Azithromycin nanostructured lipid carrier and preparation method thereof
A technology of nanostructured lipids and azithromycin, applied in the field of carrier systems, can solve problems such as the limitation of ophthalmic diseases, and achieve the effect of simple and convenient preparation process, good stability and high repeatability
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Embodiment 1
[0040] Example 1 Preparation of Azithromycin Nanostructured Lipid Carrier
[0041] Composition formula:
[0042]
[0043] Preparation:
[0044] 1. Weigh 21 grams of polyoxyl stearate S-40, 9 grams of poloxamer F-68, 2.5 grams of glyceryl monostearate and 2.5 grams of castor oil, and put them into the sample tube;
[0045] Heated in a water bath at 2.75°C to make it completely melted, then added 0.5 g of azithromycin and stirred magnetically to obtain a clarified system;
[0046] 3. Add 65 ml of distilled water at 75°C to the above clarification system;
[0047] 4. Stir for 15 minutes;
[0048] 5. Perform 3 cycles of high-pressure homogenization of the above-mentioned colostrum at 800 bar;
[0049] 6. Naturally cool to room temperature to obtain azithromycin lipid nanoparticle dispersion.
[0050] The average particle diameter after preparation is 74.5 nanometers, and the average particle diameter after storage for 0.5 months is 75.2 nanometers
Embodiment 2
[0051] Example 2 Preparation of Azithromycin Nanostructured Lipid Carrier
[0052] Composition formula:
[0053]
[0054] Preparation:
[0055] 1. Weigh 16.8 grams of polyoxyl stearate S-40, 7.2 grams of poloxamer F-68, 2 grams of glyceryl monostearate, and 2 grams of glyceryl triacetate, and put them into the sample tube;
[0056] 2. Heating in a water bath at 70°C to make it completely melted and then adding 0.4 g of azithromycin to obtain a clarified system;
[0057]3. Add 62 ml of distilled water at 60°C to the above clarification system;
[0058] 4. Stir for 10 minutes;
[0059] 5. Carry out 4 cycles of high-pressure homogenization of the above-mentioned colostrum at 700 bar.
[0060] 6. Cool naturally to room temperature to obtain the azithromycin lipid nanoparticle dispersion.
Embodiment 3
[0061] Example 3 Preparation of Azithromycin Nanostructured Lipid Carrier
[0062] Composition formula:
[0063]
[0064]
[0065] Preparation:
[0066] 1. Weigh 12.6 grams of polyoxyl stearate S-40, 5.4 grams of poloxamer F-68, 1.5 grams of glyceryl monostearate, and 1.5 grams of castor oil, and put them into the sample tube;
[0067] 2. Heating in a water bath at 70°C to make it completely melted and then adding 0.3 g of azithromycin to obtain a clarified system;
[0068] 3. Add 79 ml of distilled water at 60°C to the above clarification system;
[0069] 4. Stir for 10 minutes;
[0070] 5. Carry out 4 cycles of high-pressure homogenization of the above-mentioned colostrum at 600 bar.
[0071] 6. Cool naturally to room temperature to obtain the azithromycin lipid nanoparticle dispersion.
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