Liquid-solid combined cultivation reagent case for isolation culture, identification, quantification and drug sensitive test of antiureal and mycoplasma hominis and method of use thereof

Abstract

The invention relates to a liquid-solid combined culture kit for isolation and culture, identification, ration and drug sensitive tests of antiureal and mycoplasma hominis and an application method. The kit comprises drug sensitive laths and quantitative test laths, a liquid medium and a solid medium; the liquid medium and the solid medium are both weakly acidic, with a PH value about 6.0. Compared with the prior art, the liquid-solid combined culture kit for isolation and culture, identification, ration and drug sensitive tests of antiureal and mycoplasma hominis has the advantages of easy operation, reliable method, stable quality, easy observation and judgment of results and other advantages; meanwhile, the liquid-solid combined culture kit has good specificity and sensitivity, the liquid-solid combined kit can reduce the false positive rate by 5% to 10% or so, and the false negative rate by 2% to 5% or so; the liquid-solid combined culture kit basically eliminates the problem of the test result disturbances from the mixed fungi pollution, and has an important practical application value and a good market prospect.

Description

technical field [0001] The invention relates to a kit for biomedicine, in particular to a liquid-solid combined culture kit for separation and cultivation, identification, quantification and drug sensitivity test of urealyticum and mycoplasma hominis and an application method thereof. Background technique [0002] Mycoplasma is a kind of prokaryotic microorganism lacking cell wall, and more than 150 kinds of mycoplasma have been isolated so far. The mycoplasmas related to human diseases are mainly Ureaplasma urealyticum (hereinafter referred to as Uu), Mycoplasma hominis (hereinafter referred to as Mh) and Mycoplasma pneumoniae (Mycoplasma pneumoniae, Mp), among which Uu and Mh are recognized internationally as the cause of Pathogens of Genitourinary Tract Infection (Cao Yupu, edited by Ye Yuankang, Mycoplasma and Mycoplasma Disease, Beijing: People's Health Publishing House, 2000, 5). Mycoplasma research in my country, especially the research on the experimental diagnosis ...

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Problems solved by technology

Mycoplasma research in my country, especially the research on the experimental diagnosis of pathogenic mycoplasma, started late, the method is backward, and because of the low positive rate of mycoplasma culture and the contamination of miscellaneous bacteria, it is prone to false positives and many other problems, so it cannot be widely used in clinical diagnosis.

For the cultivation and separation of mycoplasma abroad, there are commercialized Difco, Oxoid dry base, and the finished medium of French bio-Mérieux. Domestic laboratories use mycoplasma isolation medium, some imported from abroad, but the price is expensive; some Self-prepared, but the quality is unstable, which directly affects the success or failure of mycoplasma isolation and culture (TullyJG, et al.Methods in mycoplasmology, Vol I, II, New York: Academic press, 1983; Tully JG, et al.Molecular and diagnostic procedures in mycoplasmologydiagnostic procedures, Vol I, New York: Academic press, 1996)