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Production method for bioprotein feeding stuff

A technology of biological protein feed and production method, applied in the field of biological feed production, can solve the problems of unbalanced nutrient content, damage to human health, and low utilization rate, and achieve the effects of high utilization rate, balanced ratio and high nutrient content.

Inactive Publication Date: 2009-10-21
陈代文 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention aims to solve many technical problems such as shortage of traditional protein feed, extremely unbalanced nutrient content, low utilization rate, and damage to human health caused by the use of antibiotics in animal feed, so as to provide a low production cost, high output, Protein feed with high nutrient content and high utilization rate without adding antibiotics

Method used

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  • Production method for bioprotein feeding stuff
  • Production method for bioprotein feeding stuff
  • Production method for bioprotein feeding stuff

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] 1. Laboratory culture

[0054] 1. Primary culture of bacteria

[0055] 1) Prepare medium below 1L respectively

[0056] Lactobacillus medium (g)

[0057] Beef Extract 5.0

[0058] Yeast Extract 5.0

[0059] Glucose 5.0

[0060] Sodium acetate 5.0

[0061] Diamine citrate 2.0

[0062] Dipotassium hydrogen phosphate 2.0

[0063] Magnesium sulfate tetrahydrate 0.2

[0064] Manganese sulfate heptahydrate 0.05

[0065] Distilled water 1.0L

[0066] Yeast medium (g):

[0067]soybean sprouts 90

[0068] Glucose 50

[0069] Agar 15

[0070] Distilled water 1.0L

[0071] Bacillus culture medium (g):

[0072] Glucose 20

[0073] peptone 5

[0074] Sodium chloride 5

[0075] Beef Extract 0.5

[0076] Distilled water 1.0L

[0077] 2) Sterilization

[0078] After the above medium is prepared, it is divided into Erlenmeyer flasks, marked, and sterilized at 121°C-125°C for 20 minutes.

[0079] 3) Inoculation and cultivation

[0080] After cooling, aseptically in...

Embodiment 2

[0123] 1. Laboratory culture

[0124] 1. Primary culture of bacteria

[0125] 1) Prepare medium below 1L respectively

[0126] Lactobacillus medium (g)

[0127] Beef Extract 3.0

[0128] Yeast Extract 5.0

[0129] Glucose 3.0

[0130] Sodium acetate 4.0

[0131] Diamine citrate 1.0

[0132] Dipotassium hydrogen phosphate 1.0

[0133] Magnesium sulfate tetrahydrate 0.2

[0134] Manganese sulfate heptahydrate 0.05

[0135] Distilled water 1.0L

[0136] Yeast medium (g):

[0137] soybean sprouts 80

[0138] Glucose 30

[0139] Agar 10

[0140] Distilled water 1.0L

[0141] Bacillus culture medium (g):

[0142] Glucose 20

[0143] peptone 5

[0144] Sodium chloride 3

[0145] Beef Extract 0.5

[0146] Distilled water 1.0L

[0147] Following steps are with embodiment 1

Embodiment 3

[0149] 1. Laboratory culture

[0150] 1. Primary culture of bacteria

[0151] 1) Prepare medium below 1L respectively

[0152] Lactobacillus medium (g)

[0153] Beef Extract 4.0

[0154] Yeast Extract 60

[0155] Glucose 4.0

[0156] Sodium acetate 5.0

[0157] Diamine citrate 1.5

[0158] Dipotassium hydrogen phosphate 1.5

[0159] Magnesium sulfate tetrahydrate 0.3

[0160] Manganese sulfate heptahydrate 0.055

[0161] Distilled water 1.0L

[0162] Yeast medium (g):

[0163] soybean sprouts 85

[0164] Glucose 40

[0165] Agar 13

[0166] Distilled water 1.0L

[0167] Bacillus culture medium (g):

[0168] Glucose 25

[0169] peptone 6

[0170] Sodium chloride 4

[0171] Beef Extract 0.6

[0172] Distilled water 1.0L

[0173] Following steps are with embodiment 1

[0174] From the above, the production method of the biological protein feed of the present invention has many advantages such as low production cost, high output, high nutrient content, high util...

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Abstract

The invention provides a production method for bioprotein feeding stuff, which relates to the field of feeding stuff production, and aims to solve the technical problems of badly needed conventional protein feeding stuff, quite imbalanced nutrient contents, low utilization coefficient, harm to human health done by antibiotics used in the animal feeding stuff, and the like. The method comprises the following steps: respectively preparing a lactobacillus culture medium, a microzyme culture medium and a bacillus culture medium; respectively inoculating lactobacillus, microzyme and bacillus after germicidal treatment and cooling; saccharifying the three culture solutions, and introducing the culture solutions into a chemostat for chemostatic treatment; diluting the three bacteria solutions after the chemostatic treatment with water, mixing the three diluted solution with crushed solid feeding stuff, and carrying out inoculation, fermentation and low temperature drying on the mixture. The method is suitable for producing the protein feeding stuff.

Description

technical field [0001] The invention relates to the field of biological feed production, in particular to a production method of biological protein feed. Background technique [0002] According to relevant reports, most of the developing countries are agricultural countries, and the agricultural output value accounts for a large proportion of the economies of these countries. One of the effective ways to greatly increase the agricultural output value is to actively develop animal husbandry. The first problem to be solved in the development of animal husbandry is feed. The best way to solve the problem of feed is to plant it in large quantities. source of feed. Facing the pressure of huge population growth, the contradictions in demand for food, especially protein, are prominent. With the increasing pressure of population, resources and environment, these processing industries are facing huge challenges. The protein feed used as feed is even more in short supply, and countr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00A23K1/16C12N1/20A23K10/12
Inventor 陈代文余冰张克英徐静丁雪梅何军
Owner 陈代文