Unlock instant, AI-driven research and patent intelligence for your innovation.

Tissue culture and rapid propagation method of raspberries

A tissue culture and red raspberry technology, applied in the field of plant cultivation, can solve the problems of difficulty in large-scale, standardized production of good varieties, large-scale promotion, slow reproduction speed and high cost, and achieves the improvement of transplanting survival rate, increasing proliferation rate, The effect of reducing variation

Active Publication Date: 2010-04-21
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
View PDF0 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the propagation speed of the above-mentioned propagation method is slow, the requirement for the female parent material is high, the dosage is large, and it is also limited by the season, so it is difficult to carry out large-scale, standardized production and large-scale promotion of fine varieties.
In addition, the current method of using tissue culture to reproduce and produce seedlings has high cost and long culture period.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1. Sterilization of explants: Collect the young and tender branches of red raspberry, cut them into 2cm-long sections with buds, wash them with washing powder water, and use alcohol with a volume concentration of 75% in a sterile ultra-clean bench to rub them on the surface Disinfect for 30 seconds, then use HgCl with a mass concentration of 0.1% 2 Sterilize for 6 minutes, then sterilize in a sodium hypochlorite solution with a volume concentration of 2% for 15 minutes, and finally rinse with sterile water for 3 times;

[0048] 2. Adventitious bud induction culture: inoculate the above-mentioned explants sterilized by the above-mentioned step 1 into the following induction medium in a sterile working environment: MS medium + BA 1.0mg / L + NAA 0.1mg / L + sugar 30g / L+agar 5g / L, pH 5.8, cultured for 18 days under the conditions of light intensity of 1500lx, culture temperature of 20°C, and photoperiod of 8h / d, 35% of the explants were free from pollution and grew out Leaf b...

Embodiment 2

[0053] 1. Sterilization of explants: Collect the young and tender branches of red raspberry, cut them into 3cm-long sections with buds, wash them with washing powder water, and use alcohol with a volume concentration of 70% in a sterile ultra-clean bench to clean the surface Disinfect for 60 seconds, then use HgCl with a mass concentration of 0.1% 2 Sterilize for 10 minutes, then sterilize in a sodium hypochlorite solution with a volume concentration of 1.5% for 20 minutes, and finally rinse twice with sterile water;

[0054] 2. Adventitious bud induction culture: inoculate the above-mentioned explants sterilized in the above step 1 into the following induction medium in a sterile working environment: MS medium + BA 0.5mg / L + NAA 0.3mg / L + sugar 40g / L+agar 7g / L, pH 6.0, cultured for 15 days under the conditions of light intensity of 2000lx, culture temperature of 27°C, and photoperiod of 10h / d, 30% of the explants were free from pollution and grew out Leaf bud clusters, cut o...

Embodiment 3

[0059] 1. Sterilization of explants: Collect the young and tender branches of red raspberry, cut them into 2cm-long sections with buds, wash them with washing powder water, and use alcohol with a volume concentration of 70% in a sterile ultra-clean bench to clean the surface Disinfect for 45 seconds, then use HgCl with a mass concentration of 0.15% 2 Sterilize for 8 minutes, then sterilize in a sodium hypochlorite solution with a volume concentration of 2% for 18 minutes, and finally rinse with sterile water for 3 times;

[0060] 2. Adventitious bud induction culture: Inoculate the above-mentioned explants sterilized in the above-mentioned step 1 into the following induction medium in a sterile working environment: MS medium + BA 1.5mg / L + NAA 0.2mg / L + sugar 35g / L+agar 6g / L, pH 5.8, cultured for 10 days under the conditions of light intensity of 1800lx, culture temperature of 25°C, and photoperiod of 9h / d, 25% of the explants were free from pollution and grew out Leaf bud cl...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a tissue culture and rapid propagation method of raspberries. The method comprises the following steps: using the stem tips of raspberries or stem sections with buds as explants; directly inducing and culturing in an induction culture medium to generate bud bushes; then, alternately propagating and culturing a needed quantity of bud bushes in a propagation culture medium; and after rooting culture, directly putting in seedling bags for transplantation to obtain raspberry seedlings. The method is high-efficiency and rapid, is needless to collect a great quantity of explants to obtain sterile seedlings, achieves the aims of improving propagation rate and reducing variation by adjusting hormones at various culture stages and mixing and alternately using the hormones, eliminates a link for transplanting grouped culture seedling nutrition pans, improves the transplantation survival rate, facilitates the transmission of survival seedlings, directly plants after picking off the seedling bags in plantation and fully realizes the scaled and standardized production of fine-variety raspberry seedlings.

Description

technical field [0001] The invention relates to a plant tissue culture and propagation method, in particular to a red raspberry tissue culture and rapid propagation method, belonging to the technical field of plant cultivation. Background technique [0002] Red raspberry belongs to Rosaceae, Rubus is a shrub fruit tree, the height of the plant is 1-2m, the berries are larger, and the fruit is red or orange. Red raspberry fruit is rich in nutrition, soft and juicy, unique in fragrance and bright in color, suitable for processing into high-end products such as fruit juice, jam and natural pigments. The conventional propagation methods of red raspberry include root division method, division method, cutting method and layering method. However, the propagation speed of the above-mentioned propagation method is slow, the requirement for the female parent material is high, the dosage is large, and it is also limited by the season, so it is difficult to carry out large-scale, stand...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 张倩杨春梅赵培飞汪国鲜吴丽芳
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI