Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for preparing active anticancer fractions through conversion of total ginsenosides in panax plants by strain of fusarium sacchari

A technology of effective parts and total saponins, applied in the field of microorganisms, can solve the problems of occupying land and polluting the environment, and achieve the effects of reducing emissions, high tumor inhibition rate, and high product recovery rate.

Active Publication Date: 2013-02-13
SHENYANG PHARMA UNIVERSITY +1
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of collecting ginseng, the seeds and fruits are collected at the same time, the seeds are used to reproduce offspring, and the pulp is often thrown away as waste, which not only pollutes the environment but also occupies land

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing active anticancer fractions through conversion of total ginsenosides in panax plants by strain of fusarium sacchari
  • Method for preparing active anticancer fractions through conversion of total ginsenosides in panax plants by strain of fusarium sacchari
  • Method for preparing active anticancer fractions through conversion of total ginsenosides in panax plants by strain of fusarium sacchari

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Transformation of Panax notoginseng Stem and Leaf Saponins by Fusarium sugarcane

[0058] Inoculate Fusarium sugarcane cultured under optimal culture conditions into an aqueous solution containing 2g of Panax notoginseng saponins (content > 80%). -1 , Conversion (hydrolysis) under natural pH conditions. Sampling starts after 3d. The results of TLC showed that the polyglycoside Rb in Panax notoginseng stem and leaf saponins 1 , Rb 3 After 13 days, almost all the polyglycosides in the product were converted. After the bacteria were filtered, they were injected into a D101 macroporous adsorption resin column, washed with water until colorless, and then washed with ethanol gradient. The 80-90% ethanol eluate was concentrated and evaporated to dryness to obtain 1.18 g of the converted product.

Embodiment 2

[0060] Transformation of Ginseng Fruits by Fusarium sugarcane

[0061] Inoculate Fusarium sugarcane cultured under optimal culture conditions into an aqueous solution containing 2 g of ginseng fruit (content > 70%). -1 , Conversion (hydrolysis) under natural pH conditions. After 13 days of transformation, filter out the bacteria, inject it into a D101 macroporous adsorption resin column, wash it with water until it is colorless, and then wash the resin column with an ethanol gradient. The 80-90% ethanol eluate was concentrated and evaporated to dryness to obtain 1.04 g of the converted product.

Embodiment 3

[0063] Transformation of American Ginsenosides by Fusarium sugarcane

[0064]Inoculate Fusarium sugarcane cultured under optimal culture conditions into an aqueous solution containing 2g American ginseng saponin (content > 90%), transform at a temperature of 30°C, a shaker rotation speed of 160r min-1, and a natural pH value (hydrolysis). After 13 days of transformation, filter out the bacteria, inject it into a D101 macroporous adsorption resin column, wash it with water until it is colorless, and then wash the resin column with an ethanol gradient. The 80-90% ethanol eluate was concentrated and evaporated to dryness to obtain 1.14 g of the converted product.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a strain of fusarium sacchari and a method for preparing active fractions and ingredients with anticancer activity thereby and provides a method for screening the strain from soil for cultivating ginseng. A method for preparing ingredients with anticancer activity by the strain of fusarium sacchari is to prepare a large amount of active fractions with anticancer activity through the zymolysis of the total ginsenosides in panax plants by the fusarium saccharia, wherein the zymolysis temperature is 25 to 45 DEG C, the pH value is 5.0 to 7.0, the zymolysis time is 2 hours to 15 days, the conversion liquid is an aqueous solution, and the ratio of the total ginsenosides in the panax plants to the fusarium sacchari is 4:1-8:1; and a collection process comprises the steps of filtering reaction products to remove thalli, injecting the filtrate into a D101 large pore resin absorption column, washing filtrate with water till the filtrate is colorless, performing the gradient elution of the resin column with ethanol, concentrating 80 to 90 percent ethanol elution liquor till the ethanol elution liquor volatilizes completely, and obtaining the active fractions. The yield is 60 to 80 percent. The method is simple, convenient, safe, reliable and low in cost.

Description

Technical field: [0001] The invention relates to the technical field of microorganisms, and discloses a method for transforming (hydrolyzing) total saponins of plants of the genus Panax by microorganisms, and using the transformed total products as effective anticancer parts. In particular, it relates to a plant of Fusarium sacchari, a method for transforming total saponins of plants of the genus Panax to prepare anticancer effective parts and components. The composition of the effective part is mainly composed of ginsenosides C-K, Mx and Mc, the total content of the three saponins in the total transformation product is more than 60%, and the tumor inhibition rate reaches 70%. Background technique: [0002] Cancer is the second largest disease in the world that ravages human life, and its mortality rate is second only to cardiovascular and cerebrovascular diseases, and it is one of the most important factors of human death. Among the 6 billion people in the world, there are...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/258A61K36/424C12N1/20A61P35/00C12R1/01
Inventor 赵余庆韩颖姜彬慧胡筱敏
Owner SHENYANG PHARMA UNIVERSITY