High-purity salvianolic acid B and preparation method and application thereof
A high-purity salvianolic acid technology, applied in the fields of high-purity salvianolic acid B, preparation and application, can solve the problems of inability to use industrialized production, low purity of salvianolic acid B, many steps, etc. Cost, simple process effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0024] Take 1000g of Salvia miltiorrhiza, grind it, add deionized water, heat and extract at 100°C for 1 hour, use water 5 times the weight of Salvia miltiorrhiza, and decoct and extract twice under the condition of pH 4.8, 40 minutes each time, use the weight of Danshen 3 times water. Filter the extract, adjust the pH to 3, use polyamide pre-column chromatography, wash with water to remove impurities, then use 10% ethanol to remove impurities, and 80% ethanol for desorption. The components containing salvianolic acid B were collected and recovered under reduced pressure. The resulting solution was then chromatographed on a polyamide column and desorbed with 95% ethanol to obtain components containing salvianolic acid B. The fractions with a purity greater than 98.5% were collected in sections, concentrated under reduced pressure, and adjusted to pH 4.8. The concentrated solution is passed through a Sephadex column, and the parts with a purity greater than 98.5% are collected...
Embodiment 2
[0026] Take 1500g of Salvia miltiorrhiza, grind it, add deionized water, heat and extract at 100°C for 1 hour, use 5 times of water, then decoct and extract 3 times under the condition of pH4.8, each time for 40 minutes, use 3 times of water . The extract was filtered, the pH was adjusted to 2, and the polyamide pre-column chromatography was used to elute impurities with water, and then 30% ethanol was used to remove impurities, and 90% ethanol was used for desorption. The components containing salvianolic acid B were collected and recovered under reduced pressure. The resulting solution was then chromatographed on a polyamide column and desorbed with 90% ethanol to obtain components containing salvianolic acid B. The fractions with a purity greater than 98% were collected in sections, concentrated under reduced pressure, and adjusted to pH 5. The concentrated solution is passed through a Sephadex column, and the parts with a purity greater than 98.5% are collected in section...
Embodiment 3
[0028] Take 2000g of Salvia miltiorrhiza, grind it, add deionized water, heat and extract at 100°C for 1 hour, use 5 times the water, and then decoct and extract twice under the condition of pH4.8, each time for 40 minutes, use 3 times of water. Filter the extract, adjust the pH to 4, use polyamide pre-column chromatography, wash with water to remove impurities, then use 50% ethanol to remove impurities, use 95% ethanol to desorb, and collect the components containing salvianolic acid B. Ethanol was recovered under reduced pressure. The resulting product was then chromatographed on a polyamide column, desorbed with 95% ethanol, and the fractions with a purity greater than 98% were collected in sections, concentrated under reduced pressure, and adjusted to pH 4.8. The concentrated solution is passed through a Sephadex column, and the parts with a purity greater than 98.5% are collected in sections, concentrated under reduced pressure, and freeze-dried to obtain high-purity salv...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More