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Separation and regeneration method of gulfweed protoplast

A technology of protoplasts and sargassum, applied to plant cells, etc., can solve problems such as the decline of natural resources, the intensification of the contradiction between supply and demand of sargassum, and the deterioration of the offshore environment, and achieve the effect of low cost, low cost of cultivation, and high regeneration survival rate

Inactive Publication Date: 2011-06-08
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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AI Technical Summary

Problems solved by technology

However, due to factors such as offshore harvesting, port construction, algae / fish / shellfish collection and breeding, the amount of natural resources of Sargassum is continuously declining, resulting in the increasing contradiction between supply and demand of Sargassum, and the offshore environment is becoming more and more serious. deterioration

Method used

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  • Separation and regeneration method of gulfweed protoplast

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Embodiment

[0033] 1. Preparation of enzyme solution

[0034] Preparation contains the enzyme liquid of mass concentration 1% cellulase, mass concentration 1% pectinase, 0.8mol / L conch enzyme, with artificial seawater (100mL distilled water contains 2.63gNaCl, 0.609g MgCl 2 , 0.193g MgSO 4 , 0.074g KCl, 0.11g CaCl 2 , 0.1g Tris (pH 8.3)) as the solvent, the above enzyme solution is the final concentration of the treatment, artificial seawater is prepared and sterilized under high pressure (0.12-0.14MPa) for 28 minutes.

[0035] 2. Sample collection and processing

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Abstract

The invention relates to the propagation of gulfweed, in particular to the separation and regeneration of the protoplast of the gulfweed. Enzyme liquid prepared by sterilized artificial seawater which comprises the cellulase with the weight concentration of 0.9 to 1.1 percent, pectase with the weight concentration of 0.9 to 1.1 percent and sea snail enzyme with weight concentration of 0.7 to 0.9 mol / L is used for carrying out zymohydrolysis to the sample of the guflweed for 5 to 7h under a dark condition at the temperature of 18 to 22 DEG C for centrifugating and obtaining the protoplast; andaccording to a normal method, the protoplast is paved into eutrophic seawater and cultured into a new gulfweed seedling. Based on the other plant researches of ancestors, the invention adds the sea snail enzyme and adopts simultaneously the cellulase, the sea snail enzyme and the pectase to remove the cytoderm of the gulfweed, successfully overcomes the problem of low yield of the protoplast, obtains a plurality of gulfweed protoplasts used for regeneration for the first time and successfully obtains a plurality of regenerated individuals of the protoplasts.

Description

technical field [0001] The invention relates to the breeding of Sargassum, in particular to the separation and regeneration of Sargassum protoplasts. Background technique [0002] The species of Sargassum spp, such as S.pallidum, S.muticum, S.thunbergii, S.polycystum, etc. Important economic seaweed, usually used as feed, alginate and raw material of pharmaceutical industry, can also be used as seaweed fertilizer, feed additive, etc.; Sargassum has always been the most important to maintain the ecological balance of my country's sea area because of its huge biomass and wide distribution. one of the macroalgae. In recent years, with the continuous deepening of people's research on the application of sargassum, more and more people have realized the economic value and ecological benefits of sargassum, the demand of enterprises for sargassum is increasing year by year, and the deteriorating environment also requires the restoration of original Some sargassum vegetation environm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04
Inventor 叶乃好王清印李晓川
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI