Method for culturing microbial strain for degrading industrial waste water

A technology for the degradation and cultivation of industrial wastewater, applied in the direction of biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of no degradation ability, etc., and achieve the effect of convenient application, wide range and high degradation efficiency

Active Publication Date: 2012-11-07
李钧敏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Wen Hongyu and others described the isolation and physiological characteristics of phenol-degrading bacteria in the Journal of Xuzhou Normal University (Natural Science Edition) in December 2003, Volume 21, No. 4, although it was obtained from industrial wastewater through strain cultivation, domestication and screening. Two strains of bacteria that can degrade phenol were isolated, but the strains only degraded phenol, but did not have the ability to degrade complex pollutants containing both aniline and nitrobenzene compounds in industrial wastewater

Method used

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  • Method for culturing microbial strain for degrading industrial waste water
  • Method for culturing microbial strain for degrading industrial waste water
  • Method for culturing microbial strain for degrading industrial waste water

Examples

Experimental program
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Effect test

Embodiment 1

[0034]Aiming at the aniline, phenol and nitrobenzene compounds in industrial wastewater, the concentration of 0g / L, 0.5g / L, 1.0g / L, 1.5g / L, 2.0g / L, 2.5g / L, 3.0 g / L, 3.5g / L target compound aniline, phenol and nitrobenzene compound liquid medium with different concentration gradients, in which aniline and nitrobenzene compound use it as the only carbon source and nitrogen source, and phenol adds nitrogen in addition Source peptone 1000mg / L, also add other chemical substrate formula potassium nitrate needed for microbial strain growth in the described liquid culture medium: 0.5g / L; Sodium chloride: 1g / L; K 2 HPO 4 ·3H 2 O: 0.8g / L; MgSO 4 ·7H 2 O: 0.2g / L; FeSO 4 ·7H 2 O 0.010g / L, and add part of the liquid medium to add 15g agar (calculated according to 1000ml water) and convert it into a selective solid medium.

[0035] The activated sludge in the industrial wastewater is added to the conventional beef extract peptone liquid medium for enrichment, and then inoculated to the...

Embodiment 2

[0039] Aiming at the aniline, phenol and nitrobenzene compounds in industrial wastewater, the concentration of 0g / L, 0.5g / L, 1.0g / L, 1.5g / L, 2.0g / L, 2.5g / L, 3.0 g / L, 3.5g / L liquid medium of the target compound aniline, phenol and nitrobenzene compounds with different concentration gradients, in which aniline and nitrobenzene compounds use it as the only carbon source and nitrogen source, and phenol is additionally added Nitrogen source ammonium nitrate 1000mg / L, also add other chemical substrate formula potassium nitrate needed for microbial strain growth in the described liquid culture medium: 1.0g / L; Sodium chloride: 0.5g / L; K 2 HPO 4 ·3H 2 O: 0.5g / L; MgSO 4 ·7H 2 O: 0.5g / L; FeSO 4 ·7H 2 O 0.005g / L, and add part of the liquid medium to add 20g agar (calculated by 1000ml water) and convert it into a selective solid medium.

[0040] The activated sludge in the industrial wastewater is added to the conventional beef extract peptone liquid medium for enrichment, and then i...

Embodiment 3

[0044] Aiming at the aniline, phenol and nitrobenzene compounds in industrial wastewater, the concentration of 0g / L, 0.5g / L, 1.0g / L, 1.5g / L, 2.0g / L, 2.5g / L, 3.0 g / L, 3.5g / L liquid medium of the target compound aniline, phenol and nitrobenzene compounds with different concentration gradients, in which aniline and nitrobenzene compounds use it as the only carbon source and nitrogen source, and phenol is additionally added Nitrogen source peptone 1000mg / L, other chemical matrix formulas required for the growth of microbial strains are also added to the liquid medium. Potassium nitrate: 1.5g / L; Sodium chloride: 0.1g / L; K 2 HPO 4 ·3H 2 O: 0.2g / L; MgSO 4 ·7H 2 O: 0.8g / L; FeSO 4 ·7H 2 O 0.015g / L, and add part of the liquid medium to add 20g agar (calculated by 1000ml water) and convert it into a selective solid medium.

[0045] The activated sludge in the industrial wastewater is added to the conventional beef extract peptone liquid medium for enrichment, and then inoculated to...

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Abstract

The invention provides a method for culturing a microbial strain for degrading industrial waste water, which belongs to the field of biotechnology. The method can solve the problems that the existing strain for degrading the industrial waste water has single degradation role and can not degrade compound pollutants. The method for culturing the microbial strain for degrading the industrial waste water comprises the following steps: preparing liquid culture media containing different concentration gradients of target compounds; obtaining a plasmid-containing strain which takes an aniline compound or a nitrobenzene compound as a sole carbon and nitrogen source and takes phenol as a sole carbon source respectively; carrying out acclimatization, culture and identification of plasmid and degradation capability; and purifying for obtaining the plasmid-containing microbial strain for degrading the industrial waste water. The culture method has the advantages of simple process and low cost of the used raw materials, and the cultured strain has the capability of degrading the compound pollutants and has higher efficiency for degrading aniline, phenol and nitrobenzene compounds.

Description

technical field [0001] The invention relates to a method for cultivating microbial strains, in particular to a method for cultivating industrial waste water degrading microbial strains, and belongs to the field of biotechnology. Background technique [0002] Industrial wastewater pollution is a very important and challenging task in the current ecological protection. Organic wastewater from the production processes of printing and dyeing, pharmaceuticals, pesticides, tanning, petrochemical and food industries has complex components and poor biodegradability, which seriously pollutes the water environment and even weakens the long-term reproductive capacity of aquatic organisms. The existing purification treatment technologies for industrial wastewater can be classified into biological methods, chemical methods, physical-chemical coupling methods, and physicochemical-biological coupling methods. Among them, the biological method has the advantages of good economy and no seco...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C02F3/34C02F101/36C02F101/38
Inventor 李钧敏
Owner 李钧敏
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