Double phospho acid compound of 99mT.C. mark and preparation method
A technology of bisphosphonic acid and complex, which is applied in the fields of radiopharmaceuticals and nuclear medicine to achieve good effects
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[0026] Technetium-99m-labeled 2-(1-methylimidazol-2-yl)-1-hydroxyethane-1,1-bisphosphonic acid complex ( 99m Tc-HMIBP) Preparation and Performance Determination
[0027] 1) 99m Preparation of Tc-HMIBP
[0028] In a 10ml vial, add 3-5 mg of 2-(1-methylimidazol-2-yl)-1-hydroxyethane-1,1-bisphosphonic acid, SnCl 2 2H 2 O hydrochloric acid solution 0.05 ~ 0.25mL (the concentration of hydrochloric acid is 0.1mol L -1 , containing 1.0g·L -1 SnCl 2 2H 2 O), after shaking well, use phosphate buffer solution (pH4.3) to adjust the pH to 4~6, add freshly rinsed pertechnetium-99m acid ( 99m TCO 4 - ) solution of 18.5 to 185 megabeq (MBq), fully mixed, placed at room temperature for 8 to 15 minutes, and the labeling is completed, and the technetium-99m labeled 2-(1-methylimidazol-2-yl )-1-hydroxyethane-1,1-bisphosphonic acid complex ( 99m Tc-HMIBP).
[0029] 2) 99m Determination of Tc-HMIBP labeling rate
[0030] The labeling rate of technetium-99m-labeled imidazole bisphosph...
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