Visualized oxygen sensing detection biosensor preparation method
A biosensor and oxygen sensing technology, applied in the field of biosensors, can solve the problems of low sensitivity of colorimetric oxygen sensors, achieve high resolution, good stability, and realize the effect of visual detection
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Embodiment 1
[0017] Mix TEOS, APTMS, ultrapure water and methanol at a volume ratio of 1:8.8:20:101.3, take 13.10 mL into a polytetrafluoroethylene beaker, stir at room temperature for 1 hour; seal the polytetrafluoroethylene beaker with parafilm , perforate a few small holes on the sealing film to facilitate the volatilization of methanol, place the polytetrafluoroethylene beaker in a 30°C incubator for 48h; then add 1mL of 2×10 -3 mol / L green quantum dot solution and 0.3mL Cd precursor solution (CdCl 2 Mix with thioglycolic acid at a molar ratio of 1:2.4 and dilute with ultrapure water to a concentration of 2×10 -3 mol / L, use 1.0mol / L NaOH to adjust the pH of the solution to 9 to obtain the Cd precursor solution), put the mixed solution in a 30°C incubator and store it in the dark for 4h, then take 40μL on a glass slide (48×12.4×0.9 , mm) was uniformly coated on the film, and placed in a 30°C incubator to avoid light and dry to obtain a quantum dot layer with stable luminescence.
[00...
Embodiment 2
[0020] Mix TEOS, APTMS, ultrapure water and methanol at a volume ratio of 1:8.8:20:101.3, take 13.10 mL into a polytetrafluoroethylene beaker, stir at room temperature for 1 hour; seal the polytetrafluoroethylene beaker with parafilm , perforate a few small holes on the sealing film to facilitate the volatilization of methanol, place the polytetrafluoroethylene beaker in a 30°C incubator for 48h; then add 1mL of 2×10 -5 mol / L green quantum dot solution and 0.3mL Cd precursor solution (CdCl 2 Mix with thioglycolic acid at a molar ratio of 1:2.4 and dilute with ultrapure water to a concentration of 2×10 -5 mol / L, use 1.0mol / L NaOH to adjust the pH of the solution to 10 to obtain the Cd precursor solution), put the mixed solution in a 30°C incubator and store it in the dark for 4h, then take 60μL on a glass slide (48×12.4×0.9, mm) evenly, and placed in a 30°C incubator to avoid light and dry to obtain a quantum dot layer with stable luminescence.
[0021] Measure TMOS and DiMe-...
Embodiment 3
[0024] Mix TEOS, APTMS, ultrapure water and methanol at a volume ratio of 1:8.8:20:101.3, take 13.10 mL into a polytetrafluoroethylene beaker, stir at room temperature for 1 hour; seal the polytetrafluoroethylene beaker with parafilm , perforate a few small holes on the sealing film to facilitate the volatilization of methanol, place the polytetrafluoroethylene beaker in a 30°C incubator for 48h; then add 1mL of 5×10 -4 mol / L green quantum dot solution and 0.3mL Cd precursor solution (CdCl 2 Mix with thioglycolic acid at a molar ratio of 1:2.4 and dilute with ultrapure water to a concentration of 5×10 -4mol / L, use 1.0mol / L NaOH to adjust the pH of the solution to 11 to obtain the Cd precursor solution), put the mixed solution in a 30°C incubator and store it in the dark for 4h, then take 50μL on a glass slide (48×12.4×0.9 , mm) was uniformly coated on the film, and placed in a 30°C incubator to avoid light and dry to obtain a quantum dot layer with stable luminescence.
[00...
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