Polydatin medicinal composition containing polyvinyl pyrrolidone
A polyvinylpyrrolidone and composition technology, which is applied in the field of high-concentration polyvinylidene pharmaceutical compositions, can solve the problems of difficulty in storing drugs, difficulty in obtaining a clinical preparation scheme for polyvinylpyrrolidone injection, and unsuitable preparation of synthetic drug preparations.
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Embodiment 1
[0029] 1. Prescription
[0030] raw material
Dosage
PVP K15
30mg, 93mg, 158mg, 333mg, 529.4mg
pure water
3ml
[0031] Second, the method
[0032]Weigh 30mg, 93mg, 158mg, 333mg and 529.4mg of PVP K15 and add 3ml of pure water to make 10mg / ml, 31mg / ml, 52.7mg / ml, 111mg / ml and 176.5mg / ml PVP K15 aqueous solutions respectively. First, add polydatin to the prepared 5 reagent bottles to supersaturation, prepare another bottle of 31mg / ml PVP K15 solution, add 0.80mg polydatin, and prepare polydatin PVP with a known concentration of 0.267mg / ml K15 solution. After sonication at 40°C for 30 minutes, place at room temperature for 3 hours, then filter through a 0.45 μm microporous membrane, and dilute 300 times with pure water. Detected by HPLC, according to the peak area of 31 mg / ml PVP K15 polydatin aqueous solution of known concentration, calculate the saturation concentration of polydatin under different PVP K15 aqueous solution concentrat...
Embodiment 2
[0034] 1. Prescription
[0035] raw material
Dosage
PVP K30
33.7mg, 103.3mg, 176.3mg, 333mg
pure water
3ml
[0036] Second, the method
[0037] Weigh 33.7mg, 103.3mg, 176.3mg and 333mg of PVP K30 and add 3ml of pure water to make PVP K30 aqueous solutions of 11.2mg / ml, 34.4mg / ml, 58.8mg / ml and 111mg / ml respectively. First, add polydatin to the prepared 4 reagent bottles to supersaturation, prepare another bottle of 34.4mg / ml PVP K30 solution, add 0.80mg polydatin, and prepare polydatin with a known concentration of 0.267mg / ml PVP K30 solution. After sonication at 40°C for 30 minutes, place at room temperature for 3 hours, then filter through a 0.45 μm microporous membrane, and dilute 300 times with pure water. Detect with HPLC, calculate the saturation concentration of polydatin under different PVP K30 aqueous solution concentration according to the peak area of known concentration 34.4mg / ml PVP K30 polydatin aqueous solution.
Embodiment 3
[0039] 1. Prescription
[0040] raw material
Dosage
PVP K60
30mg, 61.2mg, 93mg, 125mg, 158mg, 225.8mg, 333mg
pure water
3ml
[0041] Second, the method
[0042] Weigh 30mg, 61.2mg, 93mg, 125mg, 158mg, 225.8mg and 333mg of PVP K60 and add 3ml of pure water to make 10mg / ml, 20.4mg / ml, 31mg / ml, 41.7mg / ml, 52.7mg / ml respectively , 75.3mg / ml and 111mg / ml PVP K30 aqueous solution. First, add polydatin to the prepared 7 reagent bottles to supersaturation, prepare another bottle of 34.4mg / ml PVP K60 solution, add 0.80mg polydatin, and prepare polydatin with a known concentration of 0.267mg / ml PVP K60 solution. After sonication at 40°C for 30 minutes, place at room temperature for 3 hours, then filter through a 0.45 μm microporous membrane, and dilute 200 times with pure water. Detect with HPLC, calculate the saturation concentration of polydatin under different PVP K60 aqueous solution concentration according to the peak area of known con...
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