Method for producing artemisinin by plant cell
A plant cell, artemisinin technology, applied in the production and fermentation of bulk chemicals, can solve the problems of wasting resources, laborious, and difficult to guarantee yield
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Embodiment 1
[0013] Example 1 Take dihydroartemisinic acid, dissolve it with absolute ethanol, add it to the cell suspension of Artemisia annua cultured for 14 days in a sterile ultra-clean workbench, co-cultivate for two days, terminate the reaction, filter with suction, and separate Cultures and media were obtained. The culture was rinsed with distilled water, dried at 55°C to constant weight, pulverized, ultrasonically extracted three times with eight times acetone, half an hour each time, and the extract was evaporated to dryness under reduced pressure to obtain extract I. The culture medium was concentrated to an appropriate amount, extracted with an equal amount of ethyl acetate, and the extract was evaporated to dryness under reduced pressure to obtain extract II. The two extracts were combined, separated by silica gel column chromatography, compared with TLC thin layer, combined with components containing artemisinin, and crystallized with petroleum ether to obtain pure artemisinin...
Embodiment 2
[0014] Example 2 Take artemisinic acid, dissolve it in methanol, and add it to the suspension culture medium of periwinkle cells in the logarithmic growth phase in a sterile ultra-clean bench, co-cultivate for two days, stop the reaction, filter with suction, and obtain culture and culture respectively. base. The culture was rinsed with distilled water, dried at 55°C to constant weight, pulverized, placed in a Soxhlet extractor, extracted three times with ten times ethyl acetate, half an hour each time, and the extract was evaporated to dryness under reduced pressure to obtain the extract I . The culture medium was concentrated to an appropriate amount, extracted with an equal amount of ethyl acetate, and the extract was evaporated to dryness under reduced pressure to obtain extract II. The two extracts were combined, separated by silica gel column chromatography, and compared with TLC thin layer, and the components containing artemisinin were combined and crystallized with e...
Embodiment 3
[0015] Example 3 Take 4,11-amorphadiene, dissolve it with absolute ethanol, add it to the tobacco suspension cell culture medium in the logarithmic growth phase in a sterile ultra-clean bench, co-cultivate for two days, terminate the reaction, and filter with suction , to obtain culture and medium, respectively. The culture was rinsed with distilled water, dried at 55°C to constant weight, pulverized, extracted three times with ethanol under reflux for half an hour each time, and the extract was evaporated to dryness under reduced pressure to obtain extract I. The culture medium was concentrated to an appropriate amount, extracted with an equal amount of ethyl acetate, and the extract was evaporated to dryness under reduced pressure to obtain extract II. The two extracts were combined, separated by silica gel column chromatography, and compared with TLC thin layer, and the components containing artemisinin were combined and crystallized with methanol to obtain pure artemisinin...
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