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A preparation and an application of epiderm or hair follicle stem cells from sources of human early embryo

A technology for epidermal stem cells and hair follicle stem cells, applied in the field of human embryo-derived epidermal stem cells, can solve the problems of harsh culture conditions and slow proliferation of epidermal stem cells

Inactive Publication Date: 2009-03-04
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, adult-derived epidermal stem cells proliferate slowly, and the culture conditions are harsh

Method used

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  • A preparation and an application of epiderm or hair follicle stem cells from sources of human early embryo
  • A preparation and an application of epiderm or hair follicle stem cells from sources of human early embryo
  • A preparation and an application of epiderm or hair follicle stem cells from sources of human early embryo

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Experimental program
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Embodiment 1

[0025] (1) Preparation of total RNA from human epidermal cells:

[0026] Epidermal cells were obtained from adult human skin through primary culture (Yang Ling, Liu Houqi, etc. Localization, isolation and culture of epidermal stem cells from adult human skin. Journal of Second Military Medical University, 2004, 25(8): 815-817.). The total RNA was obtained by extracting the total RNA by the conventional guanidine isothiocyanate method with the total RNA extraction kit of Shanghai Huashun Bioengineering Co., Ltd.

[0027] (2) Synthesis of primers and construction of pCDNA3.0-EGF eukaryotic expression vector:

[0028] The hEGF gene was transferred to the eukaryotic vector pCDNA3.0. Obtain the cDNA fragment containing the EGF gene according to the literature and recombine it into the eukaryotic expression vector pCDNA3.0 (refer to Chen Tingsu et al., Synthesis and identification of human epidermal growth factor (hEGF) gene and construction of plant expression plasmid, Journal of ...

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Abstract

This invention provides a method for accelerating the proliferation of human mesenchymal stem cells, which comprises the steps of: transfecting recombinant expression carrier containing EGF to human mesenchymal stem cells, and stimulating with a certain concentration of calcium ions during the transfection process to divide into typical stem cells. Under nude mouse subcutaneous microenvironment induction, the stem cells are further divided into skin tissue stem cells, thus directly taking part in the proliferation of nude mouse hair. This invention can obtain rapidly proliferated human mesenchymal stem cells, thus having a potential application in clinical skin wound treatment.

Description

technical field [0001] The invention relates to the technical field of stem cells and genetic engineering, in particular to a method and application of human embryo-derived epidermal (hair follicle) stem cells capable of rapid proliferation. Background technique [0002] The skin is the largest organ of the human body, and it is easily damaged because it is located on the surface of the body. Such as burns, scalds, contusions, trauma, etc. are clinically very common. The coverage of skin wounds and the regeneration of epidermal cells are the key to the treatment of skin injuries. The root of trauma treatment lies in wound healing. At present, autologous skin transplantation, autologous allogeneic hybrid transplantation, or autologous allogeneic skin replantation are still used for repairing large-area deep wounds. These methods have long treatment period, many complications, high treatment cost, poor healing quality, and cause New wounds and other disadvantages. Regenera...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/85C12N5/10A61P17/02A61K35/36
Inventor 刘厚奇仵敏娟杨玲
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY