • Polybutylcyanoacrylate nano-granule with resolubity, application and use thereof
  • Polybutylcyanoacrylate nano-granule with resolubity, application and use thereof
  • Polybutylcyanoacrylate nano-granule with resolubity, application and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Weigh 1.2 parts of DEAE-Dextran and 3.0 parts of Dextran-70, dissolve them in 250 parts of hydrochloric acid solution with a pH of 2.0, add 1.6 parts of methyl cyanoacrylate monomer dropwise to the above mixed solution under stirring conditions, After the dropwise addition, continue to stir for 6 hours, then add 25 parts of glucose and 10 parts of mannitol, and stir for 15 minutes to obtain a suspension of polymethylcyanoacrylate nanoparticles; pre-freeze at a low temperature of -40°C for about 5 hours, After the pre-freezing is completed, take it out, quickly put it into a freeze dryer for freeze-drying, until the water is completely removed, and then obtain the freeze-dried product of polymethyl cyanoacrylate nanoparticles, which is white and loose.

[0021] Reconstitution method: when in use, take a certain amount of polycyanoacrylate methyl ester nano-particle freeze-dried powder prepared above, add an appropriate amount of ultrapure water to re-dissolve, and form a ...

Embodiment 2

[0025] Weigh 1.2 parts of DEAE-Dextran and 2.4 parts of Dextran-70, dissolve them in 250 parts of hydrochloric acid solution with a pH of 2.5, add 2.3 parts of methyl cyanoacrylate monomer dropwise to the above mixed solution under stirring conditions, After the dropwise addition, continue to stir for 6 hours, then add 25 parts of glucose and 10 parts of mannitol, and stir for 15 minutes to obtain a suspension of polymethylcyanoacrylate nanoparticles; pre-freeze at a low temperature of -40°C for about 5 hours, After the pre-freezing is completed, take it out, quickly put it into a freeze dryer for freeze-drying, until the water is completely removed, and then obtain the freeze-dried product of freeze-reconstituted polymethyl cyanoacrylate nanoparticles, which is white and loose in shape.

[0026] The redissolving method is the same as in Example 1.

[0027] Table 2 Main indicators before and after reconstitution of polymethyl cyanoacrylate nanoparticles

[0028] sam...

Embodiment 3

[0030] Weigh 1.5 parts of DEAE-Dextran and 1.5 parts of Dextran-70, dissolve them in 100 parts of hydrochloric acid solution with a pH of 2.2, add 2.6 parts of ethyl cyanoacrylate monomer dropwise to the above mixed solution under stirring conditions, drop After the addition is complete, continue to stir for 4 hours, then add 20 parts of glucose and 15 parts of mannitol, and stir for 20 minutes to obtain a suspension of polyethylcyanoacrylate nanoparticles; pre-freeze at a low temperature of -40°C for about 5 hours to complete Take it out after pre-freezing, and quickly put it into a freeze dryer for freeze-drying until the water is completely removed, and then you can get the freeze-dried product of polyethylcyanoacrylate nanoparticles, which is white and loose in shape.

[0031] The redissolving method is the same as in Example 1.

[0032] Table 3 Main indicators before and after reconstitution of polyethylcyanoacrylate nanoparticles

[0033] sample Particle siz...

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Abstract

The invention relates to a double soluble polycyanoimide acrylate nanometer particle, which is formed by polycyanoimide acrylate, DEAE-Dextran, Dextran-70, amylaceum and mannite, while the mass percentages of said components are polycyanoimide acrylate at 2.0-16.3%, DEAE-Dextran at 1.9-8.8%, Dextran-70 at 3.3-19.0%, amylaceum at 30.3-79.6% and mannite at 8.3-52.8%. And the invention also provides a relative preparation, which uses polycyanoimide acrylate as raw material, and uses the polymerization emulsion method to prepare the polycyanoimide acrylate nanometer particle, with added protective agent, to cool and dry to obtain the product. The invention can be double dissolved completely, as the carrier of nucleinic acid, to avoid degrade DNA enzyme, improve the cell extract rate, and target cancer cell.

Description

Technical field [0001] The method belongs to the technical field of homopolymer or copolymer compositions with one or more unsaturated aliphatic compounds, and specifically relates to a polycyanoacrylate nanoparticle and its preparation technology. Background technique [0002] Gene therapy is currently a research hotspot in the field of pharmacy and medicine. Nucleic acid therapy is one of the important fields. However, nucleic acid is easily degraded by DNase after entering the body, and the cellular uptake rate is low, making it difficult to achieve the desired therapeutic effect. Polycyanoacrylate nanoparticles are ideal carriers of nucleic acids, which have the characteristics of preventing DNase degradation, increasing cell uptake rate, and inhibiting tumor cells. The paper "Preparation of n-butyl cyanoacrylate nanoparticle gene carrier and in vitro study of related properties" written by Zhang Yangde et al., published in the first issue of volume 14 of "Chinese Journa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/32A61K48/00C08F120/34C08F2/44A61P35/00
Inventor 张振中周天洋
Owner ZHENGZHOU UNIV