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Immunoglobulin G detection reagent

A technology of immunoglobulin and detection reagents, which is applied in the direction of biological testing, material inspection products, etc., can solve the problems of cumbersome operation and inability to analyze and detect batch samples, and achieve good repeatability, high accuracy, and strong anti-interference ability Effect

Inactive Publication Date: 2009-05-13
王贤理
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Known methods for measuring immunoglobulin G (IgG) include immunodiffusion method, immunoelectrophoresis method, and radioimmunoassay method. These methods are cumbersome to operate, require special equipment, and require pretreatment of samples. Batch sample analysis and analysis cannot be performed. Disadvantages such as not being able to be directly tested by a fully automatic biochemical analyzer

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0059] 1. IgG reagent (R1)

[0060] Phosphate (buffer) 100mmol / L

[0061] Polyoxyethylene lauryl ether (surfactant) 5%

[0062] NaCl (electrolyte) 20%

[0063] PEG-6000 (polymer accelerator) 4%

[0064] Broad-spectrum fungicide (preservative) 3%

[0065] Hexadimethylamine bromide (reaction accelerator) 0.3%

[0066] Disodium edetate (stabilizer) 0.5%

[0067] 2. Anti-IgG antibody reagent (R2)

[0068] Phosphate (buffer) 100mmol / L

[0069] Polyoxyethylene lauryl ether (surfactant) 5%

[0070] NaCl (electrolyte) 10%

[0071] PEG-6000 (polymer accelerator) 4%

[0072] Broad-spectrum fungicide (preservative) 0.4%

[0073] Disodium EDTA (stabilizer) 0.05%

[0074] Rabbit anti-human IgG antiserum 5%

[0075] Butylated hydroxyanisole (antioxidant) 0.01%

[0076] Mannitol (stabilizer) 5%

[0077] 3. Liquid serotype IgG constant value calibrator

[0078] CAPSO (buffer) 100mmol / L

[0079] Broad-spectrum fungicide (preservative) 0.06%

[0080] Disodium edetate (stabilize...

Embodiment 2

[0086] 1. IgG reagent (R1)

[0087] Phosphate buffer 5mmol / L

[0088] Polyoxyethylene lauryl ether (surfactant) 0.1%

[0089] NaCl (electrolyte) 0.1%

[0090] PEG-6000 (polymer accelerator) 20%

[0091] Broad-spectrum fungicide (preservative) 0.1%

[0092] Polybrene (reaction accelerator) 0.1%

[0093] Disodium edetate (stabilizer) 0.1%

[0094] 2. Anti-IgG antibody reagent (R2)

[0095] Phosphate (buffer) 10mmol / L

[0096] Polyoxyethylene lauryl ether (surfactant) 0.1%

[0097] NaCl (electrolyte) 0.1%

[0098] PEG-6000 (polymer accelerator) 7%

[0099] Broad-spectrum fungicide (preservative) 0.01%

[0100] Disodium EDTA (stabilizer) 2%

[0101] Goat anti-human IgG antiserum 40%

[0102] Butylated hydroxyanisole (antioxidant) 0.5%

[0103] Magnesium chloride (stabilizer) 1%

[0104] 3. Liquid serotype constant value IgG calibrator

[0105] CAPSO (buffer) 100mmol / L

[0106] Broad-spectrum fungicide (preservative) 0.01%

[0107] Disodium edetate (stabilizer) 0.1...

Embodiment 3

[0113] 1. IgG reagent (R1)

[0114] Phosphate buffer 200mmol / L

[0115] Polyoxyethylene lauryl ether (surfactant) 10%

[0116] NaCl (electrolyte) 40%

[0117]PEG-6000 (polymer accelerator) 80%

[0118] Broad-spectrum fungicide (preservative) 5%

[0119] Polybrene (reaction accelerator) 0.5%

[0120] Disodium edetate (stabilizer) 1%

[0121] 2. Anti-IgG antibody reagent (R2)

[0122] Phosphate (buffer) 200mmol / L

[0123] Polyoxyethylene lauryl ether (surfactant) 10%

[0124] NaCl (electrolyte) 5%

[0125] PEG-6000 (polymer accelerator) 2%

[0126] Broad-spectrum fungicide (preservative) 1%

[0127] Disodium EDTA (stabilizer) 0.05%

[0128] Horse anti-human IgG antiserum 20%

[0129] Butylated hydroxytoluene (antioxidant) 0.3%

[0130] Glycerol (stabilizer) 10%

[0131] 3. Liquid serotype constant value IgG calibrator

[0132] CAPSO (buffer) 20mmol / L

[0133] Broad-spectrum fungicide (preservative) 1%

[0134] Glycerol (stabilizer) 1%

[0135] Bovine serum albu...

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Abstract

The invention discloses a detecting reagent for immunoglobulin G, which comprises an IgG reactant, an anti-IgG antibody reagent and a liquid serological type constant-value calibrating agent, wherein, the IgG reactant can cause IgG antigen sites in a sample to be fully exposed so as to facilitate the full combination between the IgG antigens and the anti-IgG antibody reagent; the anti-IgG antibody reactant has high idiosyncrasy with IgG antigens in human serum; and the liquid serological type constant-value calibrating agent is used for sample comparison and result calculation. The anti-IgG antibody is from mammals of sheep, horses, mice, rabbits, or the like. The IgG reactant and the anti-IgG antibody reagent can be used as two independent reagents to form a double-reagent form of a product and also can be mixed according to certain proportions to form a single-reagent form of the product.

Description

technical field [0001] The invention relates to a reagent combination for measuring human body fluid components, in particular to a detection reagent for measuring immunoglobulin G (IgG) in human serum, which can be widely used in the technical fields of medicine and biochemistry. Background technique [0002] Immunoglobulins are a group of proteins with antibody activity, mainly found in plasma, but also in other body fluids, tissues and some secretions. Most of the immunoglobulins in human plasma exist in gamma globulin (γ-globulin). Can be divided into five classes, namely immunoglobulin G (IgG), immunoglobulin A (IgA), immunoglobulin M (IgM), immunoglobulin D (IgD) and immunoglobulin E (IgE). IgG is the most important immunoglobulin, accounting for about 70% of human plasma gamma globulin, with a molecular weight of about 150,000 and a sugar content of 2 to 3%. IgG molecules consist of 4 peptide chains. Among them, the peptide chain with a molecular weight of 25,000 i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53
Inventor 王贤理蒙凯蔡其浩
Owner 王贤理
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