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Electrochemical gene sensor probe and preparation method thereof

A sensor probe, electrochemical technology, applied in the direction of biochemical equipment and methods, scientific instruments, instruments, etc., can solve the problems of poor orientation of probe matrix electrodes, low probe coverage, small probe binding force, etc., to achieve a unique Electronic properties, increased sensitivity, improved effective surface effects

Inactive Publication Date: 2009-11-04
HARBIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention aims to solve the problem of low probe coverage and low probe activity (resulting in poor sensitivity) in current gene sensors, as well as small and unstable probe binding force, incapable of quantitative analysis, short service life, and poor orientation on probe substrate electrodes. defect; and provide a kind of electrochemical gene sensor probe and preparation method thereof

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  • Electrochemical gene sensor probe and preparation method thereof

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specific Embodiment approach 1

[0013] Specific Embodiment 1: In this embodiment, the electrochemical gene sensor probe is made of glassy carbon electrodes, platinum nanoparticles, avidin and biotin-labeled ssDNA probes; platinum nanoparticles are deposited on the surface of the glassy carbon electrodes, avidin is adsorbed On deposited platinum nanoparticles, avidin binds biotin-labeled ssDNA probes via affinity interaction.

[0014] In this embodiment, complementary DNA strands can be designed as ssDNA probes according to the sequences of different target genes (detection target genes).

[0015] In this embodiment, the combination of nanoparticles and avidin sets a precedent in the technical route of modifying electrodes.

specific Embodiment approach 2

[0016] Specific embodiment two: In this embodiment, the electrochemical gene sensor probe is prepared according to the following steps: 1. Al with a diameter of 0.01 μm 2 o 3 Powder polish the glassy carbon electrode to the mirror surface, then ultrasonically clean it, and then add H at a concentration of 0.5mol / L 2 SO 4 Scan the cyclic voltammetry in the solution until a stable voltammogram is obtained, then put the glassy carbon electrode into the mixed solution and scan until the peak potential difference of the cyclic voltammetry curve is ≤70mV; 2. Immerse the glassy carbon electrode treated in step 1 in H 2 SO 4 The concentration is 0.5mol / L, K 2 PtCl 6 In the electrodeposition solution with a concentration of 2.5mmol / L, platinum nanoparticles were electrodeposited by the Step Functions method; three, avidin was adsorbed on the platinum nanoparticles; four, biotin-labeled ssDNA with a concentration of 1OD / mL was detected Add the needle dropwise to the platinum nano-d...

specific Embodiment approach 3

[0020] Specific embodiment 3: The difference between this embodiment and specific embodiment 2 is: in step 1, ultrasonic cleaning is performed twice, and each ultrasonic cleaning is 2 minutes. Other steps and parameters are the same as those in Embodiment 2.

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Abstract

The invention relates to an electrochemical gene sensor probe and a preparation method thereof. The invention solves the defects of low sensitivity, low probe bonding force, short service life, poor orientation of probe on a substrate electrode, and the like of the existing gene sensors. The probe of the invention is made of a glassy carbon electrode, platinum nanoparticles, avidin and a bioti-labeled ssDNA probe. The preparation method is as follows: first, pretreating the glassy carbon electrode; second, electrodepositing the platinum nanoparticles; third, absorbing the avidin on the platinum nanoparticles; and fourth, dropwise adding the bioti-labeled ssDNA probes to the platinum nano-deposit electrode on which the avidin is adsorbed, and cleaning; and finally, the electrochemical gene sensor probe can be obtained. The probe of the invention has the advantages of high sensitivity, high and stable probe bonding force, long service life and favorable orientation of probe on the substrate electrode.

Description

technical field [0001] The invention relates to a gene sensor probe and a preparation method thereof. Background technique [0002] Genetically Modified Food (GMF) refers to food, food raw materials and food additives made or produced by genetically modified organisms, and mainly refers to genetically modified plants used as food. Genetically modified organisms and genetically modified foods have been developed at a rapid pace since they were discovered in the 1970s. However, compared with traditional natural food, genetically modified food is still a new thing, there are uncertain factors and unknown effects, and its safety has been questioned. [0003] In order to evaluate the safety of genetically modified food, it is necessary to test the genetically modified ingredients. The use of PCR technology to detect exogenous genes has defects such as expensive equipment, high operating conditions, and the need to combine with electrophoresis technology, making it difficult to ...

Claims

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Application Information

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IPC IPC(8): G01N27/327C12Q1/68
Inventor 杜晓燕王茂清
Owner HARBIN MEDICAL UNIVERSITY