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Microoptic glucose detector

A detector and light source technology, applied in the field of fully integrated fluorescence detection microsystems, can solve problems such as unused fluorescence detection units or fluorescence detection microsystems

Inactive Publication Date: 2008-11-12
HONEYWELL INT INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Self-assembled PBA monolayers have also been used to develop sensitive and selective electrochemical and optical sugar sensors, but until now they have not been used in large-scale fluorescent detection cells or fluorescent detection microsystems

Method used

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  • Microoptic glucose detector
  • Microoptic glucose detector
  • Microoptic glucose detector

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] according to figure 1 with figure 2 The synthetic route shown in to prepare the fluorescent compound of formula II (where n and m are each 1, p is 2): first, the phenylboronic acid anhydride of formula 1 is prepared as follows: o-bromotoluene and magnesium (1.1 equivalent) in React in diethyl ether at 25°C. To a solution of trimethylboronate (10 equivalents) in diethyl ether was added dropwise a Grignard reagent at -78°C. The mixture was stirred for an additional 2 hours, then allowed to warm to room temperature and stirred for an additional 2 hours. Diethyl ether was removed under reduced pressure and the solid was recrystallized from water. The product phenylboronic acid was dried overnight in a vacuum oven to produce phenylboronic anhydride.

[0066] Phenylboronic anhydride was mixed with NBS (N-bromosuccinimide) (1.1 equivalents) and catalytic AIBN (azoisobutyronitrile) in carbon tetrachloride as solvent. The mixture was refluxed for 2 hours under the irradiat...

Embodiment 2

[0071] Self-assembled monolayers of the compound of formula II were prepared by immersing micropatterned gold platelets in a solution of the compound of formula II for 15 hours. The solution concentration was 1.0 mM, and the solvent was 9:1 THF:methanol. Fixation methods were monitored by SPR spectroscopy. After the SAM was formed, the pellet was rinsed with methanol and then dried under nitrogen.

[0072] Microscope glass coverslips were used as the substrate for the gold flakes. coverslips with Piranha solution (30% H 2 o 2 : Concentrated H 2 SO 4 1:3) for 15 seconds, with Milli- Rinse carefully with grade water. The coverslip was then dried under a stream of nitrogen and placed in a vacuum evaporator. A gold film (about 500 nm thick) was deposited on the coverslip using a Polaron E5000 sputtering system. Hold the sputtering system for 180 seconds under the following conditions: 2.0 x 10 -2 mbar and 20mA.

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PUM

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Abstract

Disclosed is a compound that fluoresces in the presence of saccharides, the compound having the formula: wherein F designates a fluorophore, R is a lower aliphatic or aromatic group, n and m are each 0, 1, or 2, n+m is the integer 2 or 3, p is an integer of 1 to about 30, and the moieties [CH2CH2]p and the benzene ring attached to the boron atom are substituted or unsubstituted. The present invention also provides a fully-integrated microfluidic system for detection of the fluorescence which comprises a light source; a self-assembled monolayer of a compound of the above formula coated on a gold surface that fluoresces in the presence of a saccharide; a light processor; and a detector.

Description

field of invention [0001] The present invention relates to a novel fluorescent compound, in particular to a novel fluorescent compound suitable for the detection of sugars or sugars such as glucose, to a new method for sugar detection using such fluorescent compounds, and to a novel fully integrated Fluorescence detection microsystem. Background of the invention [0002] A variety of assay methods have been developed to detect and quantify analytes contained in biological samples. Most assay methods in use today rely on specific binding reactions between the analyte and assay reagents. Analytes can be large complex molecules such as proteins, viruses, viral antigens, bacterial cells, cell surface receptors, enzymes, hormones, polysaccharides, glycoproteins, lipoproteins, or small haptenic molecules such as peptides , certain hormones, therapeutic drugs, and drugs of abuse are just examples. [0003] Binding assays can be divided into two broad categories based on their fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07F5/02A61B5/00
CPCB82Y15/00C07F5/025B82Y30/00
Inventor 古元东张春波
Owner HONEYWELL INT INC