Unlock instant, AI-driven research and patent intelligence for your innovation.

DNA molecular identification method of Haliotis sieboldii and Haliotis discus Hannai hybrid

A technology for DNA molecules and rump abalone, which is applied in the field of molecular identification, can solve the problems of few types of enzymes, low polymorphism, complicated procedures, etc., and achieves the effects of high accuracy, stable results and simple statistics.

Inactive Publication Date: 2009-01-28
XIAMEN UNIV
View PDF1 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the technical requirements are strict, the procedure is complicated, the recognition is not intuitive, and the expression of isozymes is related to the tissue and developmental stage, so the polymorphism is low, the types of enzymes available are less, the repeatability is not good enough, and the requirements are high The laboratory conditions are not easy to analyze a large number of samples

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • DNA molecular identification method of Haliotis sieboldii and Haliotis discus Hannai hybrid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] see figure 1 , using West's abalone ♀ and wrinkled pan abalone Hybridization is carried out to obtain hybrid seedlings, and the genome DNA of the hybrid species of Siberian abalone and Panbao wrinkle is extracted by the phenol-chloroform method. Using the hybrid DNA as a template, carry out PCR amplification reaction: the total volume of the PCR amplification reaction system is 25 μl, and its various components and final concentrations are: Buffer 10mM, dNTP 0.2mM, Mg 2+ 1.5mM, primer pair 0.2mM, Taq 1U, genomic DNA 50ng, make up to 25μl with double distilled water; the primer sequence is:

[0023] Primer Awb002:

[0024] F-TATACTTTGTCTGAGTGGGGTATTC

[0025] R-AATTGTCCTCCGTTGGAGATGA

[0026] The PCR reaction program is: 95°C 5min→(94°C 45sec→54°C 45sec→72°C 60sec)×35 cycles→72°C 5min→4°C∞.

[0027] After the PCR reaction, take 3 μl of the PCR amplification product and perform electrophoresis on a double vertical plate denaturing polyacrylamide gel with a concentra...

Embodiment 2

[0029] Similar to Example 1, the difference is that the wrinkled pan abalone ♀ and western abalone are used Hybridization is carried out to obtain hybrid seedlings. Compare the electrophoresis results of the PCR amplification product with the provided standard spectrum. If only 1-2 bands appear in the range of 250-270bp when comparing the spectrum, it indicates that the DNA sample is Siberian abalone. If only 200-240bp If 1 or 2 bands appear in the interval, the DNA sample is abalone; if a band appears in the 250-270bp interval and 200-240bp interval in the sample at the same time, it means that it has 1 specific band of the parent at the same time. The individual is the first generation of hybrids of true Siberian abalone and wrinkled pan abalone, and the lack of any one of the bands is regarded as a false hybrid.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a DNA molecule identification method of coenospecies of Xishi abalone and Haliotis discus hannai Ino, relating to the DNA molecule marker detection of an abalone, in particular to a method for carrying out molecule identification to the authenticity of the coenospecies of an interspecific coenospecies generation of the Xishi abalone and the Haliotis discus hannai Ino by utilizing a molecule marker technology (microsatellite technology). The invention provides a DNA molecule identification method of the coenospecies of the Xishi abalone and the Haliotis discus hannai Ino by using microsatellite molecular marker, which has the advantages of high polymorphism, stable heredity and not being affected by environment conditions, etc, and can identify the authenticity of the coenospecies of the interspecific coenospecies generation of the Xishi abalone and the Haliotis discus hannai Ino during the large scale interspecific hybridization species production process of the Xishi abalone and the Haliotis discus hannai Ino. The genome DNA of the coenospecies generation of the Xishi abalone and the Haliotis discus hannai Ino is extracted by adopting a phenol chloroform method and PCR amplification reaction is carried out by taking the extracted genome DNA as a template to obtain the amplification product; gel electrophoresis and dyeing are carried out to the amplification product, and the map is compared; and comparison is carried out according to the electrophoresis result and the provided standard map.

Description

technical field [0001] The invention relates to the detection of DNA molecular markers of abalone, in particular to a method for molecular identification of the authenticity of the first generation of hybrids of the interspecies hybrids of Siberian abalone and wrinkled disc abalone using molecular marker technology-microsatellite (SSR) technology . Background technique [0002] Abalone is a kind of marine shellfish, known as the first of the eight seafood treasures, known as "soft gold", and has extremely high economic value. The main cultured abalone species in my country are Haliotis discus hannai and Haliotis diversicolor. Among them, Haliotis discus hannai is a temperate species and is mainly distributed in Liaodong Peninsula and Shandong Peninsula in my country. The area is the main cultured abalone species in the northern coastal areas of my country. Due to its high economic value, in recent years, industrialized artificial breeding of wrinkled plate abalone has been ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68
Inventor 骆轩柯才焕
Owner XIAMEN UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com