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Flavonoid application

A technology for flavonoids and baicalein, which is applied in the application field of flavonoids, and can solve the problem that it is difficult to meet the analysis needs of analytes with different properties and the like

Active Publication Date: 2008-11-19
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is difficult to meet the analysis needs of analytes with various properties in daily work, especially those samples that are unstable in acidic environments, and it is difficult to obtain ideal results by using MALDI-TOF mass spectrometry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Weigh each 1mg of quercetin, catechin, baicalein or wogonin, and weigh 3 parts of each, and then dissolve each part in 100μL methanol, ethanol or acetone to obtain 12 kinds The solution was shaken until it was fully dissolved, and the supernatant was centrifuged for storage;

[0028] Take 1 μL of each of the 12 solutions of quercetin, catechin, baicalein or wogonin, and spot them on a 100-hole stainless steel target. After air-drying, the sample target is sent to the ion source for MALDI-TOF mass spectrometry experiment .

[0029] The obtained spectrum shows that the solvent has little effect on the peaking of the four flavonoids, and the peaking is relatively simple, which meets the necessary conditions for becoming an effective matrix. Figure 1-4 is the MALDI-TOF mass spectrum of quercetin, catechin, baicalein or wogonin in methanol solution. In Figure 1-3, there are three main peaks. From left to right, they are the protonation peak, protonation peak and sodium ionizatio...

Embodiment 2

[0030] Example 2: Weigh 1 mg of DHB and THAP respectively, and weigh 3 parts of each, and dissolve each part of DHB and THAP in 100 μL methanol, ethanol or acetone to obtain 6 solutions, and then shake them to full Dissolve, centrifuge to take the supernatant and save;

[0031] Polysaccharides use ultrapure water (please give the so-called ultrapure water characterization index value!) to make a 4mg / mL solution, and use the 12 solutions of quercetin, catechin, baicalein or wogonin in Example 1 respectively For comparison and analysis with 6 solutions of DHB and THAP as the matrix, take 1μL of all the matrix solution and place it on a 100-hole stainless steel target. After air-drying, place 1μL of polysaccharide solution on the upper layer of the matrix. The sample target is sent to the ion source and analyzed by MALDI-TOF mass spectrometry. The results are shown in Table 1.

Embodiment 3

[0033] The organic-inorganic complex uses chloroform as the solvent to prepare a 5 mg / mL solution. The 12 solutions of quercetin, catechin, baicalein or wogonin in Example 1 and the 6 solutions of DHB and THAP in Example 2 were used as substrates for comparative analysis. Take 1μL of all the substrate solutions. Spot on a 100-hole stainless steel target. After air-drying, spot 1μL of organic-inorganic complex solution on the upper layer of these substrates. After air-drying, the sample target is sent to the ion source and analyzed by MALDI-TOF mass spectrometry. The results are shown in Table 1.

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Abstract

The invention relates to an application of a flavonoid. The invention provides the flavonoid such as quercetin, catechin, baicalein or wogonin as a matrix for desorbing the ionization time of flight mass spectrometry by means of matrix-assisted laser. A benzene ring of the flavonoid contains adjacent hydroxyls and carbonyls, and a stable six-membered or five-membered ring structure can be formed between the hydroxyls and the carbonyls or between the hydroxyls in the form of hydrogen bonds in a molecule, thereby facilitating the proton exchange between the flavonoid as the matrix and an analyte. The flavonoid, as the matrix, can be efficiently and rapidly applied in analysis of samples which are difficult to be characterized such as polysaccharide, electrostatic compounds, organic and inorganic compounds and C60 derivatives due to the weak acidity, and can solve the difficult problems of molecular weight and structure analysis of certain samples which may have dissociation of compounds or even breakdown of chemical bonds arising from poor stability and vulnerability to environmental impact in commonly used acidic matrix.

Description

Technical field [0001] The invention relates to the application of flavonoids. technical background [0002] The matrix is ​​the most important part of MALDI-TOF mass spectrometry and the key to the success of MALDI-TOF mass spectrometry. The discovery and use of efficient matrices can expand the application range of MALDI-TOF mass spectrometry. Since the 1980s, Tanaka and Hillenkamp used a mixture of metal powder and glycerin and niacin as the matrix and successfully analyzed peptides and proteins. The selection and use of the matrix and the mechanism of the matrix have become hotspots of research. [0003] Summarizing the exploration of MALDI-TOF mass spectrometry matrix by many researchers over the years, it is found that an effective matrix should have the following characteristics: [0004] (1) It can effectively dilute and disperse sample molecules; [0005] (2) The matrix has strong absorption in the laser wavelength region used (the commonly used N 2 The laser wavelength ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/38G01N27/64
Inventor 刘淑莹路显锋宋凤瑞刘志强崔勐
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI