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Method for sieving Epsilon-polylysine producing strains

A poly-lysine, bacteria-producing technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as long cycle

Inactive Publication Date: 2009-11-18
SHANGHAI INST OF PHARMA IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this method still has many deficiencies, and melanin has a great inhibitory effect on ε-polylysine-producing bacteria, and the cycle is long

Method used

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  • Method for sieving Epsilon-polylysine producing strains
  • Method for sieving Epsilon-polylysine producing strains
  • Method for sieving Epsilon-polylysine producing strains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] (1) Screening of strains producing positively charged substances

[0076] Add 10ml of sterilized distilled water to 1g of soil sample, perform a series of dilutions after filtration. Sterilize the SG solid medium at 121°C for 20 minutes, and add neutral red solution (final concentration 0.0015%)+K when the temperature drops to about 60°C 2 Cr 2 o 7 (final concentration 50mg / ml), make a plate after mixing.

[0077] Spread the diluted soil samples onto the above-mentioned plates and incubate at 30°C. After 4 days of culture, since neutral red can form a unique transparent circle on the plate with the positively charged polymer secreted by microorganisms, a transparent circle is formed around the strains that can produce positively charged substances, while the strains that do not produce positively charged substances There is no such phenomenon, so the strains producing positively charged substances can be screened out very sensitively.

[0078] (2) Determination of ...

Embodiment 2

[0084] (1) Screening of strains producing positively charged substances

[0085] Add 10ml of sterilized distilled water to 1g of soil sample, perform a series of dilutions after filtration. Sterilize the SG solid medium at 121°C for 20 minutes, and add amaranth solution (final concentration 0.005%)+K when the temperature drops to about 60°C 2 Cr 2 o 7 (final concentration 50mg / ml), make a plate after mixing.

[0086] Spread the diluted soil samples onto the above-mentioned plates and incubate at 30°C. After 4 days of culture, because amaranth can form a unique aggregation circle on the plate with the positively charged polymer secreted by microorganisms, a clustering circle is formed around the strain that can produce positively charged substances, while the strain that does not produce positively charged substances does not. This phenomenon, therefore, can very sensitively screen out the strains that produce positively charged substances.

[0087] (2) Determination of al...

Embodiment 3

[0093] (1) Screening of strains producing positively charged substances

[0094] Add 10ml of sterilized distilled water to 1g of soil sample, perform a series of dilutions after filtration. Sterilize the SG solid medium at 121°C for 20 minutes, and add allura red solution (final concentration 0.005%)+K when the temperature drops to about 60°C 2 Cr 2 o 7 (final concentration 50mg / ml), make a plate after mixing.

[0095] Spread the diluted soil samples onto the above-mentioned plates and incubate at 30°C. After 4 days of culture, because allura red can form a unique aggregation circle on the plate with the positively charged polymer secreted by microorganisms, a clustering circle is formed around the strains that can produce positively charged substances, while the strains that do not produce positively charged substances do not. This phenomenon, therefore, can very sensitively screen out the strains that produce positively charged substances.

[0096] (2) Determination of ...

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Abstract

The invention provides a method for sieving Epsilon-polylysine producing strains, which comprises the step of adding indicator with charges into culture medium, and the indicator with charges is not methylene blue. The sieving method can effectively visually separate the Epsilon-polylysine producing strains from other microorganisms, and has simple process, small workload, short period and higher sieving efficiency.

Description

technical field [0001] The invention belongs to the field of microbial fermentation engineering, in particular, the invention relates to a screening method for ε-polylysine producing bacteria. Background technique [0002] With the improvement of people's living standards and the gradual improvement of food safety awareness and requirements, chemical preservatives are facing severe challenges, and the naturalization of food anti-mold preservatives has become a future development trend. Natural food preservatives can be divided into microbial source preservatives, animal source and plant source preservatives. Microbial food preservatives refer to substances with antibacterial effect that are extracted and separated from fermentation broth through microbial fermentation. At present, only nisin, natamycin and ε-polylysine are the only microbial food preservatives approved internationally. Nisin can effectively inhibit many Gram-positive bacteria that cause food spoilage, but ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
Inventor 孙湘婷王岩陈少欣
Owner SHANGHAI INST OF PHARMA IND CO LTD