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Fermentation method for pleuromulin

A technology of pleuromutilin and fermentation method, which is applied in the field of pleuromutilin fermentation, can solve the problems that the production capacity of the strain is not fully utilized, the utilization rate of the fermentation tank is only 75%, and the utilization rate of the fermentation tank is low.

Inactive Publication Date: 2011-02-16
江苏赛奥生化有限公司
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The utilization rate of the fermenter in the batch fermentation method is low, the utilization rate of the fermenter is only about 75%, and the production capacity of the strain has not been fully utilized

Method used

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Examples

Experimental program
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Embodiment Construction

[0008] A fermentation method for pleuromutilin, which comprises the steps of inoculating bacterial strains on the slant surface of a test tube, after 15 days of culturing, inserting the grown slanted bacterial strains into a shaking flask, and after 8 to 9 days of shaking and cultivating on a shaking table, then inserting the Seed tank, the amount of inoculation connected to the seed tank is 15% of the liquid capacity of the fermenter. After three-stage cultivation, self-fermentation, fermentation for 30 to 50 hours until the reducing sugar drops to 1.0%, continuous flow of 30% to 40% V / V glucose liquid, the glucose content in the fermentation liquid is 0.8-1.0%, and the pH value of the fermentation liquid is adjusted to 6.8-7.0 with NaOH solution; when the fermentation reaches 120-150 hours, observe the growth status of the mycelium, when the mycelium is beautiful When the blue staining changes from dark to light, add corn steep liquor with a liquid capacity of 1.0% of the fe...

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Abstract

The invention discloses a fermentation method for pleuromutilin. A feeding belt fed-batch fermentation method is adopted, and the strain culture in a seeding tank is changed from second grade to third grade. The method can effectively improve the utilization rate of a fermentation tank and the production capacity of strains.

Description

Technical field: [0001] The present invention relates to a fermentation method of pleuromutilin. Background technique: [0002] At present, in the fermentation production process of pleuromutilin, an intermediate of Vonimulin, each factory adopts the fed-batch fermentation method, that is, the liquid nitrogen cryovial strain is inoculated to the inclined surface of the test tube, and after 15 days of culture, the growth Good slanted strains are inserted into shake flasks, shaken for 8-9 days, and then inserted into seed tanks. Usually, secondary seed tanks are used for cultivation, which takes four days. To meet the needs of the fermentation process, continuously add glucose, soybean oil, alkali, water and other materials, ferment for about 300 hours, and put the titer at 9000-10000u / ml. The batch fermentation method has a low utilization rate of the fermenter, and the utilization rate of the fermenter is only about 75%, and the production capacity of the strain has not bee...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/38C12R1/645
Inventor 陈剑慧韩勤更冒永松贾永飞沈春梅
Owner 江苏赛奥生化有限公司