Ganoderma strain capable of producing laccase

Abstract

The invention provides a ganoderma strain capable of producing laccase and the ganoderma strain is Ganoderma weberianum TZC1 with a preservation number CGMCCNo. 2648 of China General Microbiological Culture Collection Cente. The Ganoderma weberianum TZC1 of the invention can produce laccase in fermentation process and the activity of the laccase can reach 8620,830 U / L, which is 253 times of the highest enzymatic activity in the prior art. The laccase secreted by the Ganoderma weberianum TZC1 of the invention can keep better activity within a wide range of temperature and pH value, can promotethe decomposition of lignin and has significant promoting effect for decolorizing the dye for printing and dyeing.

Description

technical field [0001] The invention relates to the field of biochemistry, in particular to a fungal strain for fermenting laccase. Background technique [0002] Laccase (Laccase, EC1.10.3.2) is a class of copper-containing polyphenol oxidases, mainly including plant laccases and fungal laccases; the former mainly catalyzes the oxidative polymerization of substrates and is related to plant wound protection [Raifer Hou et al. (2003) Chinese lacquer, 2003 (1): 4-7]; the latter is mainly found in wood-rot fungi, which can promote the degradation of substrates such as lignin [Bajpai (1999) Biotech Prog, 15: 147-157 ; Tu Ningyu et al. (2006) Paper Science and Technology, 25: 27-31]. Fungal laccases have a wide range of uses. In wood processing, laccase is used to promote surface gluing, which can replace glue; in paper industry, laccase is used to bleach paper, reduce pulp hardness, reduce chemical consumption and energy consumption, and reduce organic chlorine content in waste...

AI Technical Summary

Problems solved by technology

In the past ten years, especially in the past five or six years, the research in this area has made some progress, but there are still many problems: most of the test strains have a long fermentation period when growing on artificial and natural medium, are not easy to operate, and produce The enzyme ability is weak, and often requires toxic or expensive inducers, which is not environmentally friendly, resulting in high production costs using existing strains, which limits the industrialization process of laccase

Although the above-mentioned method utilizes the output of Ganoderma lucidum fermentation laccase to have greater improvement, there is still a considerable distance from industrialized production.

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