Turf grass stunt related gene GA20

A technology related to dwarfing genes and turfgrass, which is applied in the fields of genetic engineering, plant genetic improvement, recombinant DNA technology, etc.

Inactive Publication Date: 2008-03-12
BEIJING FORESTRY UNIVERSITY
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on this aspect in ...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Turf grass stunt related gene GA20
  • Turf grass stunt related gene GA20
  • Turf grass stunt related gene GA20

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0010] 1 Preparation of materials

[0011] Zoysia is taken from the Germplasm Resource Nursery of the Experimental Station of Turfgrass Research Institute of Beijing Forestry University in Dongbeiwang, Beijing. Its stolons are collected and brought back to the laboratory to be planted in flowerpots. They are carefully managed and used for experiments after growing young leaves.

[0012] 2 methods

[0013] 2.1 Extraction of total RNA and synthesis of double-stranded cDNA

[0014] Take the delicate leaves of Zoysia grass, quickly put them into liquid nitrogen and grind them into powder, and extract the total RNA according to the TRNzol kit from Tiangen Company; cDNA was synthesized using the AMV reverse transcription kit from Takara Company, in strict accordance with This manual operates.

[0015] 2.2 Obtaining the target gene fragment

[0016] 2.2.1 Acquisition of intermediate fragments

[0017] 2.2.1.1 Synthesis of degenerate primers

[0018] The degenerate primers GA20-1...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention clones GA20 oxydase gene from an excellent grass seed for lawn-Korea lawn grass and constructs the plant expression vector for the grass seed. By degenerating PCR and RACE method, GA20 oxydase gene is cloned from wild Korea lawn grass of China. The total length of the gene is 1311 bp, the code area length is 1109 bp, the code area is 96-1205 bp including 369 amino acid. The gene is of 65% homology with wheat and rice. The login no. on GenBank is DQ645453. In addition, the invention constructs the positive and reverse expression vector for the GA20 oxydase gene based on pC1303. The vector contains GUS report gene and hygromix marking gene, can be used for seed breeding in gene engineering of lawn grass so as to culture short transgenosis lawn grass.

Description

1. Technical field: [0001] The invention belongs to the field of plant biotechnology or the field of crop breeding. Specifically, related technologies related to the cultivation of Zoysia weed, RNA extraction, primer design, gene cloning and vector construction. Thus, the GA20 oxidase gene was obtained from Zoysia pratense, and the sense and antisense plant expression vectors of the gene were constructed. Two background technologies: [0002] The lawn has the functions of greening and landscaping, water and soil conservation, microclimate adjustment, viewing and sports, etc. It plays a decisive role in residents' rest, entertainment and landscape. At present, more than 4,000 hectares of lawns have been planted in Beijing, with an annual increase of more than 250 hectares, which has played an important role in beautifying Beijing's environment and improving the quality of life of the people in the capital. Yet lawn pruning and irrigation etc. expend a large amount of manpow...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/29C12N15/82C07K14/415
Inventor 韩烈保王月华何宇峰曾会明刘君
Owner BEIJING FORESTRY UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap