Method of separating and purifying destruxins of Metarrhizium anisopliae

Abstract

The invention discloses a separating and purifying method of Metarrhizium anisopliae, which comprises the following steps: (1) adsorbing fungal ferment liquid to remove partial impurity; removing adsorbent through centrifuging; obtaining supernatant layer of ferment liquid; (2) extracting supernatant layer under ultrasound; separating organic phase and water phase through separatory funnel; reserving organic phase to condense into rough extract of Metarrhizium anisopliae; (3) decompressing rough extract of Metarrhizium anisopliae; condensing and drying fraction; dissolving and filtering; (4) making spectrum; separating and purifying the rough product in the high-effective liquid phased chromatograph to produce high-purity product.

Description

Method for Separating and Purifying Metarhizin technical field The invention relates to a method for separation and purification, in particular to a method for separation and purification of metarhizine. technical background Metarhizium (destruxin) is a mycotoxin, is a class of cyclic decarboxypeptide compounds, mainly produced by Metarhizium, other fungi such as Aschersonia, Alternaria, single terminal Trichothecium and Ophiosphaerella can also form different types of metarhizine. Metarhizine has biological activities such as insecticidal, antiviral and anticancer, and has high economic value. Since Kodaira isolated metarhizine A and B for the first time in 1961, 35 metarhizines have been analyzed and identified. From Kodaira (1961), Susuki (1970) to Pail et al. (1981) all adopt the traditional classical method to isolate metarhizine. The first step of the method is extraction, the fermentation broth of Metarhizium anisopliae is centrifuged or filtered to remove slag, ...

AI Technical Summary

Problems solved by technology

So far, although a variety of metarhizines have been identified, most of them focus on structural analysis and determination of physical and chemical properties. As for the separation and preparation methods, they are still limited to complicated processes, and the entire process requires the use of a large amount of highly toxic solvents. , lasts for a long time and is very expensive to prepare

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