Liquid-solid combined cultivation reagent case for isolation culture, identification, quantification and drug sensitive test of antiureal and mycoplasma hominis and method of use thereof
A technology of Mycoplasma hominis and Mycoplasma urealyticum, which is applied in the directions of biochemical equipment and methods, determination/inspection of microorganisms, etc., can solve the problems affecting the success or failure of the separation and culture of Mycoplasma, the positive rate of Mycoplasma culture is low, the quality is unstable, etc. Important practical value, good market prospects, good specificity and sensitivity
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[0023] Example 1
[0024] A liquid-solid combined culture kit for urealytic and Mycoplasma hominis separation culture, identification, quantification and drug sensitivity test, including drug sensitivity and quantitative test strips, transparent sealing film, bottled liquid medium, and slatted solid phase culture The medium is light yellow, transparent, clear liquid or solid, and its component formula is as follows:
[0025] Base 700ml,
[0026] 200ml horse (or bovine) serum,
[0027] 25% yeast extract 100ml,
[0028] 40% urea 15ml,
[0029] 30% L-arginine 33ml,
[0030] 0.4% phenol red 5ml,
[0031] 10000IU / ml penicillin 50ml,
[0032] The formula of the basic base contains 50g of beef heart powder, 10g of peptone, and 5g of NaCl per 1000ml of the base. Adjust the pH to 6.0 in the 1000ml medium, and add 10-12g agar powder to the solid phase medium;
[0033] The drug sensitivity and quantitative test strip has 26 small holes: C+ hole is a positive control hole, C- hole is a negati...
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[0037] Example 2
[0038]An application method of a liquid-solid combined culture kit for urealytic and Mycoplasma hominis separation culture, identification, quantification and drug sensitivity test, the method includes the following steps:
[0039] (1) Take the specimen to be inspected: such as urethral secretions with a sterile cotton swab, and semen with a dropper;
[0040] (2) Inoculate the specimens to be inspected on liquid medium and solid medium by conventional methods: for example, liquid specimens are directly added to liquid medium and smeared on the surface of solid medium;
[0041] (3) Use a dropper to suck up 0.1ml of standard cultures of Ureaplasma urealyticum and Mycoplasma hominis and add them to well C+ of the drug susceptibility and quantitative test strip. Take 0.1ml of liquid culture medium and add it to well C-, and take samples with specimens to be tested. Add 0.1ml of liquid-phase culture medium to Uu holes, Mh holes, and drug sensitivity test holes, seal ...
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[0047] Example 3
[0048] A growth performance test method:
[0049] Strain: Mycoplasma hominis (ATCC 23114) 37℃24h culture,
[0050] Ureaplasma urealyticum (ATCC 27618) 37℃24h culture,
[0051] Equipment: incubator (36℃±1℃), sterile straw
[0052] Steps: (1) Take the tested medium,
[0053] (2) Use a sterile straw to suck up 0.1ml of the above-mentioned cultures of Mycoplasma hominis and Ureaplasma urealyticum, add them to the liquid and solid medium, and then place them in an incubator (36°C±1°C) for cultivation.
[0054] (3) Culture of Mycoplasma hominis for 48h and Ureaplasma urealyticum for 24h, and take out the observation results.
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