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Method of sterilized monitoring growing dynamic states of tissue cultured shoot root

A technology for monitoring plants and tissue culture seedlings, which is applied in the biological field and achieves the effects of wide application range, high efficiency and convenient operation

Inactive Publication Date: 2008-06-18
YUNNAN JIANGSHI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention utilizes image processing technology to monitor the growth dynamics of plant tissue culture seedling root, and this has not been reported at home and abroad

Method used

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  • Method of sterilized monitoring growing dynamic states of tissue cultured shoot root
  • Method of sterilized monitoring growing dynamic states of tissue cultured shoot root

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Inoculate the rootless tissue culture seedlings into the tissue culture bottle with the rooting medium for rooting culture, the rooting medium is 1 / 2MS plus 0.5mgl -1 IBA (indole butyric acid)+0.05mgl -1 NAA (naphthalene acetic acid) +0.1mgl -1 GA 3 (Gibberellin), the culture condition is that the temperature is 25±2°C, and the light intensity is 500 μmolm -2 the s -1 , The photoperiod is light 16h / dark 8h. Get a non-inoculated tissue culture seedling in a tissue culture bottle filled with rooting medium as a reference bottle, a section of 7cm long white line is put into this reference bottle in advance, and use the white line as a reference for root system image analysis. Then every other day, take the tissue culture bottle to be checked and the reference bottle, place them on the same horizontal position, keep the object distance at 17.7cm, use a 5 million pixel Kodak digital camera to take pictures from the bottom of the tissue culture bottle, and obtain and For...

Embodiment 2

[0021] Inoculate the rootless tissue culture seedlings into the tissue culture bottle with the rooting medium for rooting culture, the rooting medium is 1 / 2MS plus 0.5mgl -1 IBA (indole butyric acid)+0.005mgl -1 NAA (naphthalene acetic acid) +0.01mgl -1 GA 3 (Gibberellin), the culture condition is that the temperature is 25±2°C, and the light intensity is 500 μmolm -2 the s -1 , The photoperiod is light 16h / dark 8h. Get a non-inoculated tissue culture seedling in a tissue culture bottle filled with rooting medium as a reference bottle, a section of 7cm long white line is put into this reference bottle in advance, and use the white line as a reference for root system image analysis. Then every other day, take the tissue culture bottle to be checked and the reference bottle, place them on the same horizontal position, keep the object distance at 17.7cm, use a 5 million pixel Kodak digital camera to take pictures from the bottom of the tissue culture bottle, and obtain and F...

Embodiment 3

[0028] Inoculate the rootless tissue culture seedlings into the tissue culture bottle with rooting medium for rooting culture, the rooting medium is 1 / 2MS additional 5mgl -1 IBA (indole butyric acid)+0.005mgl -1 NAA (naphthalene acetic acid) +0.01mgl -1 GA 3 (Gibberellins, the culture conditions are that the temperature is 25±2°C, and the light intensity is 500 μmolm -2 the s -1 , The photoperiod is light 16h / dark 8h. Get a non-inoculated tissue culture seedling in a tissue culture bottle filled with rooting medium as a reference bottle, a section of 7cm long white line is put into this reference bottle in advance, and use the white line as a reference for root system image analysis. Then every other day, take the tissue culture bottle to be checked and the reference bottle, place them on the same horizontal position, keep the object distance at 17.7cm, use a 5 million pixel Kodak digital camera to take pictures from the bottom of the tissue culture bottle, and obtain and ...

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Abstract

Disclosed is a method for aseptically monitoring the growth dynamics of plant tissue culture seedling root system, which relates to the biotechnology field. The invention selects a linear object with known length to be positioned in a tissue culture container containing a rooting medium to serve as a reference bottle, the reference bottle and a tissue culture container to be detected grown with atissue culture root seedling are positioned on the same horizontal position, a camera is utilized to shoot the two bottles in the same picture from the bottom parts, each shooting keeps the same object distance and the same focal length and angle, the image analysis software can analyze and acquire the product pixel space of the linear object in the reference bottle and the root to be surveyed, and image analysis lengths of the tissue culture seedling root can be acquired by conversion; the image analysis lengths of the root to be surveyed can be acquired at regular intervals so as to draw a variation curve chart over the culture time, a fitting curve is gotten by the Logistic equation, and the growth dynamics of the tissue culture seedling root to be surveyed can be acquired according tothe fitted Logistic equation and the curve chart. The invention can realize the aseptic and harmless online detection to the growth of the tissue culture seedling root.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for aseptically monitoring the growth dynamics of the roots of plant tissue culture seedlings. Background technique [0002] Utilizing tissue culture to carry out rapid propagation is an effective propagation method for many flowers, vegetables and rare plant resources at present. Dynamically describing the growth and development of tissue cultured seedlings is of great significance in production and scientific research. The screening of medium, the regulation of environmental conditions, and the production of ordered flowers all require mastering the growth and development laws of tissue culture seedlings and understanding the influence of external factors on the growth and development of tissue culture seedlings. It is no longer possible to dynamically describe the growth and development of tissue culture seedlings by relying on visual observation or artificial destructiv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G7/00
Inventor 吴沿友许文祥李萍萍毛罕平赵玉国王纪章
Owner YUNNAN JIANGSHI TECH CO LTD