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Human anti human interferon alpha antibody and application thereof

A technology of antibodies and amino acids, applied in the fields of application, antibodies, anti-cytokine/lymphokine/interferon immunoglobulin, etc., can solve the problem that the pathogenesis is not very clear

Inactive Publication Date: 2011-09-14
STATION OF VIRUS PREVENTION & CONTROL CHINA DISEASES PREVENTION & CONTROL CENT +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its pathogenesis is still unclear

Method used

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  • Human anti human interferon alpha antibody and application thereof
  • Human anti human interferon alpha antibody and application thereof
  • Human anti human interferon alpha antibody and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0042] This example uses phage surface display technology to construct a human-derived fully synthetic genetically engineered antibody library. A fully synthetic phage antibody library was screened for enrichment with purified huIFN-α1b and secreted in E.coli. The functional activity of the scFv antibody specifically binding to huIFN-α1b was identified by ELISA, Western Blot and Pμll Down, and the sequence was determined. Then, the light chain and heavy chain variable region genes of the positive clones were respectively cloned into the whole antibody expression vector pAC-K-CH3 to transfect insect Sf9 cells, and the secreted expression of the whole antibody was realized by using the baculovirus / insect cell system. ELISA and Western Blot were used to identify the immunological characteristics and immune regulation function of the obtained human monoclonal antibody.

[0043] Materials and Methods

[0044] 1. Preparation of Cells, Vectors, and Antigens

[0045] The fully synt...

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Abstract

The invention provides a human anti huIFN-alpha antibody. In the invention, genetic engineering is combined with phage display technology to screen out a genetic engineering monoclonal antibody against huIFN-alpha from human fully synthetic single chain antibody library and obtain the antibody genes, thus finishing the identification of immunocompetence and biological activity of antibody proteinand providing new specific antibody medicines for clinical treatment of systemic lupus erythematosus.

Description

technical field [0001] The present invention relates to the preparation and application of human genetically engineered antibodies for treatment, especially human interferon-α (huIFN-α), which is specific for the therapeutic target protein of Systemic lupus erythematosus (SLE), and has the ability to neutralize the blood of lupus patients. Genetically engineered antibodies for neutralizing systemic diseases caused by elevated huIFN-α. Background technique [0002] Antibody drugs developed by using the blocking effect of antibody molecules are new forces developed in the field of biomedicine in recent years. According to statistics, since the birth of the first genetically engineered antibody human-mouse chimeric antibody OKT3 in 1984, there are currently about 20 kinds of genetically engineered antibody drugs approved by the FDA, and another 150 kinds are in the clinical research stage. By 2008, the revenue of antibody drugs will account for 30% of the total revenue of biot...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/13C12N15/63C12N1/19C12N1/21C12N5/10C07K16/24G01N33/577A61P37/02A61K39/395
CPCC07K2317/622C07K16/249C07K2317/24A61P37/02
Inventor 梁米芳孙志伟王颖龚斌王双李金枝李川孙丽娜李德新
Owner STATION OF VIRUS PREVENTION & CONTROL CHINA DISEASES PREVENTION & CONTROL CENT