Method for cultivating and propagating epipactis mairei

Abstract

The invention provides a method for cultivating and propagating epipactis mairei. The method is characterized in that: michelia crassipes law is used as a female parent, michelia calcicola is used as a male parent, flowers of the male parent in a bloom stage are picked, perianth lobes and gynoecia are removed, and pollen is collected when anthers naturally cracks to give off the pollen; flowers to be bloomed of the mother parent are picked, perianth lobes and gynoecia are removed, and the flowers are pollinated with the fresh pollen of the male parent; the pollinated flowers are bagged and bags are removed after one week; when cracking to show fresh red seeds, legumes and follicles are picked, aril is removed, the legumes and follicles are humidified and preserved in sand in a green house in winter and taken out in March or April in the following year to be sown by a conventional method, after sewing, the cross seedlings of F1 generation are obtained and cultured; and from the plants of the F1 generation, plants are selected to be cultured by a tissue culture method, wherein the selected plants have the characteristics that: the bus, flower stalks, twigs and leaf stalks are densely covered with red brown villi and stipule scars are in a length which is three fifths of that of the leaf stalks; the flowers have 7 to 9 light yellow perianth lobes; and the root parts of the inside surfaces have purple veins; stamens are purple and have 2 to 3 purple red petals; the plants with green gynoecia and purple red column heads are propagated by the tissue culture method.

Description

[0001] Field: [0002] The invention relates to the field of biotechnology, in particular to a method for cultivating a hybrid Michelia japonica from the F1 generation of hybrids of Michelia limestone and Michelia zihua, and a method for tissue culture and propagation of Michelia japonica. Background technique: [0003] Michelia L. is an important plant group in the ancient Magnoliaceae (Magnoliaceae), which are evergreen trees or shrubs. Most species have luxuriant branches and leaves, beautiful plant shapes, beautiful flowers and fragrant flowers, and are excellent garden ornamental tree species. [0004] Main features of M.calcicola: Buds are densely covered with yellow-brown long hairs; flowers are yellow, with 9 tepals, 4.0-4.5cm long, 1.0-1.5cm wide; stamens are yellow-brown, filaments are yellow-green, anthers are yellow Brown; pistil group green, stigma brown. [0005] Main characteristics of M. crassipes: Buds are densely covered with reddish-brown or yellowish-brown...

AI Technical Summary

Problems solved by technology

There is no report on the tissue culture propagation method of Yuxin Michelle

Images