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Method for producing saturated and unsaturated alpha, omega-dicarboxylic acid with microbial transformation of oil and fat

A technology for microbial transformation and dicarboxylic acid, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve expensive and other problems

Inactive Publication Date: 2008-09-24
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, four kinds of precious animal fragrances (musk, dragon birth incense, civet incense, castoreum) can only be obtained from wild animals, for example, musk can only be obtained from the testicles of civets, musk deer, etc., which is extremely expensive

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0020] (1) Take an inoculation loop of ou-3 strain, spread it on the wort solid slope of a Φ15×180 large test tube, and cultivate it at 30°C for two days.

[0021] (2) Take one branch of the above-mentioned bacterial strain, insert it into a 250 mL Erlenmeyer flask containing 30 mL of wort seed medium, and cultivate it at 30° C. on a rotary shaker at 220 rpm for 46 hours.

[0022] (3) Into a 500mL Erlenmeyer flask containing 15mL of fermentation medium, add 3.5mL of the above seed solution, ferment on a rotary shaker at 220 rpm for 4 days, and adjust the pH to 7.5-8.0 with NaOH every 24 hours. KH in the fermentation medium 2 PO 4 8g / L, KNO 3 4g / L, Tween 603g / L, yeast extract 2g / L, corn steep liquor 2.5g / L, NaCl 1g / L, urea 1.8g / L, sucrose 20g / L, acrylic acid 1g / L, oleyl alcohol 150mL / L, Prepared with tap water, pH 7.1, sterilized at 110°C for 30 minutes. After the fermentation, the pH was adjusted to 3 with 6mol HCl. Extract with an organic solvent, first remove the remain...

example 2

[0024] According to the method of example 1, just raw material uses lauric acid, the result DC 12 Yield was 46.5g / L.

example 3

[0026] According to the method of example 1, just raw material uses palmitoleic acid, the result 9 alkene-hexadecandioic acid (Δ 9 DC 16 ) yield is 43g / L.

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Abstract

The invention discloses a method of utilizing microbial transformation grease to produce saturated and unsaturated Alpha- or Omega- dicarboxylic acid, in particular to a method of producing 9-Octadecadienoic acid (Delta<9>DC18). The microorganism to be used is ou-3 mutant strain and belongs to Candida tropicalis. The invention is characterized in that after the microorganism strain is inoculated into the culture medium of containing a substrate of oleic alcohol, oleic acid and methyl oleate, cell growth of the system is mainly controlled and grease is also transformed to produce binary acid at the first stage; the reaction system is mainly controlled on acid production and also on growth of some cell at the second stage; the system is only controlled on acid production and without the cell growth. The method is used to transform the oleic alcohol to produce 9-Octadecadienoic acid (Delta<9>DC18), the output of Delta <9>DC18 reaches 110.5 grams per Liter in 16 liter fermentation tank for about transformation of 144 hours.

Description

technical field [0001] The present invention relates to the production of saturated and unsaturated α, ω-dicarboxylic acids by microbial conversion of oil, especially the conversion of oleic acid, methyl oleate and oleyl alcohol in oil to produce 9-octadecanedioic acid (Δ 9 DC 18 )Methods. Background technique [0002] Long-chain dibasic acids are important raw materials for synthetic perfumes, nylon engineering plastics, hot-melt adhesives, coatings, lubricating oils, resins and medicines. Saturated and unsaturated long-chain dibasic acids are important raw materials for chemically synthesizing famous and precious fragrances such as musk fragrance and civet fragrance. In the past, four kinds of precious animal fragrances (musk, dragon birth incense, civet incense, and castoreum) could only be obtained from wild animals. For example, musk can only be obtained from the testicles of civets and musk deer, which is extremely expensive. Today, the main active ingredient with p...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12N1/16C12R1/74
Inventor 陈远童郝秀珍傅深展席悦
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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