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High-purity salvianolic acid B, preparation and use thereof

A salvianolic acid, high-purity technology, applied in the field of extraction and separation of botanical medicinal materials, can solve the problems of inability to use industrialized production, low purity of salvianolic acid B, and high cost, and achieves reduction of toxic side effects, low price, and low equipment investment. Effect

Inactive Publication Date: 2009-07-01
苑立超
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of the prior art is that the purity of salvianolic acid B obtained is lower than 92%, and there are many steps to obtain salvianolic acid B above 92%, and the cost is very high, which cannot be used for industrial production

Method used

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  • High-purity salvianolic acid B, preparation and use thereof

Examples

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Effect test

Embodiment 1

[0027] Take 1000g of Salvia miltiorrhiza, grind it, add deionized water, heat and extract at 100°C for 1 hour, use 5 times the weight of Salvia miltiorrhiza, and then extract twice at 50°C and pH 4.8, 40 each time Minutes, use 3 times the weight of salvia miltiorrhiza in water. Filter the extract, use D101 macroporous adsorption resin chromatography, wash with water to remove impurities, and then desorb with 40% ethanol to recover ethanol. Freeze-dry. After the obtained product is dissolved in ethanol, use cellulose powder chromatography column layer eluted with 95% ethanol to remove impurities, and then desorbed with water to obtain the composition containing salvianolic acid B, concentrated under reduced pressure, filtered through a mixed cellulose microporous membrane, and freeze-dried to obtain salvianolic acid with a purity of 99%. Acid B.

Embodiment 2

[0029] Take 1500g of Salvia miltiorrhiza, grind it, add deionized water, heat and extract at 100°C for 1 hour, use 5 times the amount of water, and then extract twice at 50°C and pH 4.8, 40 minutes each time, Use 3 times as much water. Filter the extract, use D1300 macroporous adsorption resin chromatography, wash with water to remove impurities, then desorb with 30% ethanol, recover ethanol, and freeze-dry. After the product is dissolved in ethanol, use cellulose powder chromatography column chromatography , eluted with 90% ethanol to remove impurities, then desorbed with water to obtain the composition containing salvianolic acid B, concentrated under reduced pressure, filtered through a mixed cellulose microporous membrane, and freeze-dried to obtain salvianolic acid with a purity of 98% b.

Embodiment 3

[0031] Take 2000g of Salvia miltiorrhiza, grind it, add deionized water, heat and extract at 100°C for 1 hour, use 5 times the amount of water, and then extract twice at 50°C and pH 4.8, 40 minutes each time, Use 3 times as much water. Filter the extract, use D1300 macroporous adsorption resin chromatography, wash with water to remove impurities, then desorb with 50% ethanol, recover ethanol under reduced pressure, and evaporate to dryness. After the product is dissolved in ethanol, use cellulose powder chromatography column Chromatography, eluted with 95% ethanol to remove impurities, and then desorbed with water to obtain the composition containing salvianolic acid B, concentrated under reduced pressure, filtered through a mixed cellulose microporous membrane, and freeze-dried to obtain salvianolic acid with a purity of 98.5%. Phenolic acid B.

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Abstract

The invention relates to high-purity danshinolic acid B and a preparation method and application thereof. The prior art has the problems that the purity of the danshinolic acid B obtained is less than 92 percent; acquisition of the danshinolic acid B with the purity more than 92 percent requires a plurality of steps; the cost is high; and the method can not be used for industrial production. The method comprises: a, performing hot-water extraction twice; b, filtering an extracting solution, using macroporous absorbent resin for chromatography, using water for elution, using 20 to 90 percent lower alcohol between C1 and C5 for desorption, decompressing and concentrating a stripping liquid, and obtaining a coarse product of the danshinolic acid B; c, using cellulose for further chromatography of the coarse product of the danshinolic acid B, using lower alcohol or acetone for elution, using water or the lower alcohol between C1 and C5 for desorption, merging compositions containing the danshinolic acid B, and obtaining the high-purity danshinolic acid B after vacuum concentration and freeze drying; and d, using a pH value regulating substance to adjust the pH value to between 1 and 6.5 in the steps a, b and c. The method is used for preparing the high-purity danshinolic acid B.

Description

Technical field: [0001] The invention relates to the category of extraction and separation of plant medicinal materials, in particular to a method for extracting and separating salvianolic acid B from Chinese medicinal material Salvia miltiorrhiza. Background technique: [0002] Salvia miltiorrhiza Bge, a plant of the Labiatae family, is a perennial herb that has the effects of dispelling blood stasis and relieving pain, promoting blood circulation and stimulating menstruation, and clearing heart and annoyance. In modern clinical practice, it is widely used to prevent and treat cardiovascular and cerebrovascular diseases, kidney diseases, liver diseases, etc. Due to the complex ingredients in the extract, it is difficult to promote it to the world. [0003] Salvianolic acid B is a water-soluble phenolic acid isolated from Salvia miltiorrhiza, and its molecular formula is C 36 h 30 o 16 , the molecular weight is 718.614, and the structural formula is: [0004] [0005...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/80A61K31/343A61P9/00A61P25/00A61P43/00A61K36/53
Inventor 苑立超
Owner 苑立超