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Ammonia ion diagnosis/measuring reagent kit and ammonia ion concentration determination method

A kit and ion technology, applied in the field of measuring ammonia concentration, ammonia diagnosis/determination kit, can solve the problems such as undiscovered blood ammonia measurement method

Inactive Publication Date: 2009-06-24
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Searching Chinese patents, only found 87105593.7 patent application which discloses a quick-frozen cup for measuring blood ammonia, but did not find an ideal method for measuring blood ammonia

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The ammonia (ion) diagnosis / measurement reagent of the present embodiment is a single reagent, comprising:

[0046] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0047] Stabilizer 500mmol / L

[0048] Reduced coenzyme 0.25mmol / L

[0049] Glutamine Synthetase 6000U / L

[0050] Phosphoenolpyruvate Carboxylase 8000U / L

[0051] Pyruvate dehydrogenase 10000U / L

[0052] Glutamic acid 10mmol / L

[0053] Adenosine triphosphate 3mmol / L

[0054] Oxaloacetate 5mmol / L

[0055] Acetyl-CoA 3mmol / L

[0056] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0057] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the ammonia (ion) sample to the reagent is 1 / 25, the reaction direct...

Embodiment 2

[0060] The ammonia (ion) diagnosis / measurement reagent of the present embodiment is a double reagent, comprising:

[0061] Reagent 1

[0062] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0063] Stabilizer 50mmol / L

[0064] Reduced coenzyme 0.25mmol / L

[0065] Glutamic acid 10mmol / L

[0066] Adenosine triphosphate 3mmol / L

[0067] Oxaloacetate 5mmol / L

[0068] Acetyl-CoA 3mmol / L

[0069] Reagent 2

[0070] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0071] Stabilizer 500mmol / L

[0072] Glutamine Synthetase 6000U / L

[0073] Phosphoenolpyruvate Carboxylase 8000U / L

[0074] Pyruvate dehydrogenase 10000U / L

[0075] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0076] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test subwavelength ...

Embodiment 3

[0079] The ammonia (ion) diagnosis / measurement reagent of the present embodiment is three reagents, comprising:

[0080] Reagent 1

[0081] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0082] Stabilizer 50mmol / L

[0083] Reduced coenzyme 0.25mmol / L

[0084] Glutamic acid 10mmol / L

[0085] Adenosine triphosphate 3mmol / L

[0086] Oxaloacetate 5mmol / L

[0087] Acetyl-CoA 3mmol / L

[0088] Reagent 2

[0089] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0090] Stabilizer 500mmol / L

[0091] Phosphoenolpyruvate Carboxylase 8000U / L

[0092] Pyruvate dehydrogenase 10000U / L

[0093] Reagent 3

[0094] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0095] Stabilizer 500mmol / L

[0096] Glutamine Synthetase 6000U / L

[0097] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0098] When measuring the ammonia (ion) conc...

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Abstract

The invention relates to a kit for diagnosing / measuring ammonia (ions) by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of ammonia (ions), and belongs to the technical field of medical / food / environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, glutamic acid, adenyl pyrophosphate, oxaloacetic acid, acetylcoenzyme A, glutamine synthetase, phosphoric acid enol pyruvate carboxylase, pyruvate dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the degree / velocity of the decrease in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the concentration of ammonia (ions).

Description

technical field [0001] The invention relates to an ammonia (ion) diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring ammonia (ion) concentration, which belongs to the technical field of medicine / food / environmental inspection and measurement. Background technique [0002] Ammonia determination methods include micro-diffusion method, ion exchange method, enzymatic method and ammonia electrode method. The most widely used methods are enzymatic method and blood ammonia analyzer analysis method based on ion selective electrode. [0003] The diffusion method is that after the sample is alkalized, NH is released 3 , Titrate the released ammonia with acid, or use the Nessler reaction to form brown-yellow mercuric ammonium iodide for colorimetry. These methods need to be alkalized, and the endogenous ammonia formation will affect the accuracy and precision, so they are rarely used at present; the ion exchange method is more accurate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N33/84C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD