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Method for preparing bamboo leaf polysaccharide extract by member separation

A technology of bamboo leaf polysaccharides and extracts, which is applied in the field of membrane separation to prepare high-purity bamboo leaf polysaccharides, can solve the problems of low content of bamboo leaf polysaccharides, achieve the effects of increasing the content, benefiting protection and regeneration, and saving energy

Inactive Publication Date: 2011-04-20
INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the defect that the content of bamboo leaf polysaccharide in the bamboo leaf polysaccharide extract existing in the prior art is relatively low, the present invention provides a kind of method of membrane separation preparation bamboo leaf polysaccharide extract, can make polysaccharide content higher than 80%wt bamboo leaf polysaccharide extract

Method used

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  • Method for preparing bamboo leaf polysaccharide extract by member separation
  • Method for preparing bamboo leaf polysaccharide extract by member separation
  • Method for preparing bamboo leaf polysaccharide extract by member separation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Example 1 Screening method for high-content bamboo leaf polysaccharide

[0077] (1) Preparation of sulfuric acid-enone solution: Weigh 0.2 g of anthrone, dilute concentrated sulfuric acid in a 100 mL volumetric flask, mix well, and prepare immediately for use.

[0078] (2) Preparation of standard glucose solution: Accurately weigh anhydrous glucose dried to constant weight to prepare a solution with a concentration of 0.1200 mg / mL for later use.

[0079] (3) Glucose standard curve drawing: Accurately draw 0.0mL, 0.1mL, 0.2mL, 0.3mL, 0.4mL, 0.6mL, 0.8mL into a 10mL stoppered test tube, make up 1.0mL with distilled water, and use 1.0mL distilled water as a blank control , then add 4mL of sulfuric acid-enone solution, shake well, cool in ice water, then boil in boiling water for 10min, cool to room temperature rapidly, and measure the absorbance A at 620nm.

[0080] (4) Sample polysaccharide repeated determination test: take 4 parts of the same mass (2g) of any of 30 sample...

Embodiment 2

[0084] A method for preparing high-purity bamboo leaf polysaccharide by membrane separation, comprising the following steps:

[0085] In the first step, the bamboo leaf fine powder screened is extracted with a lipophilic solvent, wherein the mass ratio of the lipophilic solvent to the dried bamboo leaf fine powder is (1-30): 1, for example, the ratio can be selected as: 1: 1, 4:1, 7:1, 8:1, 10:1, 14:1, 17:1, 20:1, 22:1, 24:1, 27:1, 28:1, 29:1, 29.5:1, 30:1, the extraction temperature is 20°C-100°C, for example, it can be selected as: 25°C, 35°C, 40°C, 50°C, 60°C, 70°C, 75°C, 80°C, 90°C, 98°C ℃; extraction time is 0.5h-72h, extraction time is 0.5h, 1h, 3h, 5h, 6h, 8h, 9.5h, 12h, 14h, 15h, 16h, 17h, 18h, 20h, 30h, 35h, 40h, 55h , 60h, 70h, 71h.

[0086] In the second step, use C 1 -C 3 Fatty alcohol or acetone to extract the remaining bamboo leaf residue, the mass ratio of extractant to bamboo leaf is (1-20):1, for example, the ratio can be selected as: 1:1, 4:1, 7:1, 8:1, ...

Embodiment 3

[0095]Choose 20kg of 6-year-old fresh moso bamboo leaves collected in April in Youxi, Fujian, put them in a ventilated and cool place to dry, and then blow air in an oven below 50°C for 4hrs. The measured moisture content is lower than 7.0%, and the polysaccharide content is 4.72%. Take 8kg of dry leaves, crush them into fine powder, add 60kg of petroleum ether (60°C-90°C), extract under reflux at 70°C for 4 hours, add 40kg of petroleum ether (60°C-90°C) for the second extraction, and repeat the above operate. The extracts were combined, and the solvent was recovered under reduced pressure at 50°C. Add about 100L of industrial grade methanol to the residual leaves after petroleum ether extraction, set the temperature at 50°C, and extract overnight; add 50L methanol for the second extraction for 3 hours, at 70°C, filter, and combine the methanol extracts. After drying the residual leaves with direct steam, add 100L of deionized water, extract at 100°C for 2 hours, filter, repe...

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Abstract

The invention discloses a membrane separation method for preparing bamboo leaf polysaccharide extracts, which comprises the following steps: firstly, a lipophilic solvent is used for extracting screened bamboo leaves; secondly, aliphatic alcohol or acetone of C1-C3 is used for extracting the remained bamboo leaf slags; then, deionized water purified by a reverse osmose membrane is used for extracting remained bamboo leaf slags; thirdly, alkalic centrifugal settling is carried out to obtain a centrifugal clear solution and sediments; the centrifugal clear solution goes through the treatment of a ceramic membrane, an ultrafiltration membrane and a nanofiltration membrane sequentially, thus obtaining concentrated solution going through the nanofiltration membrane; the concentrated solution going through the nanofiltration membrane is adsorbed by a decolorizing resin; the polysaccharide solution is concentrated under vacuum until the ratio thereof is 1.03 to 1.14; the aliphatic alcohol of C1-C3 is used for settling the polysaccharide, and settlements are centrifuged and dried by microwave under vacuum; the solid part is dried by microwave under vacuum to obtain the bamboo leaf polysaccharide extracts with high-content polysaccharide.

Description

technical field [0001] The invention relates to a method for separating bamboo leaf polysaccharide, in particular to a method for preparing high-purity bamboo leaf polysaccharide through membrane separation. Background technique [0002] With the improvement of people's living standards, returning to nature, the concept of natural healthy food and green food has become more and more popular in people's life philosophy. Natural food and medicine have become the trend and fashion of international food and medicine development. Bamboo leaf extract is being recognized as a natural extract with various physiological functions. [0003] Bamboo is a class of plants belonging to the Gramineae Bambusadea (Bambusadea). There are about 70 genera and 1225 species in the world. Bamboo plants are distributed in tropical, subtropical and warm temperate regions between 46° north latitude and 47° south latitude on the earth, and are mainly distributed in tropical and subtropical regions of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 王成章陈文英叶建中陈虹霞周昊郑光耀
Owner INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY