Cell culture method using amino acid-enriched medium
A culture method and technology for culturing cells, applied in cell culture medium, genetically modified cells, and cells modified by introducing foreign genetic material, etc., can solve the problems of culture deviation, unclear effects of other amino acids, and insufficient protein production. , to achieve the effect of small batch-to-batch variation, conducive to mass supply, and clear composition of ingredients
Inactive Publication Date: 2010-03-03
CHUGAI PHARMA CO LTD
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Problems solved by technology
The use of these substances has problems such as the use of expensive products, including biologically-derived components even in trace amounts, and variations in culture due to batch differences.
However, since these knowledges were studied only in CHO cells expressing glutamine synthetase, they were not verified in normal CHO cells, and the respective effects of other amino acids were also unclear.
In addition, protein production can not be said to be sufficient
Method used
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It is intended to provide a novel culture method for producing a desired protein by culturing cells capable of producing this protein with the use of a medium in which biological medium components areminimized. Namely, a culture method characterized by comprising conducting the culture while maintaining the concentration of a specific amino acid in the liquid culture medium at a high level, and afeeding culture medium for cell culture that is to be used in this method.
Description
technical field [0001] The present invention relates to a method for producing a desired protein by culturing cells producing it and a medium used in the method. In detail, the present invention relates to a serum-free culture method and protein production method, characterized in that in the method of producing the desired protein by culturing cells producing the desired protein, by maintaining the serine in the culture medium at a high Concentrations for culturing, and fed-batch medium for cell culture for use in the method is also provided. Background technique [0002] When culturing animal cells to obtain a natural protein produced by the animal cells, or to produce a desired protein by culturing animal cells into which a gene encoding a desired protein has been introduced, other than salts, sugars, and amino acids In addition to basic nutrients such as vitamins, mammalian-derived extracts, specifically serum such as fetal bovine serum, have been added to the culture m...
Claims
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IPC IPC(8): C12N5/10A61K38/00C12N5/06C12N15/09C12N15/13C12P21/00C12P21/02C12P21/08C12N5/07C12N5/071
CPCC07K16/2866C12N2500/33C12N2500/32C12P21/02C12N5/0018C07K16/303C12N2510/02C07K2317/24A61P37/04C12N5/00C12N15/11C12N5/10C12P21/00
Inventor 片山聪岸下升平小平邦彦定光信高木良智松田广记
Owner CHUGAI PHARMA CO LTD