Gas emulsion type Ultrasound Contrast Agent microspheres and the preparing method thereof

An ultrasonic contrast agent and air emulsion technology, applied in echo/ultrasonic imaging agents, etc., can solve the problems of organic solvent residue, reduced elasticity of microspheres, short retention time, etc. Effect

Inactive Publication Date: 2007-10-31
NAN FANG HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Albumin microspheres have been used earlier, and existing products have been developed and marketed. The outer shell of albumin microspheres is a heat-denatured protein, which reduces the elasticity (ie, compressibility) of the microspheres, so the retention time in the body is shorter. To some extent, its ap

Method used

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  • Gas emulsion type Ultrasound Contrast Agent microspheres and the preparing method thereof
  • Gas emulsion type Ultrasound Contrast Agent microspheres and the preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0025] Example 1: The effect of poloxamer on the formation of microspheres

[0026] Weigh lecithin at 0.1% by weight, weigh PEG4000-distearoylphosphatidylethanolamine at 0.005% by weight, and mix with 0%, 0.015%, 0.025%, and 0.035% by weight of poloxamer, respectively , Add it to 5% glucose solution and mix thoroughly at 50-60℃, pass in perfluoropropane gas, extend the homogenizer dispersing knife into the liquid surface for high-speed shearing and dispersion, and prepare milky white contrast medium micro For the ball suspension, the concentration and particle size of the microspheres were measured with a Coulter particle size analyzer. The results are shown in Table 1.

[0027] Poloxamer concentration

[0028] The results show that the addition of an appropriate amount of poloxamer can help the formation of aeroemulsion microspheres and generate a high-concentration microsphere suspension.

Example Embodiment

[0029] Example 2: The effect of equipment rotation speed on the yield and particle size of microspheres

[0030] Weigh lecithin at 0.1% by weight, weigh PEG4000-distearoylphosphatidylethanolamine at 0.005% by weight, and poloxamer at 0.025% by weight. Add to 5% glucose solution and mix thoroughly at 50-60°C. Evenly, pass in perfluoropropane gas, extend the homogenizer dispersing blade into the liquid surface, and perform high-speed shear dispersion on the solution at different speeds. The concentration and particle size data of the prepared microspheres are shown in Table 2.

[0031] Speed (r / min)

[0032] It can be seen from Table 2 that as the rotation speed of the dispersing cutter head increases, the concentration of the prepared microspheres increases and the average particle size decreases.

Example Embodiment

[0033] Example 3: Pressure resistance test of microspheres

[0034] Weigh lecithin at 0.1% by weight, weigh PEG4000-distearoylphosphatidylethanolamine at 0.005% by weight, and poloxamer at 0.025% by weight, and prepare it at 24000r / min according to the method described in Example 1. The contrast agent microsphere suspension is divided into ampoules, and the pressure of 100mmHg, 200mmHg, and 300mmHg is applied to the suspension for 2 minutes with a pressurizing device, and then the concentration and particle size of the microspheres are measured with a Coulter. The data comparison before and after is shown in Table 3.

[0035] Withstand pressure (mmHg)

[0036] It can be seen from Table 3 that the ultrasound contrast agent microspheres of the present invention have good pressure resistance, can fully tolerate the effect of human arterial pressure after intravenous injection, and achieve the effect of ultrasound imaging contrast enhancement.

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Abstract

The invention discloses a new type gas emulsion supersonic contrast agent and preparing method, which is characterized by the following: choosing phosphatide, carbowax derivant and Bolusham as raw material; choosing the coating gas as water-insoluble gas with bioavailability; adding the raw material into equal liquid penetration; dispersing completely; proceeding supersonic vibrate or mechanical cutting; getting the product. This invention possesses low cost, simple preparation and good stability.

Description

field of invention [0001] The invention relates to an ultrasonic contrast agent, specifically a gas emulsion type ultrasonic contrast agent microsphere formed by wrapping fluorocarbon insoluble gas with phospholipids, polyethylene glycol derivatives and poloxamers as spherical wall components , and relates to its preparation method. Background of the invention [0002] Compared with other clinical medical diagnostic techniques, ultrasound has the advantages of no radiation and low price. During ultrasonic imaging examination, the ultrasonic instrument emits ultrasonic waves, and then receives the acoustic signal reflected by the organ tissue structure for data conversion to form an image of the area to be inspected. [0003] The reflection of sound waves at interfaces of different acoustic densities (such as liquid-gas) is extremely efficient. Therefore, the gas-containing microspheres made of suitable ball wall materials wrapped with gas can be used as ultrasound contrast...

Claims

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Application Information

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IPC IPC(8): A61K49/22
Inventor 侯连兵刘伊丽杨莉查道刚王新亚宾建平杨西晓王月刚
Owner NAN FANG HOSPITAL
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