Human cytomegalovirus recombinant protein and method for preparing the same

Abstract

The invention provides a human cytomegalovirus recombinant protein and the preparation method, relating to the field of genetic engineering technology and diagnostic reagent and vaccine development. The recombinant protein provided by the invention comprises subsequently 120 amino acids of the HCMV gp52 from the 261st to the 380th of the N-end, 54 amino acids of the pp150 from the 587th to the 640th of the N-end, 65 amino acids of the pp65 from the 361st to the 425th of the N-end, 44 amino acids of the gB from the 200th to the 243rd of the N-end, and 34 amino acids of the pp28 from the 95th to the 128th of the N-end from the N-end to the C-end. The human cytomegalovirus recombinant protein has a protein sequence with high specificity and strong immunogenicity that can improve the sensitivity and specificity of the test reagent, while the adoption of the multi antigenic determinant series recombinant protein technology can simplify the purification process and improve the working efficiency.

Description

technical field [0001] The invention relates to the fields of genetic engineering technology, diagnostic reagents and vaccine development, in particular to a human cytomegalovirus recombinant protein and a preparation method thereof. Background technique [0002] Human cytomegalovirus (Human Cytomegalovirus, referred to as HCMV) belongs to the herpesvirus β subfamily, is a DNA virus, its infection is widespread in the population, and the cytomegalovirus infection in our country is quite serious. Primary infection of women with CMV in early pregnancy is an important cause of fetal intrauterine infection and developmental defects. Among fetuses and newborns with primary infection of HCMV during pregnancy, 80% can lead to mental retardation, deformity and death [MiddeldorpJM.Jongsma J, Haar AT, et al. Detection of immunoglobulin M and G antibodies against cytomegalovirus early and late antigen by enzyme-linked immunosorbent assay. J Clin. Microbiol, 1984; 20(4): 763-771.]. HCM...

AI Technical Summary

Problems solved by technology

[0003] Currently, diagnostic methods for cytomegalovirus infection include exfoliated cells and histopathological examination, virus isolation, molecular hybridization test, and serological examination. The simple and rapid nature of the medical examination makes it widely used clinically. At present, the HCMV protein antigens used in most detection kits are mainly derived from virus culture or recombinantly expressed single protein fragments, or due to the low purity of protein antigens and The specificity is poor, resulting in false positives and reducing the specificity of detection; or because the single-fragment antigenic protein contains less antigenic determinants, the number of antibodies recognized is correspondingly less, which can easily cause false negatives and reduce the detection sensitivity. Preparation of detect