Prunella plant extract, preparation method and use thereof

A technology of Prunella vulgaris and extracts, which is applied in the direction of drug combination, medical formula, plant raw materials, etc., can solve the problems of unclear material-basic correlation, etc., and achieve the effects of environmental friendliness, simple process steps, and high yield

Active Publication Date: 2008-12-31

上海硕方医药科技有限公司

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[0005] Although there are many reports on the pharmacological effects of Prunella vulgaris, the material basis of its ...

Abstract

The invention relates to prunella plant extract which comprises total triterpene extracts forming the active ingredients; in the total triterpene extracts, the content of ursolic acid is 10.0-45.0wt%, and the content of total triterpene is 50.5-98.0wt%. The prunella plant extract also contains total phenolic acid extracts forming active ingredients; in the total phenolic acid extracts, the content of rosmarinic acid is 14.0-65.0wt%, and the content of total phenolic acid is 70.5-99.0wt%. The invention also relates to a preparation method of the composition and the application in the medicine.

Application Domain

Sexual disorderCardiovascular disorder +1

Technology Topic

ChemistryPrunella +6

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Experimental program(26)

Example Embodiment

[0066] Example 1

[0067] Preparation method of total triterpene extract in Prunella vulgaris plant extract:

[0068] (1) 500g of Prunella vulgaris ears, plus 80% ethanol by weight percentage, extract three times by heating and refluxing, each time 3500ml, the time is 2, 1.5, 1 hour; combine the three extracts, and use the method of reduced pressure and concentration to recover the ethanol , Concentrate to about 500ml; let stand overnight at room temperature, filter, filter residue A and filtrate A are separately used, and filter residue A is about 12g after drying;

[0069] (2) Filter residue A is dissolved in about 100ml of ethanol with a concentration of 80% by weight, add 50g of 100 mesh diatomaceous earth to mix the sample for adsorption, evaporate to dryness under reduced pressure for column chromatography, elute with 500ml of petroleum ether, recover petroleum ether, and discard the residue , And then eluted with 500ml ethanol to collect the total triterpene eluate;

[0070] (3) Recover ethanol from the total triterpene eluate under reduced pressure to obtain 4.6g of the residue, dissolve it with 250ml of 90% ethanol by weight percentage, add acetic acid to adjust the pH to 5, add 5g of activated carbon to heat and reflux for 1 hour; filter, The ethanol is recovered from the filtrate to obtain 2.8g of total triterpene extract, of which ursolic acid content is 32%, and total triterpene content is 68%.

Example Embodiment

[0071] Example 2

[0072] Preparation method of total triterpene extract in Prunella vulgaris plant extract:

[0073] (1) 100g of Prunella vulgaris ears, with a weight percentage of 80% ethanol, were extracted three times by ultrasonic method, 700ml each time, and the time was 2, 1.5, and 1 hour. Combine the three extracts, and use the method of concentration under reduced pressure to recover the ethanol until there is no alcohol; let stand overnight at room temperature, filter, filter residue A and filtrate A are used separately, and filter residue A is about 2.5g after drying;

[0074] (2) The filter residue A is dissolved in about 20ml of ethanol with a concentration of 80% by weight, add 10g of 100 mesh diatomaceous earth to mix the sample for adsorption, evaporate the ethanol, evaporate to dryness under reduced pressure for column chromatography, eluting with 100ml of petroleum ether, and recover the petroleum Ether, discard the residue, and then eluted with 100ml ethanol to collect the total triterpene eluate;

[0075] (3) Recover ethanol from the total triterpene eluate under reduced pressure to obtain the residue 0.95g, dissolve it in 50ml ethanol with a concentration of 90% by weight, add acetic acid to adjust the pH to 5, add 1g activated carbon to heat and reflux for 1 hour; filter, Ethanol was recovered from the filtrate, and the residue was washed twice with water, about 10 ml each time, filtered and dried to obtain 0.59 g of total triterpene extract, in which ursolic acid content was 30% and total triterpene content was 66%.

Example Embodiment

[0076] Example 3

[0077] Preparation method of total triterpene extract in Prunella vulgaris plant extract:

[0078] (1) 100g of Prunella vulgaris ears, plus 80% ethanol by weight percentage, extract three times by ultrasonic method, 700ml each time, the time is 2, 1.5, 1 hour; combine the three extracts, and use the method of reduced pressure concentration to recover the ethanol Until there is no alcohol smell; let stand overnight at room temperature, filter, filter residue A and filtrate A are used separately, and filter residue A is about 2.6g after drying;

[0079] (2) Filter residue A is dissolved in about 80ml of 100% ethanol by weight percentage, add 10g of 100-mesh diatomaceous earth to mix the sample for adsorption, evaporate the ethanol, evaporate to dryness under reduced pressure for column chromatography, eluted with 100ml of petroleum ether, and recover the petroleum Ether, discard the residue, and then eluted with 100ml ethanol to collect the total triterpene eluate;

[0080] (3) Recover ethanol from the total triterpene eluate under reduced pressure to obtain the residue 0.98g, dissolve it with 50ml ethanol with a concentration of 90% by weight, add acetic acid to adjust the pH to 5, add 1g activated carbon to heat and reflux for 1 hour; filter, Ethanol was recovered from the filtrate, and the residue was washed twice with water, about 10 ml each time, filtered and dried to obtain 0.63 g of total triterpene extract, in which the content of ursolic acid was 29% and the content of total triterpenes was 63%.

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Prunella plant extract, preparation method and use thereof

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