Method for producing saturated and unsaturated alpha, omega-dicarboxylic acid with microbial transformation of oil and fat
A technology for microbial transformation and dicarboxylic acid, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve expensive and other problems
Inactive Publication Date: 2008-09-24
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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Abstract
The invention discloses a method of utilizing microbial transformation grease to produce saturated and unsaturated Alpha- or Omega- dicarboxylic acid, in particular to a method of producing 9-Octadecadienoic acid (Delta<9>DC18). The microorganism to be used is ou-3 mutant strain and belongs to Candida tropicalis. The invention is characterized in that after the microorganism strain is inoculated into the culture medium of containing a substrate of oleic alcohol, oleic acid and methyl oleate, cell growth of the system is mainly controlled and grease is also transformed to produce binary acid at the first stage; the reaction system is mainly controlled on acid production and also on growth of some cell at the second stage; the system is only controlled on acid production and without the cell growth. The method is used to transform the oleic alcohol to produce 9-Octadecadienoic acid (Delta<9>DC18), the output of Delta <9>DC18 reaches 110.5 grams per Liter in 16 liter fermentation tank for about transformation of 144 hours.
Application Domain
FungiMicroorganism based processes +1
Technology Topic
GramGrowth cell +17
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- Experimental program(5)
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