Zymolysis process for preparing high-immunity regulation activity wolfberry polysaccharides using wolfberry wine residues

A technology of Lycium barbarum polysaccharide and Lycium barbarum wine is applied in the directions of food preparation, plant raw materials, allergic diseases, etc., which can solve the problem of low immunomodulatory activity and achieve the effect of improving immunomodulatory activity.

Inactive Publication Date: 2007-11-14
HUBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, the primary polysaccharides of Lycium barbarum extracted by general wat

Method used

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  • Zymolysis process for preparing high-immunity regulation activity wolfberry polysaccharides using wolfberry wine residues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Dry 200 grams of Lycium barbarum wine residue, grind it into primary powder, add 1.4 L of water, extract twice at 80° C. for 3 hours each time, and combine all the filtrates. Concentrate under reduced pressure at 70°C to 1 / 10 of the original volume. Add 95% ethanol to the concentrated solution to make the alcohol content reach 80%, and let it stand for 18 hours to precipitate out. The alcohol precipitate was vacuum-dried at 50°C, the obtained Lycium barbarum polysaccharide was dissolved in 250mL of water, 50mL of 0.004% saccharification enzyme solution was added, and hydrolyzed at 40°C for 30min. The hydrolyzate was boiled for 10 minutes to inactivate the enzyme. Add 95% ethanol to make the alcohol content reach 80%, let it stand still for 10-12 hours to precipitate precipitates, centrifuge, and vacuum-dry at 50°C to obtain 40.4 g of brown polysaccharide with high immunomodulatory activity enzymatically hydrolyzed Lycium barbarum.

Embodiment 2

[0022] Example 2: Dry 300 grams of Lycium barbarum lees, grind it into primary powder, add 2.1 L of water, extract twice at 75° C. for 2 hours each time, and combine the filtrates. Concentrate under reduced pressure at 65°C to 1 / 10 of the original volume. Add 95% ethanol to the concentrated solution to make the alcohol content reach 80%, let stand, and precipitate out. The precipitate was vacuum-dried at 55°C, the obtained Lycium barbarum polysaccharide was dissolved in 400mL of water, 80mL of 0.007% saccharification enzyme solution was added, and hydrolyzed at 50°C for 20min. The hydrolyzate was boiled for 10 minutes to inactivate the enzyme. Add 95% ethanol for precipitation, let stand, centrifuge, and vacuum-dry at 55°C to obtain 57.1 g of brown high-immunomodulatory activity enzymatically hydrolyzed Lycium barbarum polysaccharide.

Embodiment 3

[0023] Example 3: Dry 250 grams of Lycium barbarum wine residue, grind it into primary powder, add 1.75 L of water, extract 3 times at 90° C. for 2 hours each time, and combine the filtrates. Concentrate under reduced pressure at 60°C to 1 / 10 of the original volume, add 95% ethanol to the concentrated solution to increase the alcohol content to 80%, let stand for 15 hours, and precipitate. The precipitate was vacuum-dried at 60°C, the obtained Lycium barbarum polysaccharide was dissolved in 350 mL of water, 70 mL of 0.01% saccharification enzyme solution was added, and hydrolyzed at 35°C for 15 minutes. The hydrolyzate was boiled for 10 minutes to inactivate the enzyme. Add 95% ethanol for precipitation, let stand, centrifuge, and vacuum-dry at 40°C to obtain 48.8 g of brown polysaccharide with high immunomodulatory activity enzymatically hydrolyzed Lycium barbarum.

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Abstract

The present invention provides a process method for producing high immunoregulatory activity lyceum polysaccharide by utilizing enzymolysis method and by using lyceum berry residue obtained after the lyceum berry is soacked in the white liquor as as raw material. Said process method includes the following steps: adopting water extraction alcohol precipitation method to extract primary polysaccharide from lyceum berry liquor residue, then adding amylase liquor whose concentration is 0.004-0.01%, making hydrolysis for 15-40 min at 30-50deg.C, boiling hydrolysate for 10 min, then adding 95% ethyl alcohol to make precipitation (alcohol precipitation concentration is 80%), removing low-molecular weight soluble saccharide, standing still for 10-18h, making centrifugal separation for 15 min by adopting 3000r/min, taking out precipitate and vacuum drying so as to obtain the invented brown high immunoregulatory activity enzymatic lycium polysaccharide.

Description

technical field [0001] The invention relates to an enzymatic hydrolysis technology of polysaccharides, in particular to a technology for preparing enzymolysis Lycium barbarum polysaccharide with high physiological activity. It changes the molecular weight of Lycium barbarum polysaccharide through enzymatic hydrolysis technology, so as to further improve the immunomodulatory activity of Lycium barbarum polysaccharide. Background technique [0002] Lycium barbarum is a traditional Chinese medicine, which has the effects of nourishing the liver and kidney, nourishing the liver and improving eyesight. With the progress of pharmacological and nutritional research on the main active components of wolfberry, people have realized that the main component of wolfberry is polysaccharide, which has a very significant regulatory effect on non-specific immunity and specific immunity. Inhibition of tumors, anti-aging, anti-fatty liver, lowering blood sugar, lowering blood lipids, anti-fat...

Claims

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Application Information

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IPC IPC(8): A23L1/30A23L1/09A23L1/212A61K36/815A61P37/02A23L33/105A23L33/125
Inventor 韩凤梅杜鹏郭小龙陈勇
Owner HUBEI UNIV
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