Milk derived composition and use to enhance muscle mass or muscle strength
一种肌肉力量、组合物的技术，应用在补充进行抗阻运动训练的个体营养需求领域，能够解决不具有治疗作用、蛋白质不易被肠吸收、不能承受消化系统严苛环境等问题
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Embodiment 1
[0088] Embodiment 1: clinical trial
[0089] A clinical trial was implemented in which 20 young men participated in a randomized double-blind resistance training program for three months. Whey growth factor extract was prepared according to the method generally described in Australian Patent No. 645589 (PCT / AU91 / 00303) (described in more detail above). Each whey protein formulation contains artificial sweeteners (Nutrasweet TM , Nutrasweet Company, USA). Whey protein preparations were taken immediately after each exercise session, and each subject completed exercise three times a week under supervision.
[0090] Subjects were randomly assigned to one of three supplementation groups:
[0091] Group A: NatraPro (WPI); 20g / dose; n=7
[0092] Group B1: NatraPro (WPI) plus WGFE; 20g WPI plus 1g WGFE / dose; n=6
[0093] Group B2: NatraPro (WPI) plus WGFE; 20g WPI plus 2g WGFE / dose; n=7
[0094] NatraPro TM Typical components of a WPI include:
[0095] Moisture 5.0%
[0096]...
Embodiment 2
[0131] Example 2: Effect of WGFE on the growth of L6 myoblasts
[0132] In vitro myocyte growth studies were performed in which L6 myoblasts were cultured in the presence of WGFE (LP), colostrum, 10% fetal calf serum (FCS), or medium alone (unstimulated). Whey growth factor extract was prepared according to Example 1.
[0133] Figure 4A : L6 myoblast growth assay in response to stimulation with concentrations of 0.04-5 mg / ml WGFE or colostrum in 48 hours. Each point represents the arithmetic mean±SEM of triplicate determinations.
[0134] There was an approximately 2-fold increase in cell number when stimulated with 5 mg / ml WGFE for 48 hours. Include 10% FCS as a positive control. Colostrum produced by Murray Goulburn Co-Op Company Ltd. was also included for comparison purposes.
[0135] Figure 4B : L6 myoblast growth assay in 48 hours measuring dose response to WGFE up to 10 mg / ml. Each point represents the arithmetic mean±SEM of triplicate determinations.
[0136] ...
Embodiment 3
[0139] Example 3: Effects of WGFE and WPI on Fibroblast Growth
[0140] In vitro fibroblast growth studies were performed in which BalbC 3T3 fibroblasts were cultured in the presence of WGFE, WPI, colostrum and other milk fractions, 10% FCS ('+ve' control) or media alone ('Nil'). Whey growth factor extract was prepared according to Example 1. Commercially available from MurrayGoulburn CoOp Company Ltd. under the trade name NatraPro TM The WPI.
[0141]BalbC 3T3 fibroblasts were cultured for 48 hours in response to colostrum, 100 mg / ml WPI, 100 mg / ml WGFE, or various milk fractions as described above. Graphed values represent the arithmetic mean±SEM of mean responses obtained in 3 independent experiments. This assay showed that WGFE was much more effective in stimulating cell growth than WPI alone ( Figure 5 ).
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