Medicinal anoectochilus Formosan tissue culture one-step seedling establishment fast replication method

A technology for tissue culture and clematis, which is applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve the problems of long cultivation time, high cost, and lack of development of clematis, and achieve good growth results

Inactive Publication Date: 2008-07-09
浙江省中药研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the group cultivation of clematis seedlings is mainly realized through three stages of "induction of adventitious buds, bud proliferation and stro...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1) Medium preparation

[0035] (1) Preparation: take 1 / 2MS as the basic medium, add 6-BA2mg, NAA1.0mg, brassinolide 0.05mg, banana 100g, agar 6.5g and sucrose 25g per liter, the medium after mixing the above substances The pH value was adjusted to 5.6;

[0036] (2) Packing: Heat the prepared medium until the agar is completely dissolved, and evenly pack it into clean culture bottles while hot, about 50ml per bottle, and cover it after cooling slightly;

[0037] (3) Sterilization: put the culture bottle added with the culture medium into the autoclave for sterilization in time, the sterilization conditions are: sterilize at 121° C. and 105 kPa for 15 minutes, take out the culture bottle in time after sterilization, and cool it for later use.

[0038] 2) Explant treatment

[0039] (1) Cleaning: Select healthy plants that grow naturally and are free from diseases and insect pests, clean the plants with water added with a little soap powder, and then rinse with running wa...

Embodiment 2

[0051] 1) Medium preparation

[0052] (1) Preparation: take 1 / 2MS as the basic medium, add 6-BA 2mg, NAA 1.5mg, brassinolide 0.05mg, banana 100g, agar 6.5g and sucrose 25g per liter, and adjust the pH to 5.6;

[0053](2) Packing: Heat the prepared medium until the agar is completely dissolved, and evenly pack it into clean culture bottles while hot, about 50ml per bottle, and cover it after cooling slightly;

[0054] (3) Sterilization: put the culture bottle added with the culture medium into the autoclave for sterilization in time, the sterilization conditions are: sterilize at 121° C. and 105 kPa for 15 minutes, take out the culture bottle in time after sterilization, and cool it for later use.

[0055] 2) Explant treatment

[0056] (1) Cleaning: Select healthy plants that grow naturally and are free from diseases and insect pests, clean the plants with water added with a little soap powder, and then rinse with running water for 2 hours;

[0057] (2) Disinfection: Under as...

Embodiment 3

[0068] 1) Medium preparation

[0069] (1) Preparation: take 1 / 2MS as the basic medium, add 6-BA 2mg, NAA 1.5mg, brassinolide 0.05mg, banana 100g, agar 6.5g and sucrose 25g per liter, and adjust the pH to 5.6;

[0070] (2) Packing: Heat the prepared medium until the agar is completely dissolved, and evenly pack it into clean culture bottles, each bottle is about 50ml, and cover it after cooling slightly;

[0071] (3) Sterilization: put the culture bottle added with the culture medium into the autoclave for sterilization in time, the sterilization conditions are: sterilize at 121° C. and 105 kPa for 15 minutes, take out the culture bottle in time after sterilization, and cool it for later use.

[0072] 2) Explant

[0073] The more robust tissue culture sterile seedlings (plants) were selected as explants.

[0074] 3) Vaccination

[0075] Remove the leaves of the aseptic seedlings cultured under sterile conditions, cut the stems into several stem segments with a length of abou...

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PUM

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Abstract

The invention discloses a rapid propagation method of culturing medical anoectochilus roxburghii tissue through which seedling is formed in only one step. The invention mainly consists of the flowing steps: firstly, culture medium is prepared by three steps of preparation, prepackaging and sterilization; secondly, explant is treated and inoculated, which step has two conditions: when a natural growth plant is used as an explant, the step consists of three steps of cleaning, sterilizing and inoculation, and when a tissue culture sterilized seedling is used as an explant, the sterilized inoculation is directly carried out; thirdly, cultivation; and fourthly, transplantation. The invention uses the middle stem of an anoectochilus roxburghii plant, which is produced by subculture, as an explant. A stem section which contains a stem node is inoculated on culture medium under sterile condition. The results show that after the optimum culture medium is cultured for four months, the average seedling number of each explant is 4.8; the average net increased number of the leaves of each plant is 3.6; the average increased root number is 2.5 and the average net increased fresh weight is 525.6mg. The growing condition of the tissue culture seedling is quite good after being transplanted to substrate. The survival rate of plants achieves to above 95 percent after three months. The invention can greatly shorten the tissue culture seedling time of anoectochilus roxburghii, significantly improve the culture efficiency and reduce the seedling cost. The invention can be used as an important method of producing anoectochilus roxburghii seedlings.

Description

technical field [0001] The invention relates to a one-step seedling rapid propagation method for medicinal clematis tissue culture, which is mainly used for the tissue culture of medicinal clematis. Background technique [0002] Anoectochilus roxburghii (wall.) lindl] is a perennial herb of the family Orchidaceae, also known as golden silkworm, golden thread orchid, golden thread orchid, etc. It is a traditional and precious medicinal material in my country, known as "golden grass", "Miraculous medicine", "black ginseng" and other good names have a wide range of folk applications, and are widely used in the treatment of rheumatoid arthritis, high blood pressure, diabetes, kidney disease and other difficult and miscellaneous diseases, as well as physical fitness, recovery from physical weakness after illness, etc. On the other hand, especially in Zhejiang, Fujian, Taiwan and other provinces and Southeast Asia, it is regarded as a rare and precious medicinal material, especially...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00C12N5/04
Inventor 江建铭王志安俞旭平沈晓霞徐建中任江剑沈宇峰
Owner 浙江省中药研究所有限公司
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